Angiotensin II induces NF-κB, JNK and p38 MAPK activation in monocytic cells and will increase matrix metalloproteinase-9 expression in a PKC- andRho kinase-dependent method.
- Angiotensin II (ANG II), the primary effector of the renin-angiotensin system, is implicated in endothelial permeability, recruitment and activation of the immune cells, and in addition vascular transforming by way of induction of inflammatory genes. Matrix metalloproteinases (MMPs) are thought of to be necessary inflammatory elements.
HYDROXYSTILBAMIDINE BIS(METHANESULFONATE)
- Elucidation of ANG II signaling pathways and of possible cross-talks between their parts is crucial for the event of environment friendly inhibitory medicines. The present research investigates the inflammatory signaling pathmethods activated by ANG II in cultures of human monocytic U-937 cells, and the results of particular pharmacological inhibitors of signaling intermediates on MMP-9 gene (MMP-9) expression and exercise.
- MMP-9 expression was decided by real-time PCR and supernatants had been analyzed for MMP-9 exercise by ELISA and zymography strategies. A multi-target ELISA package was employed to judge IκB, NF-κB, JNK, p38, and STAT3 activation following remedies.
- Stimulation with ANG II (100 nM) considerably elevated MMP-9 expression and exercise, and in addition activated NF-κB, JNK, and p38 by 3.8-, 2.8- and a pair of.2-fold, respectively (P < 0.01). ANG II-induced MMP-9 expression was considerably diminished by 75 and 67%, respectively, by co-incubation of the cells with a selective inhibitor of protein kinase C (GF109203X, 5 µM) or of rho kinase (Y-27632, 15 µM), however not with inhibitors of phosphoinositide 3-kinase (wortmannin, 200 nM), tyrosine kinases (genistein, 100 µM) or of reactive oxygen species (α-tocopherol, 100 µM).
Angiotensin 1-9 (Ang1-9) Monoclonal Antibody (Human), Biotinylated
- Thus, protein kinase C and Rho kinase are necessary parts of the inflammatory signaling pathways activated by ANG II to extend MMP-9 expression in monocytic cells. Each signaling molecules might represent potential targets for efficient administration of irritation.
Colistin Methanesulfonate (sodium salt) |
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C3046-1000 | ApexBio | 1 g | EUR 208.8 |
Colistin Methanesulfonate (sodium salt) |
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C3046-5000 | ApexBio | 5 g | EUR 734.4 |
Imatinib, Methanesulfonate Salt, >99% |
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BC040-101 | GenDepot | 1g | EUR 278.4 |
Imatinib, Methanesulfonate Salt, >99% |
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BC040-102 | GenDepot | 2g | EUR 360 |
Imatinib, Methanesulfonate Salt, >99% |
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BC040-105 | GenDepot | 5g | EUR 505.2 |
Imatinib, Methanesulfonate Salt, >99% |
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BC040-500 | GenDepot | 500mg | Ask for price |
Obatoclax |
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27044 | BPS Bioscience | 5 mg | EUR 120 |
Obatoclax (Mesylate) |
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HY-10969 | MedChemExpress | 100mg | EUR 655.2 |
Obatoclax (GX15-070) |
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E1KS1057 | EnoGene | 5 mg | EUR 1333.2 |
Pridinol Methanesulfonate |
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A8507-1000 | ApexBio | 1 g | EUR 517.2 |
Pridinol Methanesulfonate |
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A8507-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 129.6 |
Pridinol Methanesulfonate |
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A8507-50 | ApexBio | 50 mg | EUR 153.6 |
Pridinol Methanesulfonate |
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A8507-5000 | ApexBio | 5 g | EUR 1198.8 |
Ethyl methanesulfonate |
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20-abx186442 | Abbexa |
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Abemaciclib (methanesulfonate) |
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HY-16297 | MedChemExpress | 500mg | EUR 542.4 |
Ethyl methanesulfonate |
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GX0446-100G | Glentham Life Sciences | 100 g | EUR 313.2 |
Ethyl methanesulfonate |
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GX0446-10G | Glentham Life Sciences | 10 g | EUR 88.8 |
Ethyl methanesulfonate |
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GX0446-25G | Glentham Life Sciences | 25 g | EUR 141.6 |
Ethyl methanesulfonate |
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GX0446-5G | Glentham Life Sciences | 5 g | EUR 64.8 |
Silver methanesulfonate |
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GX5020-25G | Glentham Life Sciences | 25 g | EUR 208.8 |
Silver methanesulfonate |
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GX5020-5G | Glentham Life Sciences | 5 g | EUR 85.2 |
Tricaine methanesulfonate |
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GE5936-10G | Glentham Life Sciences | 10 g | EUR 68.4 |
Tricaine methanesulfonate |
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GE5936-25G | Glentham Life Sciences | 25 g | EUR 98.4 |
Tricaine methanesulfonate |
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GE5936-50G | Glentham Life Sciences | 50 g | EUR 141.6 |
Tricaine methanesulfonate |
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GE5936-5G | Glentham Life Sciences | 5 g | EUR 56.4 |
Ethyl methanesulfonate |
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S-1930 | Scientific Laboratory Supplies | 1ML | EUR 75.24 |
Obatoclax mesylate (GX15-070) |
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A4194-10 | ApexBio | 10 mg | EUR 331.2 |
Obatoclax mesylate (GX15-070) |
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A4194-100 | ApexBio | 100 mg | EUR 1534.8 |
Obatoclax mesylate (GX15-070) |
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A4194-25 | ApexBio | 25 mg | EUR 582 |
Obatoclax mesylate (GX15-070) |
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A4194-5 | ApexBio | 5 mg | EUR 212.4 |
Obatoclax mesylate (GX15-070) |
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A4194-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 243.6 |
Obatoclax mesylate (GX15-070) |
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A4194-50 | ApexBio | 50 mg | EUR 796.8 |
Obatoclax mesylate (GX15-070) |
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A4194-S | ApexBio | Evaluation Sample | EUR 97.2 |
Colistin Sodium Methanesulfonate |
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C073-10G | TOKU-E | 10 g | EUR 984 |
Colistin Sodium Methanesulfonate |
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C073-1G | TOKU-E | 1 g | EUR 162 |
Colistin Sodium Methanesulfonate |
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C073-5G | TOKU-E | 5 g | EUR 528 |
Tariquidar methanesulfonate, hydrate |
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A3859-10 | ApexBio | 10 mg | EUR 226.8 |
Tariquidar methanesulfonate, hydrate |
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A3859-50 | ApexBio | 50 mg | EUR 532.8 |
PF-04217903 methanesulfonate |
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A3712-100 | ApexBio | 100 mg | EUR 714 |
PF-04217903 methanesulfonate |
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A3712-25 | ApexBio | 25 mg | EUR 351.6 |
PF-04217903 methanesulfonate |
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A3712-5 | ApexBio | 5 mg | EUR 157.2 |
2-Chloroethyl methanesulfonate |
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20-abx183170 | Abbexa |
|
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Colistin sodium methanesulfonate |
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GA2961-1G | Glentham Life Sciences | 1 g | EUR 151.2 |
Colistin sodium methanesulfonate |
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GA2961-5G | Glentham Life Sciences | 5 g | EUR 351.6 |
Tariquidar (methanesulfonate, hydrate) |
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HY-10550A | MedChemExpress | 100mg | EUR 734.4 |
Hydroxystilbamidine bis(methanesulfonate) |
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HY-108166A | MedChemExpress | 5mg | EUR 639.6 |
PF-06424439 (methanesulfonate) |
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HY-108341A | MedChemExpress | 10mM/1mL | EUR 175.2 |
PF-04217903 (methanesulfonate) |
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HY-12017A | MedChemExpress | 10mM/1mL | EUR 187.2 |
BCX 1470 (methanesulfonate) |
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HY-50875 | MedChemExpress | 10mM/1mL | EUR 696 |
Ethyl 3-Aminobenzoate (methanesulfonate) |
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C3619-100000 | ApexBio | 100 g | EUR 381.6 |
Ethyl 3-Aminobenzoate (methanesulfonate) |
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C3619-25000 | ApexBio | 25 g | EUR 153.6 |
Ethyl 3-Aminobenzoate (methanesulfonate) |
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C3619-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 135.6 |
Ethyl 3-Aminobenzoate (methanesulfonate) |
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C3619-50000 | ApexBio | 50 g | EUR 234 |
Ro 31-8220 methanesulfonate |
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1946-1 | Biovision | each | EUR 164.4 |
Ro 31-8220 methanesulfonate |
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1946-5 | Biovision | each | EUR 457.2 |
Pesticide Std Ethyl methanesulfonate Neat |
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REPET352N | Scientific Laboratory Supplies | 10MG | EUR 72.96 |
Pesticide Std Methyl Methanesulfonate Neat |
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REPET374N | Scientific Laboratory Supplies | 10MG | EUR 72.96 |
Pesticide Std Ethyl methanesulfonate 1000ug/mL in MeOH |
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REPET352 | Scientific Laboratory Supplies | 1ML | EUR 88.92 |
Pesticide Std Ethyl methanesulfonate 2000ug/mL in MeOH |
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REPET353 | Scientific Laboratory Supplies | 1ML | EUR 99.18 |
Pesticide Std Methyl Methanesulfonate 1000ug/mL in MeOH |
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REPET374 | Scientific Laboratory Supplies | 1ML | EUR 98.04 |
Pesticide Std Methyl Methanesulfonate 2000ug/mL in MeOH |
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REPET375 | Scientific Laboratory Supplies | 1ML | EUR 94.62 |
Bile salt |
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BB0225 | Bio Basic | 25g | EUR 95.5 |
Mannitol Salt |
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254027 | Scientific Laboratory Supplies | PK20 | EUR 13.68 |
CAPS, sodium salt |
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CB0097 | Bio Basic | 50g | EUR 86.1 |
CAPSO, sodium salt |
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CD0041 | Bio Basic | 50g | EUR 91.32 |
Hepes, Sodium Salt |
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CH039 | ABM | 25 g | EUR 141.6 |
Hepes, Sodium Salt |
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CH040 | ABM | 100 g | EUR 202.8 |
Hepes, Sodium Salt |
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CH041 | ABM | 500 g | EUR 448.8 |
Cefamandole sodium salt |
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C079-100MG | TOKU-E | 100 mg | EUR 177.6 |
Cefamandole sodium salt |
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C079-500MG | TOKU-E | 500 mg | EUR 568.8 |
Chlorhexidine diacetate salt |
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C200-25G | TOKU-E | 25 g | EUR 216 |
TAK-700 salt |
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A3852-10 | ApexBio | 10 mg | EUR 514.8 |
Angiotensin II induces NF-κB, JNK and p38 MAPK activation in monocytic cells and will increase matrix metalloproteinase-9 expression in a PKC- andRho kinase-dependent method.
- Angiotensin II (ANG II), the primary effector of the renin-angiotensin system, is implicated in endothelial permeability, recruitment and activation of the immune cells, and in addition vascular transforming by way of induction of inflammatory genes. Matrix metalloproteinases (MMPs) are thought of to be necessary inflammatory elements.
Mouse pre-microRNA Expression Assemble mir-9-1
- Elucidation of ANG II signaling pathways and of potential cross-talks between their components is crucial for the event of environment friendly inhibitory medicines.
- The present research investigates the inflammatory signaling pathways activated by ANG II in cultures of human monocytic U-937 cells, and the results of particular pharmacological inhibitors of signaling intermediates on MMP-9 gene (MMP-9) expression and exercise. MMP-9 expression was decided by real-time PCR and supernatants had been analyzed for MMP-9 exercise by ELISA and zymography strategies.
- A multi-target ELISA package was employed to judge IκB, NF-κB, JNK, p38, and STAT3 activation following remedies. Stimulation with ANG II (100 nM) considerably elevated MMP-9 expression and exercise, and in addition activated NF-κB, JNK, and p38 by 3.8-, 2.8- and a pair of.2-fold, respectively (P < 0.01).
Human Angiotensin 1-9 (Ang1-9) ELISA Kit |
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DLR-Ang1-9-Hu-48T | DL Develop | 48T | EUR 601.2 |
Human Angiotensin 1-9 (Ang1-9) ELISA Kit |
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DLR-Ang1-9-Hu-96T | DL Develop | 96T | EUR 781.2 |
Human Angiotensin 1-9 (Ang1-9) ELISA Kit |
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RDR-Ang1-9-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 630 |
Human Angiotensin 1-9 (Ang1-9) ELISA Kit |
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RDR-Ang1-9-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 874.8 |
Human Angiotensin 1-9 (Ang1-9) ELISA Kit |
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RD-Ang1-9-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 603.6 |
Human Angiotensin 1-9 (Ang1-9) ELISA Kit |
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RD-Ang1-9-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 836.4 |
Angiotensin 1-9 (Ang1-9) Monoclonal Antibody (Human) |
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4-MAT844Hu21 | Cloud-Clone |
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Angiotensin 1-9 (Ang1-9) Antibody |
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20-abx132109 | Abbexa |
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Rat Angiotensin 1-9(Ang1-9) |
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QY-E11784 | Qayee Biotechnology | 96T | EUR 448.8 |
Angiotensin 1-9 (Ang1-9) Monoclonal Antibody (Human), APC |
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4-MAT844Hu21-APC | Cloud-Clone |
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Angiotensin 1-9 (Ang1-9) Monoclonal Antibody (Human), Biotinylated |
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4-MAT844Hu21-Biotin | Cloud-Clone |
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Angiotensin 1-9 (Ang1-9) Monoclonal Antibody (Human), Cy3 |
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4-MAT844Hu21-Cy3 | Cloud-Clone |
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Angiotensin 1-9 (Ang1-9) Monoclonal Antibody (Human), FITC |
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4-MAT844Hu21-FITC | Cloud-Clone |
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Angiotensin 1-9 (Ang1-9) Monoclonal Antibody (Human), HRP |
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4-MAT844Hu21-HRP | Cloud-Clone |
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Angiotensin 1-9 (Ang1-9) Monoclonal Antibody (Human), PE |
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4-MAT844Hu21-PE | Cloud-Clone |
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Mouse Angiotensin 1-9 (Ang1-9) ELISA Kit |
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abx353312-96tests | Abbexa | 96 tests | EUR 943.2 |
Angiotensin 1-9 (Ang1-9) Monoclonal Antibody (Human), APC-Cy7 |
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4-MAT844Hu21-APC-Cy7 | Cloud-Clone |
|
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Angiotensin 1-9 (Ang1-9) CLIA Kit |
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20-abx490554 | Abbexa |
|
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Angiotensin 1-9 (Ang1-9) ELISA Kit |
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20-abx258341 | Abbexa |
|
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Human Angiotensin 1-9 (Ang1-9) ELISA Kit |
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CET844Hu-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 5991.96 |
Human Angiotensin 1-9 (Ang1-9) ELISA Kit |
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CET844Hu-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 598.63 |
Human Angiotensin 1-9 (Ang1-9) ELISA Kit |
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CET844Hu-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 803.76 |
Human Angiotensin 1-9 (Ang1-9) ELISA Kit |
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CET844Hu-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 3256.92 |
Human Angiotensin 1-9 (Ang1-9) ELISA Kit |
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4-CET844Hu | Cloud-Clone |
|
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Rat Ang1-9(Angiotensin 1-9) ELISA Kit |
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ER1617 | FN Test | 96T | EUR 681.12 |
Human Angiotensin 1-9 (Ang1-9)ELISA Kit |
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201-12-2829 | SunredBio | 96 tests | EUR 528 |
Human Angiotensin 1-9 (Ang1-9) ELISA Kit |
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20-abx150652 | Abbexa |
|
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Human Angiotensin 1-9 (Ang1-9) ELISA Kit |
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abx575059-96tests | Abbexa | 96 tests | EUR 801.6 |
Human Angiotensin 1-9 (Ang1-9) Peptide (OVA) |
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20-abx655870 | Abbexa |
|
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Human Angiotensin 1-9 (Ang1-9) CLIA Kit |
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20-abx490553 | Abbexa |
|
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Rat Angiotensin 1-9 (Ang1-9) ELISA Kit |
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abx257809-96tests | Abbexa | 96 tests | EUR 895.2 |
Human Angiotensin 1-9(Ang1-9)ELISA Kit |
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QY-E00730 | Qayee Biotechnology | 96T | EUR 433.2 |
Human Angiotensin 1-9 (Ang1-9)ELISA Kit |
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YLA0066HU-48T | Shanghai YL Biotech | 48T | EUR 435 |
Human Angiotensin 1-9 (Ang1-9)ELISA Kit |
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YLA0066HU-96T | Shanghai YL Biotech | 96T | EUR 562.5 |
ELISA kit for Mouse Ang1-9 (Angiotensin 1-9) Kit |
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E-EL-M2682 | Elabscience Biotech | 1 plate of 96 wells | EUR 640.8 |
Multi-species Angiotensin 1-9 (Ang1-9) ELISA Kit |
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CET844Mi-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 6282.56 |
Multi-species Angiotensin 1-9 (Ang1-9) ELISA Kit |
|||
CET844Mi-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 622.56 |
Multi-species Angiotensin 1-9 (Ang1-9) ELISA Kit |
|||
CET844Mi-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 837.95 |
Multi-species Angiotensin 1-9 (Ang1-9) ELISA Kit |
|||
CET844Mi-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 3410.77 |
Multi-species Angiotensin 1-9 (Ang1-9) ELISA Kit |
|||
4-CET844Mi | Cloud-Clone |
|
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ELISA kit for Human Ang1-9 (Angiotensin 1-9) |
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ELK7609 | ELK Biotech | 1 plate of 96 wells | EUR 446.4 |
ELISA kit for Human Ang1-9 (Angiotensin 1-9) |
|||
ELK7679 | ELK Biotech | 1 plate of 96 wells | EUR 518.4 |
Pesticide Mix 9 Containing 16 Compounds in ACN:Acetone (9:1) |
|||
SPXPR-9 | Scientific Laboratory Supplies | 1ML | EUR 250.8 |
Ang1-9 ELISA Kit| Rat Angiotensin 1-9 ELISA Kit |
|||
EF018213 | Lifescience Market | 96 Tests | EUR 826.8 |
7095B 9 PASTEUR PIPET |
|||
7095B-9 | CORNING | 200/pk | EUR 84 |
Individual Reaction Mix 9 |
|||
G065-9 | ABM | 200 reactions | EUR 200.4 |
Mouse pre-microRNA Expression Construct mir-9-1 |
|||
MMIR-9-1-PA-1 | SBI | Bacterial Streak | EUR 820.8 |
7095D 9 PASTEUR PIPET BOROSILICATE |
|||
7095D-9 | CORNING | 200/pk | EUR 93.6 |
Angiotensin (1-9) |
|||
5-00677 | CHI Scientific | 4 x 5mg | Ask for price |
Mouse Brain Tissue Preparation Buffer 9: Normal Nerve Microglia |
|||
9-80132 | CHI Scientific | 1 x 100 ml | Ask for price |
Mouse Angiotensin 1-7 (Ang1-7) ELISA Kit |
|||
DLR-Ang1-7-Mu-48T | DL Develop | 48T | EUR 614.4 |
Mouse Angiotensin 1-7 (Ang1-7) ELISA Kit |
|||
DLR-Ang1-7-Mu-96T | DL Develop | 96T | EUR 800.4 |
Mouse Angiotensin 1-7 (Ang1-7) ELISA Kit |
|||
RDR-Ang1-7-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 646.8 |
Mouse Angiotensin 1-7 (Ang1-7) ELISA Kit |
|||
RDR-Ang1-7-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 898.8 |
Mouse Angiotensin 1-7 (Ang1-7) ELISA Kit |
|||
RD-Ang1-7-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 619.2 |
Mouse Angiotensin 1-7 (Ang1-7) ELISA Kit |
|||
RD-Ang1-7-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 859.2 |
Mouse Angiotensin (1- 9), ANG (1- 9) ELISA Kit |
|||
CELI-66004m | Lifescience Market | 96 Tests | EUR 1038 |
Angiotensin (1-9) Peptide |
|||
20-abx266137 | Abbexa |
|
|
Angiotensin I (1-9) |
|||
H-5038.0025 | Bachem | 25.0mg | EUR 327.6 |
Angiotensin I (1-9) |
|||
H-5038.0100 | Bachem | 100.0mg | EUR 907.2 |
Human Brain Tissue Preparation Buffer 9: Normal Nerve Microglia |
|||
9-80021 | CHI Scientific | 1 x 100 ml | Ask for price |
Human Cancer Tissue Preparation Buffer 9: Kidney Tumor Cells |
|||
9-80032 | CHI Scientific | 1 x 100 ml | Ask for price |
Rat Brain Tissue Preparation Buffer 9: Normal Neuron Cells |
|||
9-80227 | CHI Scientific | 1 x 100 ml | Ask for price |
Anti-pro Caspase 9 Rabbit Monoclonal Antibody |
|||
M00080-1 | BosterBio | 100ug/vial | EUR 476.4 |
Mouse pre-microRNA Expression Construct mir-9-2 |
|||
MMIR-9-2-PA-1 | SBI | Bacterial Streak | EUR 820.8 |
Mouse pre-microRNA Expression Construct mir-9-3 |
|||
MMIR-9-3-PA-1 | SBI | Bacterial Streak | EUR 820.8 |
Angiotensin 1/2 (1-9) |
|||
A1007-10 | ApexBio | 10 mg | EUR 199.2 |
Angiotensin 1/2 (1-9) |
|||
A1007-25 | ApexBio | 25 mg | EUR 254.4 |
Angiotensin 1/2 (1-9) |
|||
A1007-5 | ApexBio | 5 mg | EUR 142.8 |
Anti-ZnT-9 Antibody |
|||
A10142-1 | BosterBio | 100ul | EUR 476.4 |
Anti-BMP-9 Antibody |
|||
A03443-1 | BosterBio | 100ul | EUR 476.4 |
Anti-NHE-9 Antibody |
|||
A05959-1 | BosterBio | 100ul | EUR 476.4 |
Canine Angiotensin 1-7 (Ang1-7) ELISA Kit |
|||
DLR-Ang1-7-c-48T | DL Develop | 48T | EUR 672 |
- ANG II-induced MMP-9 expression was considerably diminished by 75 and 67%, respectively, by co-incubation of the cells with a selective inhibitor of protein kinase C (GF109203X, 5 µM) or of rho kinase (Y-27632, 15 µM), however not with inhibitors of phosphoinositide 3-kinase (wortmannin, 200 nM), tyrosine kinases (genistein, 100 µM) or of reactive oxygen species (α-tocopherol, 100 µM).
TARIQUIDAR METHANESULFONATE, HYDRATE
- Thus, protein kinase C and Rho kinase are necessary parts of the inflammatory signaling pathways activated by ANG II to extend MMP-9 expression in monocytic cells. Each signaling molecules might represent potential targets for efficient management of irritation.