Validation and comparability of luminex multiplex cytokine evaluation kits with ELISA: determinations of a panel of 9 cytokines in scientific pattern tradition supernatants.
OBJECTIVE
Analyses of the expression sample of a number of cytokines are incessantly required for characterization of the standing of the immune system because it pertains to Th kind bias and intrinsic ranges of irritation. Classically, evaluation of cytokine expression patterns has been carried out by enzyme-linked immunosorbent assays (ELISA) for every separate analyte.
A brand new expertise, Luminex MAP, facilitates the simultaneous analysis of a number of immune mediators with benefits of upper throughput, smaller pattern quantity, and decrease price. Validation of this expertise has been restricted to small pattern units, restricted use of scientific research specimens, and use of non-commercial reagents.
COLISTIN METHANESULFONATE (SODIUM SALT)
METHODS
Ninety-six specimens from ladies over the course of their respective pregnancies had been evaluated for cytokine concentrations utilizing commercially obtainable ELISA kits and commercially available Luminex MAP kits according to the producers’ instructions. Correlations between information units had been evaluated utilizing Pearson’s correlation coefficient (r).
RESULTS
Wonderful correlations were demonstrated for IL-1 beta, IL-4, IL-5, IL-6, IL-10, IFN gamma, and TNF alpha, in distinction to IL-12 p 70 and IL-13.
COLISTIN SODIUM METHANESULFONATE
CONCLUSIONS
Luminex multiplex expertise has distinct benefits and is a sound various technique to ELISA for the analysis of the vast majority of cytokines examined and for the characterization of immune system standing.
Dabrafenib, Methanesulfonate Salt [CAS# 1195768-06-9] |
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| D-5699 | LC LABORATORIES | 10 mg | EUR 44 |
Metaphit Methanesulfonate Salt |
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| M258810 | Toronto Research Chemicals | 250mg | EUR 213 |
Phenamil Methanesulfonate Salt |
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| P294780 | Toronto Research Chemicals | 10mg | EUR 58 |
Pridinol Methanesulfonate Salt |
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| P715000 | Toronto Research Chemicals | 100mg | EUR 679 |
Phentolamine Methanesulfonate Salt |
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| P302560 | Toronto Research Chemicals | 100mg | EUR 86 |
BAY 57-1293 Methanesulfonate Salt |
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| B118805 | Toronto Research Chemicals | 5mg | EUR 82 |
Imatinib, Methanesulfonate Salt, >99% |
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| BC040-101 | GenDepot | 1g | EUR 278.4 |
Imatinib, Methanesulfonate Salt, >99% |
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| BC040-102 | GenDepot | 2g | EUR 360 |
Imatinib, Methanesulfonate Salt, >99% |
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| BC040-105 | GenDepot | 5g | EUR 505.2 |
Imatinib, Methanesulfonate Salt, >99% |
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| BC040-500 | GenDepot | 500mg | Ask for price |
Metaphit Methanesulfonate Salt (1-(1-[3-Isothiocyanato]phenyl)cyclohexylpiperidine, Methanesulfonate Salt) |
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| MBS6089256-25mg | MyBiosource | 2.5(mg | EUR 575 |
Metaphit Methanesulfonate Salt (1-(1-[3-Isothiocyanato]phenyl)cyclohexylpiperidine, Methanesulfonate Salt) |
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| MBS6089256-5x25mg | MyBiosource | 5x2.5mg | EUR 2440 |
BAY 57-1293 Methanesulfonate Salt-d4 |
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| B540591 | Toronto Research Chemicals | 50mg | EUR 9200 |
Pridinol Methanesulfonate Salt (a,a-Diphenyl-1-piperidinepropanol Methanesulfonate) |
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| MBS6048097-100mg | MyBiosource | 100(mg | EUR 505 |
Pridinol Methanesulfonate Salt (a,a-Diphenyl-1-piperidinepropanol Methanesulfonate) |
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| MBS6048097-5x100mg | MyBiosource | 5x100mg | EUR 2125 |
Colistin Methanesulfonate (sodium salt) |
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| C3046-1000 | ApexBio | 1 g | EUR 122 |
Colistin Methanesulfonate (sodium salt) |
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| C3046-5000 | ApexBio | 5 g | EUR 547 |
Colistin methanesulfonate sodium salt |
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| T21518-10mg | TargetMol Chemicals | 10mg | Ask for price |
Colistin methanesulfonate sodium salt |
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| T21518-1g | TargetMol Chemicals | 1g | Ask for price |
Colistin methanesulfonate sodium salt |
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| T21518-1mg | TargetMol Chemicals | 1mg | Ask for price |
Colistin methanesulfonate sodium salt |
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| T21518-50mg | TargetMol Chemicals | 50mg | Ask for price |
Colistin methanesulfonate sodium salt |
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| T21518-5mg | TargetMol Chemicals | 5mg | Ask for price |
Colistin Methanesulfonate (sodium salt) |
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| MBS5756763-100mg | MyBiosource | 100mg | EUR 160 |
Colistin Methanesulfonate (sodium salt) |
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| MBS5756763-200mg | MyBiosource | 200mg | EUR 190 |
Colistin Methanesulfonate (sodium salt) |
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| MBS5756763-500mg | MyBiosource | 500mg | EUR 270 |
Colistin Methanesulfonate (sodium salt) |
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| MBS5756763-50mg | MyBiosource | 50mg | EUR 145 |
Colistin Methanesulfonate (sodium salt) |
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| MBS5756763-5mg | MyBiosource | 5mg | EUR 125 |
Colistin methanesulfonate (sodium salt) |
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| HY-A0214 | MedChemExpress | 100 mg | EUR 54.11 |
Imatinib, Methanesulfonate Salt [CAS# 220127-57-1] |
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| I-5508 | LC LABORATORIES | 1 g | EUR 48 |
Nelfinavir, Methanesulfonate Salt [CAS# 159989-65-8] |
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| N-3883 | LC LABORATORIES | 10 mg | EUR 45 |
Phentolamine, Methanesulfonate Salt (2-[N-(m-Hydroxyphenyl)-p-toluidinomethyl]imidazoline, Methanesulfonate) |
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| MBS6048058-250mg | MyBiosource | 250(mg | EUR 500 |
Phentolamine, Methanesulfonate Salt (2-[N-(m-Hydroxyphenyl)-p-toluidinomethyl]imidazoline, Methanesulfonate) |
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| MBS6048058-5x250mg | MyBiosource | 5x250mg | EUR 2090 |
3,4-Dihydro-2H,10H-azepino[3,4-b]indole-1,5-dione Methanesulfonate Salt |
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| MBS6046336-5mg | MyBiosource | 5(mg | EUR 525 |
3,4-Dihydro-2H,10H-azepino[3,4-b]indole-1,5-dione Methanesulfonate Salt |
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| MBS6046336-5x5mg | MyBiosource | 5x5(mg | EUR 2210 |
Ethyl Methanesulfonate |
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| 15519-54 | NACALAI TESQUE | 5G | EUR 42.7 |
Ethyl methanesulfonate |
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| 20-abx186442 | Abbexa |
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Ethyl Methanesulfonate |
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| E917995 | Toronto Research Chemicals | 100g | EUR 126 |
Butyl Methanesulfonate |
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| B694095 | Toronto Research Chemicals | 1g | EUR 230 |
Ethyl methanesulfonate |
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| GX0446 | Glentham Life Sciences | 5g | EUR 209.98 |
Hexyl Methanesulfonate |
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| H297155 | Toronto Research Chemicals | 1g | EUR 187 |
Ethyl methanesulfonate |
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| GX0446-10 | Glentham Life Sciences | 10 | EUR 43.5 |
Ethyl methanesulfonate |
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| GX0446-100 | Glentham Life Sciences | 100 | EUR 229.3 |
Ethyl methanesulfonate |
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| GX0446-100G | Glentham Life Sciences | 100 g | EUR 313.2 |
Ethyl methanesulfonate |
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| GX0446-10G | Glentham Life Sciences | 10 g | EUR 88.8 |
Ethyl methanesulfonate |
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| GX0446-25 | Glentham Life Sciences | 25 | EUR 87.1 |
Ethyl methanesulfonate |
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| GX0446-25G | Glentham Life Sciences | 25 g | EUR 141.6 |
Ethyl methanesulfonate |
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| GX0446-5 | Glentham Life Sciences | 5 | EUR 23.8 |
Ethyl methanesulfonate |
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| GX0446-5G | Glentham Life Sciences | 5 g | EUR 64.8 |
CYH33 methanesulfonate |
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| T38979-10mg | TargetMol Chemicals | 10mg | Ask for price |
CYH33 methanesulfonate |
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| T38979-1g | TargetMol Chemicals | 1g | Ask for price |
CYH33 methanesulfonate |
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| T38979-1mg | TargetMol Chemicals | 1mg | Ask for price |
CYH33 methanesulfonate |
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| T38979-50mg | TargetMol Chemicals | 50mg | Ask for price |
CYH33 methanesulfonate |
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| T38979-5mg | TargetMol Chemicals | 5mg | Ask for price |
CYH33 methanesulfonate |
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| MBS5799537-10mg | MyBiosource | 10mg | EUR 1395 |
CYH33 methanesulfonate |
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| MBS5799537-5mg | MyBiosource | 5(mg | EUR 925 |
CYH33 methanesulfonate |
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| MBS5799537-5x10mg | MyBiosource | 5x10(mg | EUR 6120 |
CYH33 (methanesulfonate) |
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| HY-123938A | MedChemExpress | 10 mg | EUR 5140.77 |
Methyl Methanesulfonate |
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| 22801-14 | NACALAI TESQUE | 10G | EUR 44.8 |
Methyl methanesulfonate |
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| T9985-10mg | TargetMol Chemicals | 10mg | Ask for price |
Methyl methanesulfonate |
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| T9985-1g | TargetMol Chemicals | 1g | Ask for price |
Methyl methanesulfonate |
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| T9985-1mg | TargetMol Chemicals | 1mg | Ask for price |
Methyl methanesulfonate |
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| T9985-50mg | TargetMol Chemicals | 50mg | Ask for price |
Methyl methanesulfonate |
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| T9985-5mg | TargetMol Chemicals | 5mg | Ask for price |
Methyl Methanesulfonate |
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| M320170 | Toronto Research Chemicals | 10g | EUR 86 |
Methyl methanesulfonate |
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| MBS5786682-10mg | MyBiosource | 10mg | EUR 160 |
Methyl methanesulfonate |
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| MBS5786682-25mg | MyBiosource | 25mg | EUR 200 |
Methyl methanesulfonate |
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| MBS5786682-50mg | MyBiosource | 50(mg | EUR 250 |
Methyl methanesulfonate |
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| MBS5786682-5mg | MyBiosource | 5(mg | EUR 145 |
Propyl Methanesulfonate |
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| B523550 | Toronto Research Chemicals | 100mg | EUR 63 |
Acetyl Methanesulfonate |
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| A173895 | Toronto Research Chemicals | 10g | EUR 489 |
Silver methanesulfonate |
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| GX5020 | Glentham Life Sciences | 5g | EUR 130.36 |
Silver methanesulfonate |
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| GX5020-25 | Glentham Life Sciences | 25 | EUR 142.4 |
Silver methanesulfonate |
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| GX5020-25G | Glentham Life Sciences | 25 g | EUR 208.8 |
Silver methanesulfonate |
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| GX5020-5 | Glentham Life Sciences | 5 | EUR 40.3 |
Silver methanesulfonate |
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| GX5020-5G | Glentham Life Sciences | 5 g | EUR 85.2 |
BCX 1470 (methanesulfonate) |
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| HY-50875 | MedChemExpress | 10mM/1mL | EUR 696 |
BCX 1470 methanesulfonate |
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| T13567-10mg | TargetMol Chemicals | 10mg | Ask for price |
BCX 1470 methanesulfonate |
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| T13567-1g | TargetMol Chemicals | 1g | Ask for price |
BCX 1470 methanesulfonate |
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| T13567-1mg | TargetMol Chemicals | 1mg | Ask for price |
BCX 1470 methanesulfonate |
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| T13567-50mg | TargetMol Chemicals | 50mg | Ask for price |
BCX 1470 methanesulfonate |
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| T13567-5mg | TargetMol Chemicals | 5mg | Ask for price |
BCX 1470 methanesulfonate |
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| MBS386704-10mg | MyBiosource | 10mg | EUR 485 |
BCX 1470 methanesulfonate |
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| MBS386704-25mg | MyBiosource | 25mg | EUR 870 |
BCX 1470 methanesulfonate |
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| MBS386704-2mg | MyBiosource | 2mg | EUR 270 |
BCX 1470 methanesulfonate |
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| MBS386704-5mg | MyBiosource | 5mg | EUR 345 |
BCX 1470 methanesulfonate |
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| MBS386704-5x25mg | MyBiosource | 5x25mg | EUR 3895 |
BCX 1470 methanesulfonate |
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| MBS5754249-25mg | MyBiosource | 25mg | EUR 1165 |
Purposeful evaluation of the classical, various, and MBL pathways of the complement system: standardization and validation of a easy ELISA.
- Major defence towards invading microorganisms depends upon a practical innate immune system and the complement system performs a serious position in such immunity.
- Deficiencies in one of many parts of the complement system may cause extreme and recurrent infections, systemic ailments, akin to systemic lupus erythematosus (SLE) and renal illness.
Rapamycin |
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| 20-abx076788 | Abbexa |
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Rapamycin |
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| 1568-5 | Biovision | each | EUR 339.6 |
Rapamycin |
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| 1568-50 | Biovision | each | EUR 1396.8 |
Rapamycin |
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| 30037-94 | NACALAI TESQUE | 100UG | EUR 138.6 |
Rapamycin |
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| 27062 | BPS Bioscience | 50 mg | EUR 135 |
Rapamycin |
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| 41810000-1 | Glycomatrix | 10 mg | EUR 52.16 |
Rapamycin |
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| 41810000-2 | Glycomatrix | 100 mg | EUR 161.05 |
Rapamycin |
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| 41810000-3 | Glycomatrix | 50 mg | EUR 89.68 |
Rapamycin |
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| 41810000-4 | Glycomatrix | 500 mg | EUR 364.2 |
Rapamycin |
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| 20-abx185560 | Abbexa |
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Rapamycin |
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| E1B1842 | EnoGene | 1mg | EUR 60 |
Rapamycin |
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| tlrl-rap-5 | InvivoGen FR | 5 x 5 mg | EUR 248.85 |
Rapamycin |
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| GA7417 | Glentham Life Sciences | 5mg | EUR 181.03 |
Rapamycin |
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| G613 | ABM | 100 mg | EUR 800 |
Rapamycin |
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| MBS132175-100mg | MyBiosource | 100mg | EUR 1065 |
Rapamycin |
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| MBS132175-500mg | MyBiosource | 500mg | EUR 2775 |
Rapamycin |
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| HY-10219 | MedChemExpress | 200mg | EUR 241.2 |
Rapamycin |
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| GA7417-100 | Glentham Life Sciences | 100 | EUR 197.7 |
Rapamycin |
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| GA7417-100MG | Glentham Life Sciences | 100 mg | EUR 274.8 |
Rapamycin |
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| GA7417-25 | Glentham Life Sciences | 25 | EUR 63.3 |
Rapamycin |
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| GA7417-25MG | Glentham Life Sciences | 25 mg | EUR 112.8 |
Rapamycin |
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| GA7417-5 | Glentham Life Sciences | 5 | EUR 23.8 |
Rapamycin |
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| GA7417-50 | Glentham Life Sciences | 50 | EUR 118.7 |
Rapamycin |
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| GA7417-50MG | Glentham Life Sciences | 50 mg | EUR 180 |
Rapamycin |
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| GA7417-5MG | Glentham Life Sciences | 5 mg | EUR 64.8 |
Rapamycin |
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| R001-10MG | TOKU-E | 10 mg | EUR 149.37 |
Rapamycin |
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| R001-25MG | TOKU-E | 25 mg | EUR 187.13 |
Rapamycin |
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| SIH-212-25MG | Stressmarq | 25 mg | EUR 345 |
Rapamycin |
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| SIH-212-5MG | Stressmarq | 5 mg | EUR 138 |
Rapamycin |
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| T1537-10mg | TargetMol Chemicals | 10mg | Ask for price |
Rapamycin |
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| T1537-1g | TargetMol Chemicals | 1g | Ask for price |
Rapamycin |
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| T1537-1mg | TargetMol Chemicals | 1mg | Ask for price |
Rapamycin |
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| T1537-50mg | TargetMol Chemicals | 50mg | Ask for price |
Rapamycin |
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| T1537-5mg | TargetMol Chemicals | 5mg | Ask for price |
Rapamycin |
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| abx076788-100l | Abbexa | 100 µl | EUR 118.75 |
Rapamycin |
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| abx076788-1ml | Abbexa | 1 ml | Ask for price |
Rapamycin |
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| abx076788-200l | Abbexa | 200 µl | EUR 300 |
Rapamycin |
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| MBS696245-10mg | MyBiosource | 10mg | EUR 235 |
Rapamycin |
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| MBS696245-1mg | MyBiosource | 1mg | EUR 135 |
Rapamycin |
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| MBS696245-5x10mg | MyBiosource | 5x10mg | EUR 1005 |
Rapamycin |
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| MBS807957-25mg | MyBiosource | 25mg | EUR 450 |
Rapamycin |
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| MBS807957-5mg | MyBiosource | 5mg | EUR 260 |
Rapamycin |
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| MBS807957-5x25mg | MyBiosource | 5x25mg | EUR 1770 |
Rapamycin |
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| MBS807957-5x5mg | MyBiosource | 5x5mg | EUR 905 |
Rapamycin |
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| MBS8579821-1mg | MyBiosource | 1mg | EUR 155 |
Rapamycin |
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| MBS8579821-5x1mg | MyBiosource | 5x1mg | EUR 555 |
Rapamycin |
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| MBS407517-100mg | MyBiosource | 100mg | EUR 175 |
Rapamycin |
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| MBS407517-1g | MyBiosource | 1g | EUR 450 |
Rapamycin |
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| MBS407517-5x100mg | MyBiosource | 5x100mg | EUR 730 |
Rapamycin |
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| MBS407517-5x1g | MyBiosource | 5x1g | EUR 1970 |
Rapamycin |
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| MBS3604884-100mg | MyBiosource | 100mg | EUR 210 |
Rapamycin |
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| MBS3604884-200mg | MyBiosource | 200mg | EUR 240 |
Rapamycin |
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| MBS3604884-50mg | MyBiosource | 50mg | EUR 195 |
Rapamycin |
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| MBS3604884-5x200mg | MyBiosource | 5x200mg | EUR 760 |
Rapamycin |
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| MBS515013-100mg | MyBiosource | 100mg | EUR 665 |
Rapamycin |
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| MBS515013-20mg | MyBiosource | 20mg | EUR 320 |
Rapamycin |
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| MBS515013-5x100mg | MyBiosource | 5x100mg | EUR 2740 |
Rapamycin, 100mg |
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| R5312 | ACTGene | 100mg | EUR 607.18 |
Rapamycin, 100mg |
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| R5312-100mg | ACTGene | each | EUR 902.4 |
Rapamycin, >99% |
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| BC082-050 | GenDepot | 50mg | EUR 262.8 |
Rapamycin, >99% |
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| BC082-100 | GenDepot | 100mg | EUR 313.2 |
Rapamycin, >99% |
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| BC082-250 | GenDepot | 250mg | EUR 424.8 |
Rapamycin, >99% |
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| BC082-500 | GenDepot | 500mg | EUR 651.6 |
Rapamycin (>85%) |
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| R124000 | Toronto Research Chemicals | 100mg | EUR 80 |
Rapamycin (GMP) |
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| HY-10219G | MedChemExpress | Get quote | Ask for price |
Rapamycin-13C,d3 |
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| HY-10219S1 | MedChemExpress | Get quote | Ask for price |
Seco Rapamycin |
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| HY-19555 | MedChemExpress | 50mg | EUR 2704.8 |
Rapamycin, 10mg |
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| R5312-10mg | ACTGene | each | EUR 244.8 |
Rapamycin-d3 |
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| T12691-10mg | TargetMol Chemicals | 10mg | Ask for price |
Rapamycin-d3 |
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| T12691-1g | TargetMol Chemicals | 1g | Ask for price |
Rapamycin-d3 |
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| T12691-1mg | TargetMol Chemicals | 1mg | Ask for price |
Rapamycin-d3 |
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| T12691-50mg | TargetMol Chemicals | 50mg | Ask for price |
Rapamycin-d3 |
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| T12691-5mg | TargetMol Chemicals | 5mg | Ask for price |
Seco Rapamycin |
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| T38963-10mg | TargetMol Chemicals | 10mg | Ask for price |
Seco Rapamycin |
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| T38963-1g | TargetMol Chemicals | 1g | Ask for price |
Seco Rapamycin |
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| T38963-1mg | TargetMol Chemicals | 1mg | Ask for price |
Seco Rapamycin |
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| T38963-50mg | TargetMol Chemicals | 50mg | Ask for price |
Seco Rapamycin |
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| T38963-5mg | TargetMol Chemicals | 5mg | Ask for price |
Seco Rapamycin |
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| MBS5799522-5mg | MyBiosource | 5(mg | EUR 915 |
Seco Rapamycin |
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| MBS5799522-5x5mg | MyBiosource | 5x5(mg | EUR 3970 |
Rapamycin-d3 |
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| HY-10219S | MedChemExpress | 1 mg | EUR 119.05 |
Sirolimus (Rapamycin) |
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| 100766 | MedKoo Biosciences | 25.0mg | EUR 90 |
Rapamycin (Sirolimus) |
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| E1KS1039 | EnoGene | 50mg | EUR 120 |
Rapamycin (Sirolimus) |
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| A8167-100 | ApexBio | 100 mg | EUR 154 |
Rapamycin (Sirolimus) |
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| A8167-25 | ApexBio | 25 mg | EUR 74 |
Rapamycin (Sirolimus) |
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| A8167-5 | ApexBio | 5 mg | EUR 33 |
Rapamycin (Sirolimus) |
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| A8167-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 48 |
Rapamycin (Sirolimus) |
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| A8167-S | ApexBio | Evaluation Sample | EUR 22 |
Rapamycin (Sirolimus) |
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| MBS8506576-50mg | MyBiosource | 50mg | EUR 210 |
Rapamycin (Sirolimus) |
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| MBS8506576-5x50mg | MyBiosource | 5x50mg | EUR 800 |
- Screening for complement deficiencies within the classical or various complement pathways has primarily been carried out by haemolytic assays. Right here, we describe a easy ELISA-based format for the analysis of three pathways of complement activation. The assays are primarily based on particular coatings for every pathway together with particular buffer methods.
Mouse Mammalian goal of rapamycin ELISA package
- We’ve got standardized these assays and outlined minimize off values to detect complement deficiencies on the completely different ranges of the complement system. The outcomes display the worth of those ELISA-based procedures for the practical evaluation of complement deficiencies in scientific follow. The assay is now obtainable commercially in package kind.
- On this research we examined the timecourse of induction of brain-derived neurotrophic issue (BDNF) mRNA and protein after 1, 3, 5, 7, 14 and 28 days of train within the rat.
- To measure the expression of mRNA for particular person BDNF exons we utilized a semi-quantitative RT–PCR method, whereas BDNF protein was assessed utilizing business ELISA kits. We demonstrated that the gap run by animals elevated considerably (P<0.05) after Four weeks. BDNF protein was considerably (P<0.05) elevated after Four weeks of train, whereas the mRNA for particular person BDNF exons elevated considerably (P<0.05) over the timecourse (exon I after 1 and 28 days and exons II and V after 28 days).
- The Morris water maze was then utilized to display that Three weeks of prior train enhanced the speed of studying on this process. Train, due to this fact, was proven to modulate BDNF induction in a time-dependent method, and this may increasingly translate to enhancements in neurotrophin-mediated duties throughout the CNS.
