Validation and comparability of luminex multiplex cytokine evaluation kits with ELISA: determinations of a panel of 9 cytokines in scientific pattern tradition supernatants.
OBJECTIVE
Analyses of the expression sample of a number of cytokines are incessantly required for characterization of the standing of the immune system because it pertains to Th kind bias and intrinsic ranges of irritation. Classically, evaluation of cytokine expression patterns has been carried out by enzyme-linked immunosorbent assays (ELISA) for every separate analyte.
A brand new expertise, Luminex MAP, facilitates the simultaneous analysis of a number of immune mediators with benefits of upper throughput, smaller pattern quantity, and decrease price. Validation of this expertise has been restricted to small pattern units, restricted use of scientific research specimens, and use of non-commercial reagents.
COLISTIN METHANESULFONATE (SODIUM SALT)
METHODS
Ninety-six specimens from ladies over the course of their respective pregnancies had been evaluated for cytokine concentrations utilizing commercially obtainable ELISA kits and commercially available Luminex MAP kits according to the producers’ instructions. Correlations between information units had been evaluated utilizing Pearson’s correlation coefficient (r).
RESULTS
Wonderful correlations were demonstrated for IL-1 beta, IL-4, IL-5, IL-6, IL-10, IFN gamma, and TNF alpha, in distinction to IL-12 p 70 and IL-13.
COLISTIN SODIUM METHANESULFONATE
CONCLUSIONS
Luminex multiplex expertise has distinct benefits and is a sound various technique to ELISA for the analysis of the vast majority of cytokines examined and for the characterization of immune system standing.

Colistin Methanesulfonate (sodium salt) |
C3046-1000 |
ApexBio |
1 g |
EUR 208.8 |
Colistin Methanesulfonate (sodium salt) |
C3046-5000 |
ApexBio |
5 g |
EUR 734.4 |
Imatinib, Methanesulfonate Salt, >99% |
BC040-101 |
GenDepot |
1g |
EUR 278.4 |
Imatinib, Methanesulfonate Salt, >99% |
BC040-102 |
GenDepot |
2g |
EUR 360 |
Imatinib, Methanesulfonate Salt, >99% |
BC040-105 |
GenDepot |
5g |
EUR 505.2 |
Imatinib, Methanesulfonate Salt, >99% |
BC040-500 |
GenDepot |
500mg |
Ask for price |
Dabrafenib |
D072-10MG |
TOKU-E |
10 mg |
EUR 159.6 |
Dabrafenib |
D072-25MG |
TOKU-E |
25 mg |
EUR 266.4 |
Dabrafenib |
20-abx182412 |
Abbexa |
|
|
|
Dabrafenib (GSK2118436) |
B1407-10 |
ApexBio |
10 mg |
EUR 157.2 |
Dabrafenib (GSK2118436) |
B1407-100 |
ApexBio |
100 mg |
EUR 393.6 |
Dabrafenib (GSK2118436) |
B1407-5.1 |
ApexBio |
10 mM (in 1mL DMSO) |
EUR 135.6 |
Dabrafenib (GSK2118436) |
B1407-50 |
ApexBio |
50 mg |
EUR 268.8 |
Pridinol Methanesulfonate |
A8507-1000 |
ApexBio |
1 g |
EUR 517.2 |
Pridinol Methanesulfonate |
A8507-5.1 |
ApexBio |
10 mM (in 1mL DMSO) |
EUR 129.6 |
Pridinol Methanesulfonate |
A8507-50 |
ApexBio |
50 mg |
EUR 153.6 |
Pridinol Methanesulfonate |
A8507-5000 |
ApexBio |
5 g |
EUR 1198.8 |
Ethyl methanesulfonate |
20-abx186442 |
Abbexa |
-
EUR 678.00
-
EUR 360.00
-
EUR 260.40
|
|
|
Abemaciclib (methanesulfonate) |
HY-16297 |
MedChemExpress |
500mg |
EUR 542.4 |
Dabrafenib Mesylate (GSK-2118436) |
A3347-10 |
ApexBio |
10 mg |
EUR 157.2 |
Dabrafenib Mesylate (GSK-2118436) |
A3347-100 |
ApexBio |
100 mg |
EUR 393.6 |
Dabrafenib Mesylate (GSK-2118436) |
A3347-5.1 |
ApexBio |
10 mM (in 1mL DMSO) |
EUR 150 |
Dabrafenib Mesylate (GSK-2118436) |
A3347-50 |
ApexBio |
50 mg |
EUR 268.8 |
Dabrafenib Mesylate (GSK-2118436) |
A3347-S |
ApexBio |
Evaluation Sample |
EUR 97.2 |
Colistin Sodium Methanesulfonate |
C073-10G |
TOKU-E |
10 g |
EUR 984 |
Colistin Sodium Methanesulfonate |
C073-1G |
TOKU-E |
1 g |
EUR 162 |
Colistin Sodium Methanesulfonate |
C073-5G |
TOKU-E |
5 g |
EUR 528 |
Tariquidar methanesulfonate, hydrate |
A3859-10 |
ApexBio |
10 mg |
EUR 226.8 |
Tariquidar methanesulfonate, hydrate |
A3859-50 |
ApexBio |
50 mg |
EUR 532.8 |
PF-04217903 methanesulfonate |
A3712-100 |
ApexBio |
100 mg |
EUR 714 |
PF-04217903 methanesulfonate |
A3712-25 |
ApexBio |
25 mg |
EUR 351.6 |
PF-04217903 methanesulfonate |
A3712-5 |
ApexBio |
5 mg |
EUR 157.2 |
2-Chloroethyl methanesulfonate |
20-abx183170 |
Abbexa |
|
|
|
Tariquidar (methanesulfonate, hydrate) |
HY-10550A |
MedChemExpress |
100mg |
EUR 734.4 |
Hydroxystilbamidine bis(methanesulfonate) |
HY-108166A |
MedChemExpress |
5mg |
EUR 639.6 |
PF-06424439 (methanesulfonate) |
HY-108341A |
MedChemExpress |
10mM/1mL |
EUR 175.2 |
PF-04217903 (methanesulfonate) |
HY-12017A |
MedChemExpress |
10mM/1mL |
EUR 187.2 |
Ethyl 3-Aminobenzoate (methanesulfonate) |
C3619-100000 |
ApexBio |
100 g |
EUR 381.6 |
Ethyl 3-Aminobenzoate (methanesulfonate) |
C3619-25000 |
ApexBio |
25 g |
EUR 153.6 |
Ethyl 3-Aminobenzoate (methanesulfonate) |
C3619-5.1 |
ApexBio |
10 mM (in 1mL DMSO) |
EUR 135.6 |
Ethyl 3-Aminobenzoate (methanesulfonate) |
C3619-50000 |
ApexBio |
50 g |
EUR 234 |
Ro 31-8220 methanesulfonate |
1946-1 |
Biovision |
each |
EUR 164.4 |
Ro 31-8220 methanesulfonate |
1946-5 |
Biovision |
each |
EUR 457.2 |
CAPS, sodium salt |
CB0097 |
Bio Basic |
50g |
EUR 86.1 |
|
CAPSO, sodium salt |
CD0041 |
Bio Basic |
50g |
EUR 91.32 |
|
Hepes, Sodium Salt |
CH039 |
ABM |
25 g |
EUR 141.6 |
Hepes, Sodium Salt |
CH040 |
ABM |
100 g |
EUR 202.8 |
Hepes, Sodium Salt |
CH041 |
ABM |
500 g |
EUR 448.8 |
Cefamandole sodium salt |
C079-100MG |
TOKU-E |
100 mg |
EUR 177.6 |
Cefamandole sodium salt |
C079-500MG |
TOKU-E |
500 mg |
EUR 568.8 |
Chlorhexidine diacetate salt |
C200-25G |
TOKU-E |
25 g |
EUR 216 |
TAK-700 salt |
A3852-10 |
ApexBio |
10 mg |
EUR 514.8 |
TAK-700 salt |
A3852-100 |
ApexBio |
100 mg |
EUR 2636.4 |
TAK-700 salt |
A3852-25 |
ApexBio |
25 mg |
EUR 774 |
TAK-700 salt |
A3852-5 |
ApexBio |
5 mg |
EUR 315.6 |
Ampicillin, Sodium Salt |
A4004-005 |
GenDepot |
5g |
EUR 111.6 |
Ampicillin, Sodium Salt |
A4004-025 |
GenDepot |
25g |
EUR 206.4 |
Ampicillin, Sodium Salt |
A4004-100 |
GenDepot |
100g |
EUR 370.8 |
DIPSO monosodium salt |
DB0422 |
Bio Basic |
50g |
EUR 99.67 |
|
Dimethylglyoxime disodium salt |
DD1221 |
Bio Basic |
100g |
EUR 76.7 |
|
Diethylcarbamazine citrate salt |
D079-1G |
TOKU-E |
1 g |
EUR 58.8 |
Diethylcarbamazine citrate salt |
D079-5x1G |
TOKU-E |
5 x 1 g |
EUR 141.6 |
Anderson's Basal Salt |
CP001-010 |
GenDepot |
10x1L |
EUR 118.8 |
Purposeful evaluation of the classical, various, and MBL pathways of the complement system: standardization and validation of a easy ELISA.
- Major defence towards invading microorganisms depends upon a practical innate immune system and the complement system performs a serious position in such immunity.
- Deficiencies in one of many parts of the complement system may cause extreme and recurrent infections, systemic ailments, akin to systemic lupus erythematosus (SLE) and renal illness.
Rapamycin |
E1B1842 |
EnoGene |
1mg |
EUR 153.6 |
Rapamycin |
R001-10MG |
TOKU-E |
10 mg |
EUR 243.6 |
Rapamycin |
R001-25MG |
TOKU-E |
25 mg |
EUR 295.2 |
Rapamycin (Sirolimus) |
E1KS1039 |
EnoGene |
50mg |
EUR 247.2 |
Rapamycin (Sirolimus) |
A8167-100 |
ApexBio |
100 mg |
EUR 616.8 |
Rapamycin (Sirolimus) |
A8167-25 |
ApexBio |
25 mg |
EUR 268.8 |
Rapamycin (Sirolimus) |
A8167-5 |
ApexBio |
5 mg |
EUR 129.6 |
Rapamycin (Sirolimus) |
A8167-5.1 |
ApexBio |
10 mM (in 1mL DMSO) |
EUR 351.6 |
Rapamycin (Sirolimus) |
A8167-S |
ApexBio |
Evaluation Sample |
EUR 97.2 |
Rapamycin, >99% |
BC082-050 |
GenDepot |
50mg |
EUR 262.8 |
Rapamycin, >99% |
BC082-100 |
GenDepot |
100mg |
EUR 313.2 |
Rapamycin, >99% |
BC082-250 |
GenDepot |
250mg |
EUR 424.8 |
Rapamycin, >99% |
BC082-500 |
GenDepot |
500mg |
EUR 651.6 |
Rapamycin, 100mg |
R5312-100mg |
ACTGene |
each |
EUR 902.4 |
Rapamycin, 10mg |
R5312-10mg |
ACTGene |
each |
EUR 244.8 |
EZSolution? Rapamycin |
1746-5 |
Biovision |
each |
EUR 326.4 |
Rapamycin (mTOR inhibitor) |
SIH-212-25MG |
Stressmarq |
25 mg |
EUR 490.8 |
|
Rapamycin (mTOR inhibitor) |
SIH-212-5MG |
Stressmarq |
5 mg |
EUR 242.4 |
|
Human Mammalian target of rapamycin ELISA kit |
E01M0019-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Human Mammalian target of rapamycin ELISA kit |
E01M0019-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Human Mammalian target of rapamycin ELISA kit |
E01M0019-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Goat Mammalian target of rapamycin ELISA kit |
E06M0019-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Goat Mammalian target of rapamycin ELISA kit |
E06M0019-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Goat Mammalian target of rapamycin ELISA kit |
E06M0019-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Dog Mammalian target of rapamycin ELISA kit |
E08M0019-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Dog Mammalian target of rapamycin ELISA kit |
E08M0019-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Dog Mammalian target of rapamycin ELISA kit |
E08M0019-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Rat Mammalian target of rapamycin ELISA kit |
E02M0019-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Rat Mammalian target of rapamycin ELISA kit |
E02M0019-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Rat Mammalian target of rapamycin ELISA kit |
E02M0019-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Mouse Mammalian target of rapamycin ELISA kit |
E03M0019-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Mouse Mammalian target of rapamycin ELISA kit |
E03M0019-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Mouse Mammalian target of rapamycin ELISA kit |
E03M0019-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Rabbit Mammalian target of rapamycin ELISA kit |
E04M0019-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Rabbit Mammalian target of rapamycin ELISA kit |
E04M0019-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Rabbit Mammalian target of rapamycin ELISA kit |
E04M0019-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Pig Mammalian target of rapamycin ELISA kit |
E07M0019-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Pig Mammalian target of rapamycin ELISA kit |
E07M0019-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Pig Mammalian target of rapamycin ELISA kit |
E07M0019-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Mechanistic Target of Rapamycin Kinase (mTOR) Antibody |
20-abx133144 |
Abbexa |
-
EUR 360.00
-
EUR 526.80
-
EUR 226.80
|
|
|
Mechanistic Target of Rapamycin Kinase (mTOR) Antibody |
20-abx009248 |
Abbexa |
-
EUR 360.00
-
EUR 526.80
-
EUR 226.80
|
|
|
Mechanistic Target of Rapamycin Kinase (MTOR) Antibody |
abx038215-100ug |
Abbexa |
100 ug |
EUR 469.2 |
|
Mechanistic Target Of Rapamycin Kinase (MTOR) Antibody |
abx331921-100ul |
Abbexa |
100 ul |
EUR 510 |
|
Mechanistic Target Of Rapamycin Kinase (MTOR) Antibody |
abx331979-100ul |
Abbexa |
100 ul |
EUR 510 |
|
Monkey Mammalian target of rapamycin ELISA kit |
E09M0019-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Monkey Mammalian target of rapamycin ELISA kit |
E09M0019-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Monkey Mammalian target of rapamycin ELISA kit |
E09M0019-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Guinea pig Mammalian target of rapamycin ELISA kit |
E05M0019-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Guinea pig Mammalian target of rapamycin ELISA kit |
E05M0019-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Guinea pig Mammalian target of rapamycin ELISA kit |
E05M0019-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Rat mammalian target of rapamycin(mTOR)ELISA Kit |
GA-E0434RT-48T |
GenAsia Biotech |
48T |
EUR 380.4 |
Rat mammalian target of rapamycin(mTOR)ELISA Kit |
GA-E0434RT-96T |
GenAsia Biotech |
96T |
EUR 595.2 |
Target of Rapamycin Complex Subunit LST8 (MLST8) Antibody |
abx117025-100ug |
Abbexa |
100 ug |
EUR 560.4 |
|
Target of Rapamycin Complex Subunit LST8 (MLST8) Antibody |
20-abx000982 |
Abbexa |
-
EUR 493.20
-
EUR 710.40
-
EUR 218.40
-
EUR 376.80
|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
Human Mammalian Target of Rapamycin (mTOR) CLIA Kit |
abx195664-96tests |
Abbexa |
96 tests |
EUR 990 |
|
Target of rapamycin complex subunit LST8 (MLST8) Antibody |
20-abx333910 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Human Mammalian Target of Rapamycin(mTOR)ELISA Kit |
YLA1299HU-48T |
Shanghai YL Biotech |
48T |
EUR 435 |
Human Mammalian Target of Rapamycin(mTOR)ELISA Kit |
YLA1299HU-96T |
Shanghai YL Biotech |
96T |
EUR 562.5 |
Mouse Mammalian Target of Rapamycin(mTOR)ELISA Kit |
YLA1811MO-48T |
Shanghai YL Biotech |
48T |
EUR 465 |
Mouse Mammalian Target of Rapamycin(mTOR)ELISA Kit |
YLA1811MO-96T |
Shanghai YL Biotech |
96T |
EUR 600 |
ELISA kit for Human mTOR (Mammalian Target of Rapamycin) |
E-EL-H1655 |
Elabscience Biotech |
1 plate of 96 wells |
EUR 640.8 |
|
Human Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) |
1-CSB-EP857767HU |
Cusabio |
-
EUR 456.00
-
EUR 256.80
-
EUR 1570.80
-
EUR 672.00
-
EUR 1047.60
-
EUR 314.40
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
CLIA kit for Human mTOR (Mammalian Target of Rapamycin) |
E-CL-H1050 |
Elabscience Biotech |
1 plate of 96 wells |
EUR 700.8 |
|
FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) Antibody |
20-abx132395 |
Abbexa |
-
EUR 393.60
-
EUR 978.00
-
EUR 510.00
-
EUR 184.80
-
EUR 309.60
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) Antibody |
20-abx128810 |
Abbexa |
-
EUR 493.20
-
EUR 159.60
-
EUR 1378.80
-
EUR 678.00
-
EUR 376.80
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Human Rapamycin-Insensitive Companion Of MTOR (RICTOR) ELISA Kit |
abx382812-96tests |
Abbexa |
96 tests |
EUR 1093.2 |
|
Target of rapamycin complex subunit LST8 (MLST8) Antibody (HRP) |
20-abx337874 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Target of rapamycin complex subunit LST8 (MLST8) Antibody (FITC) |
20-abx337875 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Target of rapamycin complex subunit LST8 (MLST8) Antibody (Biotin) |
20-abx337876 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Mouse Rapamycin- insensitive companion of mTOR, Rictor ELISA KIT |
ELI-41325m |
Lifescience Market |
96 Tests |
EUR 1038 |
Human Rapamycin- insensitive companion of mTOR, RICTOR ELISA KIT |
ELI-18108h |
Lifescience Market |
96 Tests |
EUR 988.8 |
Recombinant FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) |
4-RPB806Hu01 |
Cloud-Clone |
-
EUR 603.84
-
EUR 285.60
-
EUR 1934.40
-
EUR 724.80
-
EUR 1329.60
-
EUR 480.00
-
EUR 4656.00
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Human Rapamycin insensitive companion of mTOR(RICTOR)ELISA Kit |
YLA1128HU-48T |
Shanghai YL Biotech |
48T |
EUR 435 |
- Screening for complement deficiencies within the classical or various complement pathways has primarily been carried out by haemolytic assays. Right here, we describe a easy ELISA-based format for the analysis of three pathways of complement activation. The assays are primarily based on particular coatings for every pathway together with particular buffer methods.
Mouse Mammalian goal of rapamycin ELISA package
- We’ve got standardized these assays and outlined minimize off values to detect complement deficiencies on the completely different ranges of the complement system. The outcomes display the worth of those ELISA-based procedures for the practical evaluation of complement deficiencies in scientific follow. The assay is now obtainable commercially in package kind.
- On this research we examined the timecourse of induction of brain-derived neurotrophic issue (BDNF) mRNA and protein after 1, 3, 5, 7, 14 and 28 days of train within the rat.
- To measure the expression of mRNA for particular person BDNF exons we utilized a semi-quantitative RT–PCR method, whereas BDNF protein was assessed utilizing business ELISA kits. We demonstrated that the gap run by animals elevated considerably (P<0.05) after Four weeks. BDNF protein was considerably (P<0.05) elevated after Four weeks of train, whereas the mRNA for particular person BDNF exons elevated considerably (P<0.05) over the timecourse (exon I after 1 and 28 days and exons II and V after 28 days).
- The Morris water maze was then utilized to display that Three weeks of prior train enhanced the speed of studying on this process. Train, due to this fact, was proven to modulate BDNF induction in a time-dependent method, and this may increasingly translate to enhancements in neurotrophin-mediated duties throughout the CNS.