Interaction Between Recombinant I-domain from Integrin alpha 2

Measurement of the Interplay Between Recombinant I-domain from Integrin alpha 2 beta 1 and a Triple Helical Collagen Peptide with the GFOGER Binding Motif Utilizing Molecular Power Spectroscopy.

 

 

The position of the collagen-platelet interplay is of essential significance to the haemostatic response throughout each harm and pathogenesis of the blood vessel wall.

 

Of explicit curiosity is the excessive affinity interplay of the platelet transmembrane receptor, alpha 2 beta 1, accountable for agency attachment of platelets to collagen at and round harm websites.

 

We make use of single molecule power spectroscopy (SMFS) utilizing the atomic power microscope (AFM) to review the interplay of the I-domain from integrin alpha 2 beta 1 with an artificial collagen associated triple-helical peptide containing the high-affinity integrin-binding GFOGER motif, and a management peptide missing this sequence, known as GPP.

Human Integrin alpha- X, ITGAX ELISA KIT

By utilising artificial peptides on this method we’re in a position to research on the molecular stage subtleties that will in any other case be misplaced when contemplating cell-to-collagen matrix interactions utilizing ensemble strategies.

 

We display for the primary time the complexity of this interplay as illustrated by the advanced multi-peaked power spectra and verify specificity utilizing management blocking experiments. As well as we observe particular interplay of the GPP peptide sequence with the I-domain. We suggest a mannequin to clarify these observations.

Integrin Alpha X (CD11c) Antibody (PE)

 

Alpha V Beta 5 Integrin, Peptide Aptamer, FITC labelled

AP-301-F 1 mg Ask for price

Human Integrin alpha-X ELISA kit

E01I0494-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Human Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Integrin alpha-X ELISA kit

E01I0494-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Human Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Integrin alpha-X ELISA kit

E01I0494-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Human Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

anti- CD11c/Integrin alpha X antibody

FNab01412 100µg
EUR 606.3
Description: Antibody raised against CD11c/Integrin alpha X

anti- CD11c/Integrin alpha X antibody

FNab01413 100µg
EUR 658.5
Description: Antibody raised against CD11c/Integrin alpha X

Anti-CD11c/Integrin alpha X antibody

PAab01412 100 ug
EUR 426

Integrin alpha X antibody

70R-12673 100 ul
EUR 550
Description: Affinity purified Rabbit polyclonal Integrin alpha X antibody

Integrin alpha X Antibody

GWB-5111F7 0.1 mg Ask for price

Human Integrin Alpha X (ITGaX) Protein

20-abx166474
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  • 100 ug
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Rat Integrin alpha-X

E01A14183 96T
EUR 700
Description: ELISA

Human Integrin, alpha X (CD11C) Elisa Kit

EK713871 96 Wells
EUR 0.4

Human Integrin Alpha X (CD11c) ELISA Kit

EKN53617-48T 48T
EUR 382.2

Human Integrin Alpha X (CD11c) ELISA Kit

EKN53617-5x96T 5x96T
EUR 2593.5

Human Integrin Alpha X (CD11c) ELISA Kit

EKN53617-96T 96T
EUR 546

Human Integrin alpha- X, ITGAX ELISA KIT

ELI-13625h 96 Tests
EUR 988.8

Human Integrin Alpha X (CD11c) ELISA Kit

EKU12505-48T 48T
EUR 563.5

Human Integrin Alpha X (CD11c) ELISA Kit

EKU12505-5x96T 5x96T
EUR 3823.75

Human Integrin Alpha X (CD11c) ELISA Kit

EKU12505-96T 96T
EUR 805

Goat Integrin alpha-X

E01A49092 96T
EUR 700
Description: ELISA

Human Integrin alpha-X (ITGAX) ELISA Kit

RK11290 96T
EUR 280

Integrin Alpha X (ITGaX) Polyclonal Antibody (Human)

4-PAB159Hu01
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  • 100ul
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Description: A Rabbit polyclonal antibody against Human Integrin Alpha X (ITGaX)

Sheep Integrin alpha-X

E01A101385 96T
EUR 700
Description: ELISA

Mouse Integrin alpha-X

E01A22925 96T
EUR 700
Description: ELISA

CD11c/Integrin alpha X ELISA KIT|Human

EF008489 96 Tests
EUR 826.8

Rabbit Integrin alpha-X

E01A31659 96T
EUR 700
Description: ELISA

Canine Integrin alpha-X

E01A66522 96T
EUR 700
Description: ELISA

Bovine Integrin alpha-X

E01A83957 96T
EUR 700
Description: ELISA

Monkey Integrin alpha-X

E01A75239 96T
EUR 700
Description: ELISA

Human ITGAX / Integrin alpha-X ELISA Kit

E9095h 96T
EUR 736

Porcine Integrin alpha-X

E01A57810 96T
EUR 700
Description: ELISA

Chicken Integrin alpha-X

E01A92677 96T
EUR 700
Description: ELISA

Human Integrin Alpha X / CD11C (ITGAX) Protein

abx166474-100g 100 µg
EUR 525

Human Integrin Alpha X / CD11C (ITGAX) Protein

abx166474-10g 10 µg
EUR 212.5

Human Integrin Alpha X / CD11C (ITGAX) Protein

abx166474-50g 50 µg
EUR 362.5

Integrin Alpha X (ITGAX) Antibody

20-abx113189
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  • 150 ul
  • 50 ul

Integrin Alpha X (ITGaX) Antibody

20-abx104960
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  • 100 ug
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  • 200 ug
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Integrin Alpha X (ITGaX) Antibody

20-abx129411
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  • 100 ug
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Integrin Alpha X (ITGAX) Antibody

20-abx124171
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  • 100 ul
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Integrin Alpha X (CD11c) Antibody

abx139631-01mg 0.1 mg
EUR 444

Integrin Alpha X (ITGAX) Antibody

abx033098-400ul 400 ul
EUR 627.6

Integrin Alpha X (ITGAX) Antibody

abx033098-80l 80 µl
EUR 343.2

Integrin Alpha X (CD11c) Antibody

abx200116-100ug 100 ug
EUR 477.6

Integrin Alpha X (CD11c) Antibody

20-abx200679
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  • 100 ug
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Integrin Alpha X (CD11c) Antibody

abx216334-100ug 100 ug
EUR 526.8

Integrin Alpha X (ITGAX) Antibody

abx330439-100ul 100 ul
EUR 510

Integrin Alpha X (CD11c) Antibody

abx411892-50ug 50 ug
EUR 811.2

Integrin Alpha X (CD11c) Antibody

abx413004-025mg 0.25 mg
EUR 678

Integrin Alpha X (CD11c) Antibody

abx413008-01mg 0.1 mg
EUR 526.8

Integrin Alpha X (CD11c) Antibody

abx413011-25ug 25 ug
EUR 326.4

Integrin Alpha X (CD11c) Antibody

abx413356-2ml 2 ml
EUR 727.2

Integrin Alpha X (CD11c) Antibody

abx413688-02mg 0.2 mg
EUR 678

Integrin Alpha X (CD11c) Antibody

abx413691-01mg 0.1 mg
EUR 526.8

Integrin Alpha X (CD11c) Antibody

abx413694-25ug 25 ug
EUR 326.4

Integrin Alpha X (CD11c) Antibody

abx414593-01mg 0.1 mg
EUR 910.8

Integrin Alpha X (CD11c) Antibody

abx415362-01mg 0.1 mg
EUR 543.6

Integrin Alpha X (ITGAX) Antibody

20-abx322638
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  • 100 ul
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Integrin Alpha X (ITGAX) Antibody

20-abx339188
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  • 100 ul
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Integrin Alpha X (ITGAX) Antibody

20-abx339189
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  • 100 ul
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CD11c/Integrin alpha X antibody

CAF50517-100ug 100ug
EUR 312

Mouse Monoclonal anti-human Integrin alpha X (CD11c)

hAP-0422 100ug
EUR 250

Integrin alpha X Antibody (Biotin)

GWB-E5793A 0.1 mg Ask for price

Integrin Alpha X (ITGaX) Polyclonal Antibody (Human), PE

4-PAB159Hu01-PE
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  • 100ul
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Description: A Rabbit polyclonal antibody against Human Integrin Alpha X (ITGaX). This antibody is labeled with PE.

Integrin, Alpha X (Antigen CD11C (P150), Alpha Polypeptide) (ITGAX), Human

GWB-36F42C 0.05 mg Ask for price

Integrin, Alpha X (Antigen CD11C (P150), Alpha Polypeptide) (ITGAX), Human

GWB-570204 0.05 mg Ask for price

Integrin Alpha X (ITGaX) Polyclonal Antibody (Human), APC

4-PAB159Hu01-APC
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  • 100ul
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Description: A Rabbit polyclonal antibody against Human Integrin Alpha X (ITGaX). This antibody is labeled with APC.

Integrin Alpha X (ITGaX) Polyclonal Antibody (Human), Cy3

4-PAB159Hu01-Cy3
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  • 100ul
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Description: A Rabbit polyclonal antibody against Human Integrin Alpha X (ITGaX). This antibody is labeled with Cy3.

Integrin Alpha X (ITGaX) Polyclonal Antibody (Human), HRP

4-PAB159Hu01-HRP
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  • 100ul
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Description: A Rabbit polyclonal antibody against Human Integrin Alpha X (ITGaX). This antibody is labeled with HRP.

Human Integrin Alpha X / CD11C (ITGAX) ELISA Kit

abx259467-96tests 96 tests
EUR 1093.2

Human Integrin Alpha X / CD11C (ITGAX) ELISA Kit

abx259467-1mg 1 mg
EUR 6187.5

Human Integrin Alpha X / CD11C (ITGAX) ELISA Kit

abx259467-20g 20 µg
EUR 3275

Human Integrin Alpha X / CD11C (ITGAX) ELISA Kit

abx259467-5g 5 µg
EUR 687.5

Integrin Alpha X (ITGaX) Polyclonal Antibody (Human), FITC

4-PAB159Hu01-FITC
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  • 100ul
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Description: A Rabbit polyclonal antibody against Human Integrin Alpha X (ITGaX). This antibody is labeled with FITC.

Integrin alpha X Rabbit mAb

E2R380832 100ul
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Guinea Pig Integrin alpha-X

E01A40373 96T
EUR 700
Description: ELISA

Integrin Alpha X (CD11c) Antibody (PE)

abx139633-100tests 100 tests
EUR 577.2

Integrin Alpha X (CD11c) Antibody (PE)

abx200118-100tests 100 tests
EUR 794.4

Integrin Alpha X (CD11c) Antibody (PE)

20-abx200676
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  • 100 ug
  • 25 ug

Active Integrin Alpha X (CD11c)

APB159Hu61 10ug
EUR 212

Mouse Integrin alpha-X (Itgax)

1-CSB-BP874106MO1
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Description: Recombinant Mouse Integrin alpha-X(Itgax),partial expressed in Baculovirus

Integrin Alpha X (CD11c) Antibody (APC)

abx139634-100tests 100 tests
EUR 577.2

Integrin Alpha X (CD11c) Antibody (APC)

abx200119-100tests 100 tests
EUR 1178.4

Integrin Alpha X (CD11c) Antibody (APC)

20-abx200672
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  • 100 ug
  • 25 ug

Integrin Alpha X (CD11c) Antibody (RPE)

abx413009-100tests 100 tests
EUR 710.4

Integrin Alpha X (CD11c) Antibody (RPE)

abx413010-25tests 25 tests
EUR 376.8

Integrin Alpha X (CD11c) Antibody (RPE)

abx413692-100tests 100 tests
EUR 710.4

Integrin Alpha X (CD11c) Antibody (RPE)

abx413693-25tests025ml 25 tests (0.25 ml)
EUR 376.8

Integrin Alpha X (CD11c) Antibody (FITC)

abx139635-100tests 100 tests
EUR 510

Integrin Alpha X (CD11c) Antibody (FITC)

abx200117-100tests 100 tests
EUR 477.6

 

Recombinant expression in human cells of lively integrin alpha 1 beta 1-blocking RTS-disintegrin jerdostatin.

  • Jerdostatin, an RTS quick disintegrin cloned from Protobothrops jerdonii and recombinantly produced in Escherichia coli, is a potent and particular antagonist of the alpha(1)beta(1) integrin. Jerdostatin selectively blocked the adhesion of alpha(1)beta(1)-Ok562 cell to collagens I and IV in vitro and angiogenesis in vivo.

 

  • Right here we report the recombinant manufacturing of jerdostatin in a mammalian cell system, a prerequisite for growing a conditional transgenic mouse to research the impact of systemic expression of jerdostatin on tumor growth. For correct export of jerdostatin, a secretion chief sequence was engineered on the protein’s N-terminus.

Mouse Integrin alpha-X ELISA equipment

  • A FLAG epitope was additionally included on the N-terminus of the mature disintegrin to facilitate its isolation and characterization of recombinant jerdostatin (rJerd).

 

  • This pRc-CMV/FLAG-rJerd assemble was transiently expressed in HEK-293 cells and was effectively secreted into the tradition medium. rJerd sure to recombinant soluble alpha(1)beta(1) integrin in a saturable and cation-independent method. Soluble rJerd additionally inhibited the binding of alpha(1)beta(1) integrin to the CB3 fragment of collagen IV in a dose-dependent method (IC(50) 570 nM). Mammalian cell-expressed jerdostatin disrupted the adhesion of RuGli cells to collagen IV.

 

  • Our outcomes spotlight pRc-CMV/FLAG-rJerd as an appropriate assemble for expressing soluble lively alpha(1)beta(1)-blocking jerdostatin in a mammalian cell system.

 

OBJECTIVE
To ascertain human integrin-alpha v adenovirus expressed in human liver most cancers SMMC-7721 cells and analyze the traits of integrin-alpha adhesion to liver most cancers cells.
METHODS
Human integrin-alpha v cDNA was cloned into the plasmid pAdTrack-CMV. Then, pAdTrack-integrin-alpha v was cotransformed with adenoviral spine vector into E. coli pressure BJ5183. The recombinant adenoviral plasmid was recognized by restriction evaluation with Pac I and transfected into HEK-293A cells to package deal and amplify recombinant adenovirus particles. Western blot confirmed that integrin-alpha v gene was precisely transcript and expressed in SMMC-

CFDA SE Cell Tracer Kit

K1023-1 1 kit
EUR 96
Description: The CFDA SE (carboxyfluorescein diacetate succinimidyl ester) Cell Tracer Kit is usually used in fluorescence analyses as a long-term tracer of cells. The Kit can be used for in vitro and in vivo labeling of cells to determine whether or not a cell is proliferating, especially.

Yellow 101

T35247-10mg 10mg Ask for price
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Yellow 101

T35247-1g 1g Ask for price
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Yellow 101

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Yellow 101

T35247-50mg 50mg Ask for price
Description: Yellow 101

Yellow 101

T35247-5mg 5mg Ask for price
Description: Yellow 101

Bolle Tracker PSJ Yellow Lens - EACH

SAF1912 EACH
EUR 23.36

Cell Strainer 100um Yellow Sterile Ind. Wrapped - PK50

ARG1222 PK50
EUR 253.8

Fluorol Yellow 088

F621725 1g
EUR 150
Description: 81-37-8

Pigment Yellow 180

P437800 10g
EUR 82
Description: 77804-81-0

Methyl Yellow

12814-64 10G
EUR 32.2

MitoPB Yellow

D123550 25mg
EUR 15000

Methyl Yellow

M30640 25G
EUR 138.73

Alcian yellow

T29841-10mg 10mg Ask for price
Description: Alcian yellow

Alcian yellow

T29841-1g 1g Ask for price
Description: Alcian yellow

Alcian yellow

T29841-1mg 1mg Ask for price
Description: Alcian yellow

Alcian yellow

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Description: Alcian yellow

Alcian yellow

T29841-5mg 5mg Ask for price
Description: Alcian yellow

Yellow colour

PCT1315-250G 1 unit
EUR 60.87
Description: Yellow colour

Sunset yellow

GRM10357-25G 1 unit
EUR 10.08
Description: Sunset yellow

mFluor™ Yellow 630 SE

1170 1 mg
EUR 60

Oxazole yellow

341177 1.0mg
EUR 390

Metanil Yellow

M321778 1g
EUR 76
Description: 587-98-4

Oxazole yellow

T33840-10mg 10mg Ask for price
Description: Oxazole yellow

Oxazole yellow

T33840-1g 1g Ask for price
Description: Oxazole yellow

Oxazole yellow

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Lucifer yellow

T32926-10mg 10mg Ask for price
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Lucifer yellow

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Description: Lucifer yellow

Dimethyl Yellow

D473790 10g
EUR 178
Description: 60-11-7

Acridine Yellow

T29619-10mg 10mg Ask for price
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Acridine Yellow

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Alcian Yellow (C.I. 12840)

GT4246-1 1
EUR 102.7

Alcian Yellow (C.I. 12840)

GT4246-1G 1 g
EUR 160.8

mFluor™ Yellow 630 acid

1145 5 mg
EUR 60

Safflower yellow

T19056-10mg 10mg Ask for price
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Safflower yellow

T19056-1g 1g Ask for price
Description: Safflower yellow

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Quinoline Yellow

Q701040 10g
EUR 86
Description: 8003-22-3

Safflower yellow

HY-N0938 50mg
EUR 159.6

Quinoline yellow

RM877-25G 1 unit
EUR 27.08
Description: Quinoline yellow

Quinoline yellow

RM877-5G 1 unit
EUR 6.93
Description: Quinoline yellow

Metanil Yellow (C.I. 13065)

GT1665-100 100
EUR 39.4

Metanil Yellow (C.I. 13065)

GT1665-100G 100 g
EUR 84

Metanil Yellow (C.I. 13065)

GT1665-250 250
EUR 79.1

Metanil Yellow (C.I. 13065)

GT1665-250G 250 g
EUR 132

Metanil Yellow (C.I. 13065)

GT1665-50 50
EUR 23.8

Metanil Yellow (C.I. 13065)

GT1665-50G 50 g
EUR 64.8

Martius Yellow (C.I. 10315)

GL3107-100 100
EUR 166

Martius Yellow (C.I. 10315)

GL3107-100G 100 g
EUR 237.6

Martius Yellow (C.I. 10315)

GL3107-25 25
EUR 59.3

Martius Yellow (C.I. 10315)

GL3107-25G 25 g
EUR 108

C.I.Vat Yellow 2

20-abx181248
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Vat Yellow 13

T35039-10mg 10mg Ask for price
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Vat Yellow 13

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Vat Yellow 13

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Vat Yellow 13

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Oil Yellow D

O02562 10G
EUR 199.43

Vat Yellow 4

V099300 50mg
EUR 1200
Description: 128-66-5

Metachrome yellow

596178 25.0g
EUR 220

Metachrome yellow

T33300-10mg 10mg Ask for price
Description: Metachrome yellow

Metachrome yellow

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Description: Metachrome yellow

Metachrome yellow

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Metachrome yellow

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Description: Metachrome yellow

Integrin Alpha X (CD11c) Antibody (PerCP)

7721 cells. Twenty 4 hours after transfection, the impact of integrin-alpha on adhesion of SMMC-7721 cells was detected by adhesion experiment.
RESULTS
Recombinant adenovirus vector of integrin-alpha v gene was efficiently constructed and excessive titers of recombinant adenovirus was obtained. Adhesion cell rely was considerably greater in integrin-alpha v transfected cells than in untransfected and Advert-null-transfected cells (P<0.01).
CONCLUSIONS
Over expression of integrin-alpha v promotes adhesion of SMMC-7721 cells, is a crucial molecular mechanism of tumor metastasis.

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