Nicholas

Interaction Between Recombinant I-domain from Integrin alpha 2

Measurement of the Interplay Between Recombinant I-domain from Integrin alpha 2 beta 1 and a Triple Helical Collagen Peptide with the GFOGER Binding Motif Utilizing Molecular Power Spectroscopy.

 

 

The position of the collagen-platelet interplay is of essential significance to the haemostatic response throughout each harm and pathogenesis of the blood vessel wall.

 

Of explicit curiosity is the excessive affinity interplay of the platelet transmembrane receptor, alpha 2 beta 1, accountable for agency attachment of platelets to collagen at and round harm websites.

 

We make use of single molecule power spectroscopy (SMFS) utilizing the atomic power microscope (AFM) to review the interplay of the I-domain from integrin alpha 2 beta 1 with an artificial collagen associated triple-helical peptide containing the high-affinity integrin-binding GFOGER motif, and a management peptide missing this sequence, known as GPP.

Human Integrin alpha- X, ITGAX ELISA KIT

By utilising artificial peptides on this method we’re in a position to research on the molecular stage subtleties that will in any other case be misplaced when contemplating cell-to-collagen matrix interactions utilizing ensemble strategies.

 

We display for the primary time the complexity of this interplay as illustrated by the advanced multi-peaked power spectra and verify specificity utilizing management blocking experiments. As well as we observe particular interplay of the GPP peptide sequence with the I-domain. We suggest a mannequin to clarify these observations.

Integrin Alpha X (CD11c) Antibody (PE)

 

Human Integrin alpha-X ELISA kit
E01I0494-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Human Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Integrin alpha-X ELISA kit
E01I0494-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Human Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Integrin alpha-X ELISA kit
E01I0494-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Human Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Integrin alpha-X ELISA Kit
MBS7254267-10x96StripWells 10x96-Strip-Wells
EUR 5685

Human Integrin alpha-X ELISA Kit
MBS7254267-48StripWells 48-Strip-Wells
EUR 485

Human Integrin alpha-X ELISA Kit
MBS7254267-5x96StripWells 5x96-Strip-Wells
EUR 3020

Human Integrin alpha-X ELISA Kit
MBS7254267-96StripWells 96-Strip-Wells
EUR 690

Alpha V Beta 5 Integrin, Peptide Aptamer, FITC labelled
AP-301-F 1 mg Ask for price

Human Integrin Alpha X (ITGaX) Protein
20-abx166474
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

anti- CD11c/Integrin alpha X antibody
FNab01412 100µg
EUR 606.3
Description: Antibody raised against CD11c/Integrin alpha X

anti- CD11c/Integrin alpha X antibody
FNab01413 100µg
EUR 658.5
Description: Antibody raised against CD11c/Integrin alpha X

Anti-CD11c/Integrin alpha X antibody
PAab01412 100ug
EUR 245

Anti-CD11c/Integrin alpha X antibody
PAab01413 100ug
EUR 275

Integrin Alpha X (ITGaX) Polyclonal Antibody (Human)
4-PAB159Hu01
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Integrin Alpha X (ITGaX)

Human Integrin, alpha X (CD11C) Elisa Kit
EK713871 96 Wells
EUR 0.4

Human Integrin Alpha X (CD11c) ELISA Kit
EKN53617-48T 48T
EUR 382.2

Human Integrin Alpha X (CD11c) ELISA Kit
EKN53617-5x96T 5x96T
EUR 2593.5

Human Integrin Alpha X (CD11c) ELISA Kit
EKN53617-96T 96T
EUR 546

Human Integrin Alpha X (CD11c) ELISA Kit
EKU12505-48T 48T
EUR 563.5

Human Integrin Alpha X (CD11c) ELISA Kit
EKU12505-5x96T 5x96T
EUR 3823.75

Human Integrin Alpha X (CD11c) ELISA Kit
EKU12505-96T 96T
EUR 805

Human Integrin alpha- X, ITGAX ELISA KIT
ELI-13625h 96tests
EUR 696

Human Integrin Alpha X (CD11c) ELISA Kit
MBS2127238-10x96StripWells 10x96-Strip-Wells
EUR 5320

Human Integrin Alpha X (CD11c) ELISA Kit
MBS2127238-24StripWells 24-Strip-Wells
EUR 360

Human Integrin Alpha X (CD11c) ELISA Kit
MBS2127238-48StripWells 48-Strip-Wells
EUR 545

Human Integrin Alpha X (CD11c) ELISA Kit
MBS2127238-5x96StripWells 5x96-Strip-Wells
EUR 2915

Human Integrin Alpha X (CD11c) ELISA Kit
MBS2127238-96StripWells 96-Strip-Wells
EUR 720

Human Integrin Alpha X (CD11c) ELISA Kit
MBS2709875-10x96StripWells 10x96-Strip-Wells
EUR 3130

Human Integrin Alpha X (CD11c) ELISA Kit
MBS2709875-24StripWells 24-Strip-Wells
EUR 255

Human Integrin Alpha X (CD11c) ELISA Kit
MBS2709875-48StripWells 48-Strip-Wells
EUR 330

Human Integrin Alpha X (CD11c) ELISA Kit
MBS2709875-5x96StripWells 5x96-Strip-Wells
EUR 1630

Human Integrin Alpha X (CD11c) ELISA Kit
MBS2709875-96StripWells 96-Strip-Wells
EUR 420

Human Integrin Alpha X (ITGAX) ELISA Kit
MBS9940475-10x96StripWells 10x96-Strip-Wells
EUR 6725

Human Integrin Alpha X (ITGAX) ELISA Kit
MBS9940475-48StripWells 48-Strip-Wells
EUR 550

Human Integrin Alpha X (ITGAX) ELISA Kit
MBS9940475-5x96StripWells 5x96-Strip-Wells
EUR 3420

Human Integrin Alpha X (ITGAX) ELISA Kit
MBS9940475-96StripWells 96-Strip-Wells
EUR 765

Human Integrin alpha-X (ITGAX) ELISA Kit
RK11290 96T
EUR 280

Human Integrin alpha-X, ITGAX ELISA Kit
MBS9324714-10x96StripWells 10x96-Strip-Wells
EUR 6725

Human Integrin alpha-X, ITGAX ELISA Kit
MBS9324714-48StripWells 48-Strip-Wells
EUR 550

Human Integrin alpha-X, ITGAX ELISA Kit
MBS9324714-5x96StripWells 5x96-Strip-Wells
EUR 3420

Human Integrin alpha-X, ITGAX ELISA Kit
MBS9324714-96StripWells 96-Strip-Wells
EUR 765

Integrin alpha X antibody
70R-12673 100 ul
EUR 550
Description: Affinity purified Rabbit polyclonal Integrin alpha X antibody

Integrin alpha X Antibody
GWB-5111F7 0.1 mg Ask for price

Integrin alpha X antibody
MBS835754-01mL 0.1mL
EUR 1070

Integrin alpha X antibody
MBS835754-5x01mL 5x0.1mL
EUR 4655

Human CD11c/Integrin alpha X ELISA KIT
EF008489 96tests
EUR 566

Human Integrin Alpha X / CD11C (ITGAX) Protein
abx166474-100g 100 µg
EUR 525

Human Integrin Alpha X / CD11C (ITGAX) Protein
abx166474-10g 10 µg
EUR 212.5

Human Integrin Alpha X / CD11C (ITGAX) Protein
abx166474-50g 50 µg
EUR 362.5

Human ITGAX / Integrin alpha-X ELISA Kit
E9095h 96T
EUR 736

Human ITGAX/Integrin alpha-X ELISA Kit
MBS2905103-10x96StripWells 10x96-Strip-Wells
EUR 4080

Human ITGAX/Integrin alpha-X ELISA Kit
MBS2905103-48StripWells 48-Strip-Wells
EUR 390

Human ITGAX/Integrin alpha-X ELISA Kit
MBS2905103-5x96StripWells 5x96-Strip-Wells
EUR 2220

Human ITGAX/Integrin alpha-X ELISA Kit
MBS2905103-96StripWells 96-Strip-Wells
EUR 520

Integrin Alpha X (ITGaX) Polyclonal Antibody (Human), PE
4-PAB159Hu01-PE
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Integrin Alpha X (ITGaX). This antibody is labeled with PE.

Integrin, Alpha X (Antigen CD11C (P150), Alpha Polypeptide) (ITGAX), Human
GWB-36F42C 0.05 mg Ask for price

Integrin, Alpha X (Antigen CD11C (P150), Alpha Polypeptide) (ITGAX), Human
GWB-570204 0.05 mg Ask for price

Recombinant Human Integrin alpha-X (ITGAX) , partial
MBS948167-005mgBaculovirus 0.05mg(Baculovirus)
EUR 1020

Recombinant Human Integrin alpha-X (ITGAX) , partial
MBS948167-005mgEColi 0.05mg(E-Coli)
EUR 690

Recombinant Human Integrin alpha-X (ITGAX) , partial
MBS948167-005mgYeast 0.05mg(Yeast)
EUR 850

Recombinant Human Integrin alpha-X (ITGAX) , partial
MBS948167-02mgEColi 0.2mg(E-Coli)
EUR 890

Recombinant Human Integrin alpha-X (ITGAX) , partial
MBS948167-05mgEColi 0.5mg(E-Coli)
EUR 980

Integrin Alpha X (ITGaX) Polyclonal Antibody (Human), APC
4-PAB159Hu01-APC
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Integrin Alpha X (ITGaX). This antibody is labeled with APC.

Integrin Alpha X (ITGaX) Polyclonal Antibody (Human), Cy3
4-PAB159Hu01-Cy3
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Integrin Alpha X (ITGaX). This antibody is labeled with Cy3.

Integrin Alpha X (ITGaX) Polyclonal Antibody (Human), HRP
4-PAB159Hu01-HRP
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Integrin Alpha X (ITGaX). This antibody is labeled with HRP.

Mouse Monoclonal anti-human Integrin alpha X (CD11c)
hAP-0422 100ug
EUR 250

Integrin Alpha X (ITGaX) Polyclonal Antibody (Human), FITC
4-PAB159Hu01-FITC
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
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Description: A Rabbit polyclonal antibody against Human Integrin Alpha X (ITGaX). This antibody is labeled with FITC.

Human Integrin Alpha X / CD11C (ITGAX) ELISA Kit
abx259467-96tests 96 tests
EUR 1093.2

Human Integrin Alpha X / CD11C (ITGAX) ELISA Kit
abx259467-1mg 1 mg
EUR 6187.5

Human Integrin Alpha X / CD11C (ITGAX) ELISA Kit
abx259467-20g 20 µg
EUR 3275

Human Integrin Alpha X / CD11C (ITGAX) ELISA Kit
abx259467-5g 5 µg
EUR 687.5

Rat Integrin alpha-X
E01A14183 96T
EUR 700
Description: ELISA

Goat Integrin alpha-X
E01A49092 96T
EUR 700
Description: ELISA

Sheep Integrin alpha-X
E01A101385 96T
EUR 700
Description: ELISA

Mouse Integrin alpha-X
E01A22925 96T
EUR 700
Description: ELISA

Rabbit Integrin alpha-X
E01A31659 96T
EUR 700
Description: ELISA

Canine Integrin alpha-X
E01A66522 96T
EUR 700
Description: ELISA

Bovine Integrin alpha-X
E01A83957 96T
EUR 700
Description: ELISA

Monkey Integrin alpha-X
E01A75239 96T
EUR 700
Description: ELISA

Porcine Integrin alpha-X
E01A57810 96T
EUR 700
Description: ELISA

Chicken Integrin alpha-X
E01A92677 96T
EUR 700
Description: ELISA

Integrin Alpha X (ITGAX) Antibody
20-abx113189
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  • 150 ul
  • 50 ul

Integrin Alpha X (ITGaX) Antibody
20-abx104960
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Integrin Alpha X (ITGaX) Antibody
20-abx129411
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Integrin Alpha X (ITGAX) Antibody
20-abx124171
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  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Integrin Alpha X (CD11c) Antibody
abx139631-01mg 0.1 mg
EUR 444

Integrin Alpha X (ITGAX) Antibody
abx033098-400ul 400 ul
EUR 627.6

Integrin Alpha X (ITGAX) Antibody
abx033098-80l 80 µl
EUR 343.2

Integrin Alpha X (CD11c) Antibody
abx200116-100ug 100 ug
EUR 477.6

 

Recombinant expression in human cells of lively integrin alpha 1 beta 1-blocking RTS-disintegrin jerdostatin.

  • Jerdostatin, an RTS quick disintegrin cloned from Protobothrops jerdonii and recombinantly produced in Escherichia coli, is a potent and particular antagonist of the alpha(1)beta(1) integrin. Jerdostatin selectively blocked the adhesion of alpha(1)beta(1)-Ok562 cell to collagens I and IV in vitro and angiogenesis in vivo.

 

  • Right here we report the recombinant manufacturing of jerdostatin in a mammalian cell system, a prerequisite for growing a conditional transgenic mouse to research the impact of systemic expression of jerdostatin on tumor growth. For correct export of jerdostatin, a secretion chief sequence was engineered on the protein’s N-terminus.

Mouse Integrin alpha-X ELISA equipment

  • A FLAG epitope was additionally included on the N-terminus of the mature disintegrin to facilitate its isolation and characterization of recombinant jerdostatin (rJerd).

 

  • This pRc-CMV/FLAG-rJerd assemble was transiently expressed in HEK-293 cells and was effectively secreted into the tradition medium. rJerd sure to recombinant soluble alpha(1)beta(1) integrin in a saturable and cation-independent method. Soluble rJerd additionally inhibited the binding of alpha(1)beta(1) integrin to the CB3 fragment of collagen IV in a dose-dependent method (IC(50) 570 nM). Mammalian cell-expressed jerdostatin disrupted the adhesion of RuGli cells to collagen IV.

 

  • Our outcomes spotlight pRc-CMV/FLAG-rJerd as an appropriate assemble for expressing soluble lively alpha(1)beta(1)-blocking jerdostatin in a mammalian cell system.

 

OBJECTIVE
To ascertain human integrin-alpha v adenovirus expressed in human liver most cancers SMMC-7721 cells and analyze the traits of integrin-alpha adhesion to liver most cancers cells.
METHODS
Human integrin-alpha v cDNA was cloned into the plasmid pAdTrack-CMV. Then, pAdTrack-integrin-alpha v was cotransformed with adenoviral spine vector into E. coli pressure BJ5183. The recombinant adenoviral plasmid was recognized by restriction evaluation with Pac I and transfected into HEK-293A cells to package deal and amplify recombinant adenovirus particles. Western blot confirmed that integrin-alpha v gene was precisely transcript and expressed in SMMC-

CFDA SE Cell Tracer Kit
K1023-1 1 kit
EUR 96
Description: The CFDA SE (carboxyfluorescein diacetate succinimidyl ester) Cell Tracer Kit is usually used in fluorescence analyses as a long-term tracer of cells. The Kit can be used for in vitro and in vivo labeling of cells to determine whether or not a cell is proliferating, especially.

Cell Strainer 100um Yellow Sterile Ind. Wrapped - PK50
ARG1222 PK50
EUR 253.8

Bolle Tracker PSJ Yellow Lens - EACH
SAF1912 EACH
EUR 23.36

Yellow 101
T35247-10mg 10mg Ask for price
Description: Yellow 101

Yellow 101
T35247-1g 1g Ask for price
Description: Yellow 101

Yellow 101
T35247-1mg 1mg Ask for price
Description: Yellow 101

Yellow 101
T35247-50mg 50mg Ask for price
Description: Yellow 101

Yellow 101
T35247-5mg 5mg Ask for price
Description: Yellow 101

Yellow 101
MBS5784735-INQUIRE INQUIRE Ask for price

Reactive Yellow 17 (Remazol Golden Yellow G)
67074 25 Gms
EUR 50.1
Description: Part B

FluoGreen Tracer, Stains Cell Nuclei and Cytoplasm
MBS555420-01mL 0.1mL
EUR 435

FluoGreen Tracer, Stains Cell Nuclei and Cytoplasm
MBS555420-5x01mL 5x0.1mL
EUR 1815

Methyl Yellow
12814-64 10G
EUR 32.2

MitoPB Yellow
D123550 25mg
EUR 15000

Yellow colour
PCT1315-250G 1 unit
EUR 60.87
Description: Yellow colour

Sunset yellow
GRM10357-25G 1 unit
EUR 10.08
Description: Sunset yellow

Methyl Yellow
M30640 25G
EUR 138.73

Alcian yellow
T29841-10mg 10mg Ask for price
Description: Alcian yellow

Alcian yellow
T29841-1g 1g Ask for price
Description: Alcian yellow

Alcian yellow
T29841-1mg 1mg Ask for price
Description: Alcian yellow

Alcian yellow
T29841-50mg 50mg Ask for price
Description: Alcian yellow

Alcian yellow
T29841-5mg 5mg Ask for price
Description: Alcian yellow

Alcian Yellow
MBS6120673-10g 10g
EUR 1200

Alcian Yellow
MBS6120673-25g 25g
EUR 1890

Alcian Yellow
MBS6120673-2g 2(g
EUR 550

Alcian Yellow
MBS6120673-50g 50g
EUR 2920

Alcian Yellow
MBS6120673-5g 5g
EUR 830

Alcian yellow
MBS5779473-5mg 5(mg
EUR 915

Alcian yellow
MBS5779473-5x5mg 5x5(mg
EUR 3970

Sunset yellow
MBS5760954-100mg 100mg
EUR 145

Sunset yellow
MBS5760954-5x100mg 5x100mg
EUR 500

Fluorol Yellow 088
F621725 1g
EUR 150
Description: 81-37-8

Pigment Yellow 180
P437800 10g
EUR 82
Description: 77804-81-0

mFluor™ Yellow 630 SE
1170 1 mg
EUR 60

Oxazole yellow
341177 1.0mg
EUR 390

Metanil Yellow
M321778 1g
EUR 76
Description: 587-98-4

Oxazole yellow
T33840-10mg 10mg Ask for price
Description: Oxazole yellow

Oxazole yellow
T33840-1g 1g Ask for price
Description: Oxazole yellow

Oxazole yellow
T33840-1mg 1mg Ask for price
Description: Oxazole yellow

Oxazole yellow
T33840-50mg 50mg Ask for price
Description: Oxazole yellow

Oxazole yellow
T33840-5mg 5mg Ask for price
Description: Oxazole yellow

Lucifer yellow
T32926-10mg 10mg Ask for price
Description: Lucifer yellow

Lucifer yellow
T32926-1g 1g Ask for price
Description: Lucifer yellow

Lucifer yellow
T32926-1mg 1mg Ask for price
Description: Lucifer yellow

Lucifer yellow
T32926-50mg 50mg Ask for price
Description: Lucifer yellow

Lucifer yellow
T32926-5mg 5mg Ask for price
Description: Lucifer yellow

Lucifer yellow
MBS5782491-5mg 5(mg
EUR 915

Lucifer yellow
MBS5782491-5x5mg 5x5(mg
EUR 3970

Oxazole yellow
MBS5783378-5mg 5(mg
EUR 915

Oxazole yellow
MBS5783378-5x5mg 5x5(mg
EUR 3970

Nuclear yellow
MBS3840913-100mg 100mg
EUR 405

Nuclear yellow
MBS3840913-200mg 200mg
EUR 650

Nuclear yellow
MBS3840913-50mg 50mg
EUR 260

Nuclear yellow
MBS3840913-5x200mg 5x200mg
EUR 2925

Dimethyl Yellow
D473790 10g
EUR 178
Description: 60-11-7

Gardenia yellow
T3S0175-10mg 10mg Ask for price
Description: Gardenia yellow

Gardenia yellow
T3S0175-1g 1g Ask for price
Description: Gardenia yellow

Gardenia yellow
T3S0175-1mg 1mg Ask for price
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Gardenia yellow
T3S0175-50mg 50mg Ask for price
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Gardenia yellow
T3S0175-5mg 5mg Ask for price
Description: Gardenia yellow

Acridine Yellow
T29619-10mg 10mg Ask for price
Description: Acridine Yellow

Acridine Yellow
T29619-1g 1g Ask for price
Description: Acridine Yellow

Acridine Yellow
T29619-1mg 1mg Ask for price
Description: Acridine Yellow

Acridine Yellow
T29619-50mg 50mg Ask for price
Description: Acridine Yellow

Acridine Yellow
T29619-5mg 5mg Ask for price
Description: Acridine Yellow

Acridine Yellow
MBS5779252-INQUIRE INQUIRE Ask for price

mFluor™ Yellow 630 acid
1145 5 mg
EUR 60

Quinoline yellow
RM877-25G 1 unit
EUR 27.08
Description: Quinoline yellow

Quinoline yellow
RM877-5G 1 unit
EUR 6.93
Description: Quinoline yellow

Safflower yellow
HY-N0938 50mg
EUR 159.6

Quinoline Yellow
Q701040 10g
EUR 86
Description: 8003-22-3

Safflower yellow
T19056-10mg 10mg Ask for price
Description: Safflower yellow

Safflower yellow
T19056-1g 1g Ask for price
Description: Safflower yellow

Safflower yellow
T19056-1mg 1mg Ask for price
Description: Safflower yellow

Safflower yellow
T19056-50mg 50mg Ask for price
Description: Safflower yellow

Safflower yellow
T19056-5mg 5mg Ask for price
Description: Safflower yellow

Safflower yellow
MBS5755887-50mg 50mg
EUR 150

Safflower yellow
MBS5755887-5mg 5mg
EUR 145

Safflower yellow
MBS5755887-5x50mg 5x50mg
EUR 515

Integrin Alpha X (CD11c) Antibody (PerCP)

7721 cells. Twenty 4 hours after transfection, the impact of integrin-alpha on adhesion of SMMC-7721 cells was detected by adhesion experiment.
RESULTS
Recombinant adenovirus vector of integrin-alpha v gene was efficiently constructed and excessive titers of recombinant adenovirus was obtained. Adhesion cell rely was considerably greater in integrin-alpha v transfected cells than in untransfected and Advert-null-transfected cells (P<0.01).
CONCLUSIONS
Over expression of integrin-alpha v promotes adhesion of SMMC-7721 cells, is a crucial molecular mechanism of tumor metastasis.

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