Measurement of the Interplay Between Recombinant I-domain from Integrin alpha 2 beta 1 and a Triple Helical Collagen Peptide with the GFOGER Binding Motif Utilizing Molecular Power Spectroscopy.
The position of the collagen-platelet interplay is of essential significance to the haemostatic response throughout each harm and pathogenesis of the blood vessel wall.
Of explicit curiosity is the excessive affinity interplay of the platelet transmembrane receptor, alpha 2 beta 1, accountable for agency attachment of platelets to collagen at and round harm websites.
We make use of single molecule power spectroscopy (SMFS) utilizing the atomic power microscope (AFM) to review the interplay of the I-domain from integrin alpha 2 beta 1 with an artificial collagen associated triple-helical peptide containing the high-affinity integrin-binding GFOGER motif, and a management peptide missing this sequence, known as GPP.
Human Integrin alpha- X, ITGAX ELISA KIT
By utilising artificial peptides on this method we’re in a position to research on the molecular stage subtleties that will in any other case be misplaced when contemplating cell-to-collagen matrix interactions utilizing ensemble strategies.
We display for the primary time the complexity of this interplay as illustrated by the advanced multi-peaked power spectra and verify specificity utilizing management blocking experiments. As well as we observe particular interplay of the GPP peptide sequence with the I-domain. We suggest a mannequin to clarify these observations.
Integrin Alpha X (CD11c) Antibody (PE)

Human Integrin alpha-X ELISA kit |
E01I0494-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A sandwich ELISA for quantitative measurement of Human Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Integrin alpha-X ELISA kit |
E01I0494-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Human Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Integrin alpha-X ELISA kit |
E01I0494-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Human Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Integrin Alpha X (ITGaX) Protein |
20-abx166474 |
Abbexa |
-
EUR 760.80
-
EUR 326.40
-
EUR 2281.20
-
EUR 895.20
-
EUR 543.60
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Mouse Integrin alpha-X (Itgax) |
1-CSB-BP874106MO1 |
Cusabio |
-
EUR 1008.00
-
EUR 398.40
-
EUR 2604.00
-
EUR 1362.00
-
EUR 1894.80
-
EUR 564.00
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Mouse Integrin alpha-X(Itgax),partial expressed in Baculovirus |
Integrin Alpha X (ITGaX) Antibody |
20-abx104960 |
Abbexa |
-
EUR 493.20
-
EUR 159.60
-
EUR 1378.80
-
EUR 678.00
-
EUR 376.80
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Integrin Alpha X (ITGAX) Antibody |
20-abx124171 |
Abbexa |
-
EUR 493.20
-
EUR 710.40
-
EUR 218.40
-
EUR 376.80
|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
Integrin Alpha X (CD11c) Antibody |
abx139631-01mg |
Abbexa |
0.1 mg |
EUR 444 |
|
Integrin Alpha X (ITGAX) Antibody |
20-abx113189 |
Abbexa |
|
|
|
Integrin Alpha X (ITGaX) Antibody |
20-abx129411 |
Abbexa |
-
EUR 510.00
-
EUR 159.60
-
EUR 1412.40
-
EUR 693.60
-
EUR 393.60
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Integrin Alpha X (ITGAX) Antibody |
abx033098-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Integrin Alpha X (ITGAX) Antibody |
abx033098-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Integrin Alpha X (CD11c) Antibody |
abx200116-100ug |
Abbexa |
100 ug |
EUR 477.6 |
|
Integrin Alpha X (CD11c) Antibody |
20-abx200679 |
Abbexa |
|
|
|
Integrin Alpha X (ITGAX) Antibody |
20-abx339188 |
Abbexa |
|
|
|
Integrin Alpha X (ITGAX) Antibody |
20-abx339189 |
Abbexa |
|
|
|
Integrin Alpha X (ITGAX) Antibody |
abx330439-100ul |
Abbexa |
100 ul |
EUR 510 |
|
Integrin Alpha X (CD11c) Antibody |
abx414593-01mg |
Abbexa |
0.1 mg |
EUR 910.8 |
|
Integrin Alpha X (CD11c) Antibody |
abx415362-01mg |
Abbexa |
0.1 mg |
EUR 543.6 |
|
Integrin Alpha X (CD11c) Antibody |
abx411892-50ug |
Abbexa |
50 ug |
EUR 811.2 |
|
Integrin Alpha X (CD11c) Antibody |
abx413004-025mg |
Abbexa |
0.25 mg |
EUR 678 |
|
Integrin Alpha X (CD11c) Antibody |
abx413008-01mg |
Abbexa |
0.1 mg |
EUR 526.8 |
|
Integrin Alpha X (CD11c) Antibody |
abx413011-25ug |
Abbexa |
25 ug |
EUR 326.4 |
|
Integrin Alpha X (CD11c) Antibody |
abx413356-2ml |
Abbexa |
2 ml |
EUR 727.2 |
|
Integrin Alpha X (CD11c) Antibody |
abx413688-02mg |
Abbexa |
0.2 mg |
EUR 678 |
|
Integrin Alpha X (CD11c) Antibody |
abx413691-01mg |
Abbexa |
0.1 mg |
EUR 526.8 |
|
Integrin Alpha X (CD11c) Antibody |
abx413694-25ug |
Abbexa |
25 ug |
EUR 326.4 |
|
Integrin Alpha X (ITGAX) Antibody |
20-abx322638 |
Abbexa |
|
|
|
Integrin Alpha X (CD11c) Antibody |
abx216334-100ug |
Abbexa |
100 ug |
EUR 526.8 |
|
Recombinant mouse Integrin alpha-X |
P2814 |
FN Test |
100ug |
Ask for price |
|
Description: Recombinant protein for mouse Integrin alpha-X |
Recombinant Integrin Alpha X (ITGaX) |
4-RPB159Hu01 |
Cloud-Clone |
-
EUR 539.33
-
EUR 267.60
-
EUR 1692.48
-
EUR 644.16
-
EUR 1168.32
-
EUR 436.80
-
EUR 4051.20
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Human Integrin Alpha X expressed in: E.coli |
Recombinant Integrin Alpha X (ITGaX) |
4-RPB159Mu01 |
Cloud-Clone |
-
EUR 560.83
-
EUR 273.60
-
EUR 1773.12
-
EUR 671.04
-
EUR 1222.08
-
EUR 451.20
-
EUR 4252.80
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Mouse Integrin Alpha X expressed in: E.coli |
Integrin Alpha X (ITGaX) Polyclonal Antibody (Human) |
4-PAB159Hu01 |
Cloud-Clone |
-
EUR 286.80
-
EUR 2869.20
-
EUR 717.60
-
EUR 358.80
-
EUR 253.20
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human Integrin Alpha X (ITGaX) |
Goat Integrin alpha-X ELISA kit |
E06I0494-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A sandwich ELISA for quantitative measurement of Goat Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Integrin alpha-X ELISA kit |
E06I0494-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Goat Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Integrin alpha-X ELISA kit |
E06I0494-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Goat Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Integrin alpha-X ELISA kit |
E08I0494-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A sandwich ELISA for quantitative measurement of Canine Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Integrin alpha-X ELISA kit |
E08I0494-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Canine Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Integrin alpha-X ELISA kit |
E08I0494-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Canine Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Integrin alpha-X ELISA kit |
E02I0494-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A sandwich ELISA for quantitative measurement of Rat Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Integrin alpha-X ELISA kit |
E02I0494-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Rat Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Integrin alpha-X ELISA kit |
E02I0494-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Rat Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Integrin alpha-X ELISA kit |
E04I0494-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A sandwich ELISA for quantitative measurement of Rabbit Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Integrin alpha-X ELISA kit |
E04I0494-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Rabbit Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Integrin alpha-X ELISA kit |
E04I0494-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Rabbit Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Integrin alpha-X ELISA kit |
E07I0494-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A sandwich ELISA for quantitative measurement of Porcine Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Integrin alpha-X ELISA kit |
E07I0494-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Porcine Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Integrin alpha-X ELISA kit |
E07I0494-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Porcine Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Integrin alpha-X ELISA kit |
E03I0494-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A sandwich ELISA for quantitative measurement of Mouse Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Integrin alpha-X ELISA kit |
E03I0494-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Mouse Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Integrin alpha-X ELISA kit |
E03I0494-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Mouse Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Integrin Alpha X (ITGaX) Protein |
20-abx167234 |
Abbexa |
-
EUR 777.60
-
EUR 326.40
-
EUR 2397.60
-
EUR 927.60
-
EUR 560.40
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Integrin Alpha X (CD11c) Antibody (PerCP) |
abx139632-100tests |
Abbexa |
100 tests |
EUR 577.2 |
|
Integrin Alpha X (CD11c) Antibody (PE) |
abx139633-100tests |
Abbexa |
100 tests |
EUR 577.2 |
|
Integrin Alpha X (CD11c) Antibody (APC) |
abx139634-100tests |
Abbexa |
100 tests |
EUR 577.2 |
|
Integrin Alpha X (CD11c) Antibody (FITC) |
abx139635-100tests |
Abbexa |
100 tests |
EUR 510 |
|
Integrin Alpha X (CD11c) Antibody (FITC) |
abx200117-100tests |
Abbexa |
100 tests |
EUR 477.6 |
|
Integrin Alpha X (CD11c) Antibody (PE) |
abx200118-100tests |
Abbexa |
100 tests |
EUR 794.4 |
|
Integrin Alpha X (CD11c) Antibody (APC) |
abx200119-100tests |
Abbexa |
100 tests |
EUR 1178.4 |
|
Integrin Alpha X (CD11c) Antibody (PerCP) |
abx200120-100tests |
Abbexa |
100 tests |
EUR 1528.8 |
|
Integrin Alpha X (CD11c) Antibody (Biotin) |
abx200121-100ug |
Abbexa |
100 ug |
EUR 460.8 |
|
Integrin Alpha X (CD11c) Antibody (APC) |
20-abx200672 |
Abbexa |
|
|
|
Integrin Alpha X (CD11c) Antibody (Biotin) |
20-abx200673 |
Abbexa |
|
|
|
Integrin Alpha X (CD11c) Antibody (FITC) |
20-abx200675 |
Abbexa |
-
EUR 343.20
-
EUR 276.00
-
EUR 376.80
|
|
|
Integrin Alpha X (CD11c) Antibody (PE) |
20-abx200676 |
Abbexa |
|
|
|
Integrin Alpha X (CD11c) Antibody (PerCP) |
20-abx200677 |
Abbexa |
|
|
|
CD11c/Integrin Alpha X (ITGAX) Antibody |
abx231412-100ug |
Abbexa |
100 ug |
EUR 577.2 |
|
CD11c/Integrin Alpha X (ITGAX) Antibody |
abx231413-100ug |
Abbexa |
100 ug |
EUR 610.8 |
|
Integrin Alpha X (CD11c) Antibody (FITC) |
abx413007-01mg |
Abbexa |
0.1 mg |
EUR 610.8 |
|
Integrin Alpha X (CD11c) Antibody (RPE) |
abx413009-100tests |
Abbexa |
100 tests |
EUR 710.4 |
|
Integrin Alpha X (CD11c) Antibody (RPE) |
abx413010-25tests |
Abbexa |
25 tests |
EUR 376.8 |
|
Integrin Alpha X (CD11c) Antibody (FITC) |
abx413689-01mg |
Abbexa |
0.1 mg |
EUR 610.8 |
|
Integrin Alpha X (CD11c) Antibody (FITC) |
abx413690-25ug |
Abbexa |
25 ug |
EUR 326.4 |
|
Integrin Alpha X (CD11c) Antibody (RPE) |
abx413692-100tests |
Abbexa |
100 tests |
EUR 710.4 |
|
Integrin Alpha X (CD11c) Antibody (RPE) |
abx413693-25tests025ml |
Abbexa |
25 tests (0.25 ml) |
EUR 376.8 |
|
Integrin Alpha X (CD11c) Antibody (Biotin) |
20-abx274308 |
Abbexa |
-
EUR 543.60
-
EUR 292.80
-
EUR 1545.60
-
EUR 744.00
-
EUR 410.40
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Monkey Integrin alpha-X ELISA kit |
E09I0494-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Integrin alpha-X ELISA kit |
E09I0494-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Integrin alpha-X ELISA kit |
E09I0494-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Integrin alpha X beta 2, CF |
PR15089CF |
Neuromics |
50 ug |
EUR 763.2 |
Human Integrin Alpha X / CD11C (ITGAX) ELISA Kit |
abx259467-96tests |
Abbexa |
96 tests |
EUR 1093.2 |
|
Integrin Alpha X (ITGaX) Polyclonal Antibody (Human), APC |
4-PAB159Hu01-APC |
Cloud-Clone |
-
EUR 399.60
-
EUR 3735.60
-
EUR 1046.40
-
EUR 507.60
-
EUR 258.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human Integrin Alpha X (ITGaX). This antibody is labeled with APC. |
Integrin Alpha X (ITGaX) Polyclonal Antibody (Human), Biotinylated |
4-PAB159Hu01-Biotin |
Cloud-Clone |
-
EUR 363.60
-
EUR 2809.20
-
EUR 836.40
-
EUR 442.80
-
EUR 259.20
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human Integrin Alpha X (ITGaX). This antibody is labeled with Biotin. |
Integrin Alpha X (ITGaX) Polyclonal Antibody (Human), Cy3 |
4-PAB159Hu01-Cy3 |
Cloud-Clone |
-
EUR 483.60
-
EUR 4930.80
-
EUR 1345.20
-
EUR 627.60
-
EUR 294.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human Integrin Alpha X (ITGaX). This antibody is labeled with Cy3. |
Integrin Alpha X (ITGaX) Polyclonal Antibody (Human), FITC |
4-PAB159Hu01-FITC |
Cloud-Clone |
-
EUR 344.40
-
EUR 3012.00
-
EUR 860.40
-
EUR 430.80
-
EUR 230.40
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human Integrin Alpha X (ITGaX). This antibody is labeled with FITC. |
Integrin Alpha X (ITGaX) Polyclonal Antibody (Human), HRP |
4-PAB159Hu01-HRP |
Cloud-Clone |
-
EUR 366.00
-
EUR 3256.80
-
EUR 926.40
-
EUR 459.60
-
EUR 243.60
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human Integrin Alpha X (ITGaX). This antibody is labeled with HRP. |
Integrin Alpha X (ITGaX) Polyclonal Antibody (Human), PE |
4-PAB159Hu01-PE |
Cloud-Clone |
-
EUR 344.40
-
EUR 3012.00
-
EUR 860.40
-
EUR 430.80
-
EUR 230.40
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human Integrin Alpha X (ITGaX). This antibody is labeled with PE. |
Recombinant expression in human cells of lively integrin alpha 1 beta 1-blocking RTS-disintegrin jerdostatin.
- Jerdostatin, an RTS quick disintegrin cloned from Protobothrops jerdonii and recombinantly produced in Escherichia coli, is a potent and particular antagonist of the alpha(1)beta(1) integrin. Jerdostatin selectively blocked the adhesion of alpha(1)beta(1)-Ok562 cell to collagens I and IV in vitro and angiogenesis in vivo.
- Right here we report the recombinant manufacturing of jerdostatin in a mammalian cell system, a prerequisite for growing a conditional transgenic mouse to research the impact of systemic expression of jerdostatin on tumor growth. For correct export of jerdostatin, a secretion chief sequence was engineered on the protein’s N-terminus.
Mouse Integrin alpha-X ELISA equipment
- A FLAG epitope was additionally included on the N-terminus of the mature disintegrin to facilitate its isolation and characterization of recombinant jerdostatin (rJerd).
- This pRc-CMV/FLAG-rJerd assemble was transiently expressed in HEK-293 cells and was effectively secreted into the tradition medium. rJerd sure to recombinant soluble alpha(1)beta(1) integrin in a saturable and cation-independent method. Soluble rJerd additionally inhibited the binding of alpha(1)beta(1) integrin to the CB3 fragment of collagen IV in a dose-dependent method (IC(50) 570 nM). Mammalian cell-expressed jerdostatin disrupted the adhesion of RuGli cells to collagen IV.
- Our outcomes spotlight pRc-CMV/FLAG-rJerd as an appropriate assemble for expressing soluble lively alpha(1)beta(1)-blocking jerdostatin in a mammalian cell system.
OBJECTIVE
To ascertain human integrin-alpha v adenovirus expressed in human liver most cancers SMMC-7721 cells and analyze the traits of integrin-alpha adhesion to liver most cancers cells.
METHODS
Human integrin-alpha v cDNA was cloned into the plasmid pAdTrack-CMV. Then, pAdTrack-integrin-alpha v was cotransformed with adenoviral spine vector into E. coli pressure BJ5183. The recombinant adenoviral plasmid was recognized by restriction evaluation with Pac I and transfected into HEK-293A cells to package deal and amplify recombinant adenovirus particles. Western blot confirmed that integrin-alpha v gene was precisely transcript and expressed in SMMC-
PTPN11 (Ab-580) Antibody |
CSB-PA961746-100ul |
Cusabio |
100ul |
EUR 379.2 |
|
Description: A polyclonal antibody against PTPN11 (Ab-580). Recognizes PTPN11 (Ab-580) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 |
MemBriteâ„¢ Fix 568/580 |
30095 |
Biotium |
1KIT |
EUR 772.8 |
Description: Minimum order quantity: 1 unit of 1KIT |
hsa-miR-580 Primers |
MPH01878 |
ABM |
150 ul / 10 uM |
EUR 145.2 |
PTPN11 (Ab-580) Antibody |
CSB-PA961746- |
Cusabio |
each |
EUR 402 |
|
Description: A polyclonal antibody against PTPN11 (Ab-580). Recognizes PTPN11 (Ab-580) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 |
SHP-2 (Ab-580) Antibody |
21320-100ul |
SAB |
100ul |
EUR 302.4 |
SHP-2 (Ab-580) Antibody |
21320-50ul |
SAB |
50ul |
EUR 224.4 |
PYK2 (Phospho-Tyr579/580) Antibody |
13230-100ul |
SAB |
100ul |
EUR 302.4 |
PYK2 (Phospho-Tyr579/580) Antibody |
13230-50ul |
SAB |
50ul |
EUR 224.4 |
anti-SHP-2 (Ab-580) |
LF-PA20454 |
Abfrontier |
100 ul |
EUR 400.8 |
Description: Rabbit polyclonal to SHP-2 |
hsa-miR-580 miRNA Inhibitor |
MIH03164 |
ABM |
2 x 5.0 nmol |
EUR 211.2 |
hsa-miR-580-3p Primers |
MPH03331 |
ABM |
150 ul / 10 uM |
EUR 145.2 |
hsa-miR-580-5p Primers |
MPH03332 |
ABM |
150 ul / 10 uM |
EUR 145.2 |
hsa-miR-580 miRNA Antagomir |
MNH03164 |
ABM |
2 x 5.0 nmol |
EUR 394.8 |
Serpin A1c Recombinant Protein |
91-580 |
ProSci |
0.05 mg |
EUR 821.4 |
Description: Alpha-1-antitrypsin 1-3(SERPIN A1) is a secreted protein and belongs to the serpin family. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. Mouse SERPIN A1 is a serine protease inhibitor whose targets include elastase,plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema orliver disease. Several transcript variants encoding the same protein have been found for this gene. |
Kallikrein 7 Recombinant Protein |
92-580 |
ProSci |
0.05 mg |
EUR 821.4 |
Description: Kallikrein7, also named as stratum corneum chymotryptic enzyme (SCCE), is a secreted protein of the Kallikrein-related peptidase (KLK) family. This family contains fifteen homologous secreted serine endopeptidases and plays a significant role in various physiological processes, including skin desquamation, semen liquefaction, neural plasticity, and body fluid homeostasis. In skin KLK5, KLK 7 and KLK14 are able to degrade corneodesmosomes, which leads to desquamation of skin surface cells. KLK activation is believed to be mediated through highly organized proteolytic cascades, regulated through a series of feedback loops, inhibitors, auto-degradation and internal cleavages. Studies have shown that one potential physiological activator for KLK7 is KLK5. Along with KLK14, these three kallikreins form a proteolytic cascade in the stratum corneum. KLK7 is primarily expressed in the skin but is also detected at relatively high levels in esophagus, heart, liver, central nervous system, kidney, pancreas, mammary and salivary glands. |
Sunset Yellow (BSA) |
20-abx165822 |
Abbexa |
-
EUR 693.60
-
EUR 309.60
-
EUR 2064.00
-
EUR 828.00
-
EUR 510.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Sunset Yellow (OVA) |
20-abx165823 |
Abbexa |
-
EUR 693.60
-
EUR 309.60
-
EUR 2064.00
-
EUR 828.00
-
EUR 510.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Alizarin yellow GG |
AB0011 |
Bio Basic |
25g |
EUR 70.44 |
|
Alizarin yellow R |
AB0012 |
Bio Basic |
25g |
EUR 70.44 |
|
C.I.Vat Yellow 2 |
20-abx181248 |
Abbexa |
|
|
|
Pigment Yellow 42 |
abx185544-100g |
Abbexa |
100 g |
EUR 427.2 |
|
lucifer yellow cadaverine |
80018 |
Biotium |
25MG |
EUR 178.8 |
Description: Minimum order quantity: 1 unit of 25MG |
0.6ML MICROTUBES. YELLOW |
MCT-060-Y |
CORNING |
1000/pk |
EUR 219.6 |
Description: Micro Tubes; Micro Tubes Push Cap- Axygen |
1.7ML MICROTUBES. YELLOW |
MCT-175-Y |
CORNING |
500/pk |
EUR 139.2 |
Description: Micro Tubes; Micro Tubes Push Cap- Axygen |
Hydroxysafflor yellow A |
N1526-20 |
ApexBio |
20 mg |
EUR 338.4 |
Description: Extracted from Carthamus tinctorius L;Store the product in sealed,cool and dry condition |
Anhydrosafflor yellow B |
TBW01543 |
ChemNorm |
5mg |
Ask for price |
200UL YELLOW TIPS |
T-200-Y |
CORNING |
1000/pk |
EUR 264 |
Description: Non Filter Tips; Pipette Tips - Axygen |
Safflor Yellow A |
TBZ2652 |
ChemNorm |
unit |
Ask for price |
Labeling Tape, Yellow |
TP004Y |
Bio Basic |
1ROLLS, 1 UNIT |
EUR 64.42 |
|
Yellow Calibration Particles |
YCP-70-5 |
Spherotech |
5 mL |
EUR 388.8 |
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
Yellow Fluorescent Protein |
4998-100 |
Biovision |
each |
EUR 470.4 |
Yellow Fluorescent Protein |
4998-1000 |
Biovision |
each |
EUR 3015.6 |
Yellow Fluorescent Protein |
4998-5000 |
Biovision |
each |
EUR 8215.2 |
PYK2 (Phospho-Tyr579/580) Conjugated Antibody |
C13230 |
SAB |
100ul |
EUR 476.4 |
Phospho-PYK2 (Tyr579/580) Blocking Peptide |
AF7383-BP |
Affbiotech |
1mg |
EUR 234 |
Mesothelin / MSLN (296-580) Recombinant Protein |
11-216 |
ProSci |
0.1 mg |
EUR 714.3 |
Description: Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro. |
MemBriteâ„¢ Fix 568/580, Trial Size |
30095-T |
Biotium |
1KIT |
EUR 266.4 |
Description: Minimum order quantity: 1 unit of 1KIT |
Zinc Finger Protein 580 (ZNF580) Antibody |
abx030431-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Zinc Finger Protein 580 (ZNF580) Antibody |
abx030431-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Recombinant human Zinc finger protein 580 |
P2142 |
FN Test |
100ug |
Ask for price |
|
Description: Recombinant protein for human Zinc finger protein 580 |
hsa-miR-580-3p miRNA Inhibitor |
MIH03165 |
ABM |
2 x 5.0 nmol |
EUR 211.2 |
hsa-miR-580-5p miRNA Inhibitor |
MIH03166 |
ABM |
2 x 5.0 nmol |
EUR 211.2 |
hsa-miR-580-3p miRNA Antagomir |
MNH03165 |
ABM |
2 x 5.0 nmol |
EUR 394.8 |
hsa-miR-580-5p miRNA Antagomir |
MNH03166 |
ABM |
2 x 5.0 nmol |
EUR 394.8 |
Long trebler phosphoramidite |
A8151-250 |
ApexBio |
250 mg |
EUR 408 |
Description: Long trebler phosphoramidite is a branching reagent for oligonucleotide synthesis allowing to synthesize branched DNA structures using standard DNA synthesizer. |
CytoFix™ BCECF, AM *Optimized for long term cellular pH tracking* |
21200 |
AAT Bioquest |
1 mg |
EUR 318 |
Sunset Yellow (SY) Antibody |
20-abx100868 |
Abbexa |
-
EUR 510.00
-
EUR 159.60
-
EUR 1412.40
-
EUR 693.60
-
EUR 393.60
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Integrin Alpha X (CD11c) Antibody (PerCP)
7721 cells. Twenty 4 hours after transfection, the impact of integrin-alpha on adhesion of SMMC-7721 cells was detected by adhesion experiment.
RESULTS
Recombinant adenovirus vector of integrin-alpha v gene was efficiently constructed and excessive titers of recombinant adenovirus was obtained. Adhesion cell rely was considerably greater in integrin-alpha v transfected cells than in untransfected and Advert-null-transfected cells (P<0.01).
CONCLUSIONS
Over expression of integrin-alpha v promotes adhesion of SMMC-7721 cells, is a crucial molecular mechanism of tumor metastasis.