Immunization in opposition to leukemia inhibitory issue and its receptor suppresses tumor formation of breast most cancers initiating cells in BALB/c mouse
- Immunotherapy is a promising method for particular focusing on of most cancers cells. Leukemia inhibitory issue (LIF) regulates a number of options of cancers and most cancers stem cells (CSCs) by binding to LIF receptor (LIFR). On this research, we investigated the consensus of LIF and LIFR immunization on the expansion of mouse mammary tumors. For this objective, mouse LIF and LIFR had been designed as truncated proteins, expressed in E. coli after which injected to mice as particular person and blended antigens.
- The outcomes confirmed the manufacturing of neutralizing antibodies and secretion of interferon-γ and interleukin-2 in response to immunization. In proceed, the immunized mice had been subjected for tumor formation problem by inoculation of the breast CSCs derived from MC4-L2 cells.
- Improvement of the breast tumors was noticed in all of the management mice, whereas the tumors appeared in 75% of animals within the LIF group. LIFR injection, individually or together with LIF, strongly inhibited the tumor development to solely 25% of the mice. Furthermore, a delay in tumor look was noticed within the immunized mice in comparison with the controls.
- Immunostaining of the tumor sections confirmed the expression of LIF and LIFR. In conclusion, LIF and LIFR is likely to be efficient targets for immunotherapy of the tumors that specific these proteins.
- Earlier research have proven that some inflammatory cytokines promote the expression of corticotropin-releasing hormone (CRH) in trophoblasts throughout being pregnant and that placental CRH might induce the manufacturing of adrenocorticotropic hormone (ACTH) in people.
- Nevertheless, whether or not the identical is true in rodent placenta stays unclear. On this research, we examined the impact of pro-inflammatory cytokine LIF on the induction of CRH in mouse trophoblast stem cells (mTSCs). Throughout differentiation, the CRH ranges in mTSCs progressively elevated.
Mouse Leukemia Inhibitory Issue (LIF) ELISA Package
- On days three and 5 after LIF supplementation, Crh expression within the differentiated mTSCs was considerably elevated with LIF remedy than these with out LIF remedy. Furthermore, the CRH focus within the tradition media elevated. Thereafter, we examined the contribution of the downstream pathways of LIF to CRH induction in differentiated mTSCs.
- The LIF-induced upregulation of CRH was attenuated by inhibition of PI3K/AKT and MAPK phosphorylation however not by inhibition of JAK/STAT3. Due to this fact, in mTSCs, LIF elevated Crh expression by activation of the PI3K/AKT and MAPK pathways however not by the JAK/STAT3 pathway. The current research means that mTSC is a perfect in vitro mannequin for finding out regulation and performance of placental CRH.
Bovine Leukemia Inhibitory Issue (LIF) ELISA Package
Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 613.2 |
Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 850.8 |
Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 586.8 |
Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 812.4 |
anti-LIF |
YF-PA12952 |
Abfrontier |
50 ug |
EUR 435.6 |
Description: Mouse polyclonal to LIF |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-p-48T |
DL Develop |
48T |
EUR 656.4 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-p-96T |
DL Develop |
96T |
EUR 858 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-Ra-48T |
DL Develop |
48T |
EUR 609.6 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-Ra-96T |
DL Develop |
96T |
EUR 793.2 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-b-48T |
DL Develop |
48T |
EUR 656.4 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-b-96T |
DL Develop |
96T |
EUR 858 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-c-48T |
DL Develop |
48T |
EUR 632.4 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-c-96T |
DL Develop |
96T |
EUR 825.6 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-Hu-48T |
DL Develop |
48T |
EUR 456 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-Hu-96T |
DL Develop |
96T |
EUR 582 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-b-48Tests |
Reddot Biotech |
48 Tests |
EUR 696 |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-b-96Tests |
Reddot Biotech |
96 Tests |
EUR 968.4 |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-c-48Tests |
Reddot Biotech |
48 Tests |
EUR 668.4 |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-c-96Tests |
Reddot Biotech |
96 Tests |
EUR 928.8 |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 459.6 |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 630 |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-p-48Tests |
Reddot Biotech |
48 Tests |
EUR 696 |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-p-96Tests |
Reddot Biotech |
96 Tests |
EUR 968.4 |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 640.8 |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 890.4 |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-b-48Tests |
Reddot Biotech |
48 Tests |
EUR 666 |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-b-96Tests |
Reddot Biotech |
96 Tests |
EUR 925.2 |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-c-48Tests |
Reddot Biotech |
48 Tests |
EUR 639.6 |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-c-96Tests |
Reddot Biotech |
96 Tests |
EUR 888 |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 440.4 |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 602.4 |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-p-48Tests |
Reddot Biotech |
48 Tests |
EUR 666 |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-p-96Tests |
Reddot Biotech |
96 Tests |
EUR 925.2 |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 613.2 |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 850.8 |
Rabbit Polyclonal antibody Anti-CRBN |
Anti-CRBN |
ImmunoStep |
50 µg |
EUR 418.8 |
Anti-LIF Antibody |
A01400 |
BosterBio |
100ug/vial |
EUR 352.8 |
anti- LIF antibody |
FNab09804 |
FN Test |
100µg |
EUR 658.5 |
|
Description: Antibody raised against LIF |
Anti-LIF Antibody |
PA1562 |
BosterBio |
100ug/vial |
EUR 352.8 |
Anti-LIF Antibody |
PB9036 |
BosterBio |
100ug/vial |
EUR 400.8 |
Anti-LIF antibody |
STJ96472 |
St John's Laboratory |
200 µl |
EUR 236.4 |
Description: Rabbit polyclonal to LIF. |
Anti-LIF antibody |
STJ29846 |
St John's Laboratory |
100 µl |
EUR 332.4 |
Description: The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
Mouse LIF shRNA Plasmid |
20-abx971321 |
Abbexa |
|
|
|
Mouse LIF ELISA Kit |
EML0014 |
Abclonal |
96Tests |
EUR 625.2 |
Recombinant Mouse LIF Protein |
R01400-1 |
BosterBio |
100ug/vial |
EUR 352.8 |
Polyclonal Goat anti-GST α-form |
GST-ANTI-1 |
Detroit R&D |
50 uL |
EUR 336 |
Polyclonal Goat anti-GST μ-form |
GST-ANTI-2 |
Detroit R&D |
50 uL |
EUR 336 |
Polyclonal Goat anti-GST p-form |
GST-ANTI-3 |
Detroit R&D |
50 uL |
EUR 336 |
LIF Antibody |
6245-002mg |
ProSci |
0.02 mg |
EUR 206.18 |
|
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation. |
LIF Antibody |
6245-01mg |
ProSci |
0.1 mg |
EUR 523.7 |
|
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation. |
LIF Peptide |
6245P |
ProSci |
0.05 mg |
EUR 197.7 |
Description: (IN) LIF peptide |
LIF antibody |
70R-12299 |
Fitzgerald |
100 ug |
EUR 483.6 |
Description: Rabbit polyclonal LIF antibody |
LIF Peptide |
43-200P |
ProSci |
0.1 mg |
EUR 405.6 |
LIF antibody |
70R-14100 |
Fitzgerald |
100 ug |
EUR 386.4 |
Description: Affinity purified Rabbit polyclonal LIF antibody |
LIF antibody |
70R-51295 |
Fitzgerald |
100 ul |
EUR 292.8 |
Description: Purified Polyclonal LIF antibody |
LIF protein |
30R-2771 |
Fitzgerald |
10 ug |
EUR 442.8 |
Description: Purified recombinant Human LIF protein |
LIF protein |
30R-2772 |
Fitzgerald |
10 ug |
EUR 586.8 |
Description: Purified recombinant Mouse LIF protein |
LIF Antibody |
36950-100ul |
SAB |
100ul |
EUR 302.4 |
LIF Antibody |
25283-100ul |
SAB |
100ul |
EUR 468 |
LIF antibody |
70R-6230 |
Fitzgerald |
50 ug |
EUR 560.4 |
Description: Rabbit polyclonal LIF antibody |
LIF Antibody |
1-CSB-PA198481 |
Cusabio |
|
|
|
Description: A polyclonal antibody against LIF. Recognizes LIF from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:10000, IHC:1:25-1:100 |
LIF Antibody |
1-CSB-PA004761 |
Cusabio |
|
|
|
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000 |
LIF Antibody |
1-CSB-PA012928ESR2HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: ELISA |
LIF, human |
MO16101 |
Neuromics |
100 ug |
EUR 825.6 |
Human CellExp? LIF, mouse recombinant |
7268-10 |
Biovision |
|
EUR 444 |
LIF ELISA Kit (Mouse) (OKBB00760) |
OKBB00760 |
Aviva Systems Biology |
96 Wells |
EUR 606 |
Description: Description of target: Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The LIF was mapped gene to 22q11-q12.2 by Southern analysis of a series of mouse/human somatic cell hybrids and by in situ hybridization to the chromosomes of 2 normal males and some individuals with chromosomal rearrangements. The gene maps between the Philadelphia translocation BCR1 and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: <10pg/ml |
Mouse LIF PicoKine ELISA Kit |
EK0580 |
BosterBio |
96 wells |
EUR 510 |
Description: For quantitative detection of mouse LIF in cell culture supernates, serum and plasma (heparin, EDTA). |
LIF ELISA Kit (Mouse) (OKEH04045) |
OKEH04045 |
Aviva Systems Biology |
96 Wells |
EUR 652.8 |
Description: Description of target: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.039 ng/mL |
Lif ORF Vector (Mouse) (pORF) |
ORF049145 |
ABM |
1.0 ug DNA |
EUR 607.2 |
Canine Leukemia Inhibitory Issue (LIF) ELISA Package

Manufacturing of soluble bioactive mouse leukemia inhibitory issue from Escherichia coli utilizing MBP tag.
Embryonic stem cells and induced pluripotent stem cells rely on one in every of cytokines referred to as leukemia inhibitory issue (LIF) to retain their undifferentiated state and pluripotency.
Integrin alpha 5 Antibody |
AF5200 |
Affbiotech |
200ul |
EUR 420 |
Integrin alpha 5 Antibody |
49269-100ul |
SAB |
100ul |
EUR 399.6 |
Integrin alpha 5 Antibody |
49269-50ul |
SAB |
50ul |
EUR 286.8 |
Integrin alpha-5 Antibody |
abx234347-100ug |
Abbexa |
100 ug |
EUR 610.8 |
|
Anti-Integrin alpha 5 Rabbit Monoclonal Antibody |
M01911 |
BosterBio |
100ug/vial |
EUR 476.4 |
Description: Rabbit Monoclonal Integrin alpha 5 Antibody. Validated in IP, IF, WB and tested in Human, Mouse, Rat. |
Alpha V Beta 5 Integrin, Peptide Aptamer, FITC labelled |
AP-301-F |
Alpha Diagnostics |
1 mg |
Ask for price |
Human Integrin Alpha 5 (ITGa5) Protein |
20-abx067272 |
Abbexa |
-
EUR 794.40
-
EUR 326.40
-
EUR 2448.00
-
EUR 944.40
-
EUR 577.20
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Integrin alpha 5 Conjugated Antibody |
C49269 |
SAB |
100ul |
EUR 476.4 |
Integrin alpha-5 Recombinant Protein |
91-433 |
ProSci |
0.05 mg |
EUR 651.3 |
Description: Integrin alpha-5 belongs to the Integrin alpha chain family and contains 7 FG-GAP repeats. Integrin alpha-5 joins with Integrin- beta 1 to form a fibronectin and laminin receptor which recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. It is expressed on fibroblasts, endothelial cells, peripheral T cells and platelets. Integrin alpha-5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains. In addition to adhesion, ITGA5 participates in cell-surface mediated signalling. |
Integrin Alpha 5 (ITGa5) Antibody |
20-abx101634 |
Abbexa |
-
EUR 493.20
-
EUR 159.60
-
EUR 1378.80
-
EUR 678.00
-
EUR 376.80
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Integrin Alpha 5 (ITGa5) Antibody |
20-abx101635 |
Abbexa |
-
EUR 560.40
-
EUR 159.60
-
EUR 1612.80
-
EUR 760.80
-
EUR 427.20
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Integrin Alpha 5 (ITGa5) Antibody |
20-abx172956 |
Abbexa |
|
|
|
Integrin Alpha 5 (ITGA5) Antibody |
20-abx113185 |
Abbexa |
|
|
|
Integrin Alpha 5 (ITGa5) Antibody |
20-abx129339 |
Abbexa |
-
EUR 510.00
-
EUR 159.60
-
EUR 1412.40
-
EUR 693.60
-
EUR 393.60
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Integrin Alpha 5 (ITGA5) Antibody |
20-abx006811 |
Abbexa |
-
EUR 493.20
-
EUR 710.40
-
EUR 218.40
-
EUR 376.80
|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
Integrin Alpha 5 (ITGA5) Antibody |
20-abx000873 |
Abbexa |
|
|
|
Integrin Alpha 5 (ITGA5) Antibody |
abx011678-100ul |
Abbexa |
100 ul |
EUR 493.2 |
|
Integrin Alpha 5 (ITGA5) Antibody |
abx026655-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Integrin Alpha 5 (ITGA5) Antibody |
abx026655-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Integrin alpha 5 Rabbit mAb |
A19069-100ul |
Abclonal |
100 ul |
EUR 492 |
Integrin alpha 5 Rabbit mAb |
A19069-200ul |
Abclonal |
200 ul |
EUR 685.2 |
Integrin alpha 5 Rabbit mAb |
A19069-20ul |
Abclonal |
20 ul |
EUR 265.2 |
Integrin alpha 5 Rabbit mAb |
A19069-50ul |
Abclonal |
50 ul |
EUR 344.4 |
Integrin Alpha 5 (ITGA5) Antibody |
20-abx338742 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Integrin Alpha 5 (ITGA5) Antibody |
20-abx323564 |
Abbexa |
|
|
|
Integrin Alpha 5 (ITGA5) Antibody |
20-abx325454 |
Abbexa |
|
|
|
Integrin Alpha 5 (ITGA5) Antibody |
20-abx327245 |
Abbexa |
|
|
|
Integrin Alpha 5 (ITGa5) Antibody |
20-abx179024 |
Abbexa |
|
|
|
Integrin alpha 5 (CD49e) Antibody |
abx216264-100ug |
Abbexa |
100 ug |
EUR 526.8 |
|
Recombinant Integrin Alpha 5 (ITGa5) |
4-RPB287Bo01 |
Cloud-Clone |
-
EUR 668.35
-
EUR 302.40
-
EUR 2176.32
-
EUR 805.44
-
EUR 1490.88
-
EUR 523.20
-
EUR 5260.80
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Bovine Integrin Alpha 5 expressed in: E.coli |
Recombinant Integrin Alpha 5 (ITGa5) |
4-RPB287Hu01 |
Cloud-Clone |
-
EUR 571.58
-
EUR 276.00
-
EUR 1813.44
-
EUR 684.48
-
EUR 1248.96
-
EUR 458.40
-
EUR 4353.60
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Human Integrin Alpha 5 expressed in: E.coli |
Recombinant Integrin Alpha 5 (ITGa5) |
4-RPB287Mu01 |
Cloud-Clone |
-
EUR 571.58
-
EUR 276.00
-
EUR 1813.44
-
EUR 684.48
-
EUR 1248.96
-
EUR 458.40
-
EUR 4353.60
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Mouse Integrin Alpha 5 expressed in: E.coli |
Integrin alpha 5 Antibody (ITGA5) |
F49220-0.08ML |
NSJ Bioreagents |
0.08 ml |
EUR 165 |
Integrin alpha 5 Antibody (ITGA5) |
F41992-0.08ML |
NSJ Bioreagents |
0.08 ml |
EUR 165 |
anti-Integrin beta 5 |
YF-PA24023 |
Abfrontier |
50 ul |
EUR 400.8 |
Description: Mouse polyclonal to Integrin beta 5 |
anti-Integrin beta 5 |
YF-PA12792 |
Abfrontier |
100 ug |
EUR 483.6 |
Description: Rabbit polyclonal to Integrin beta 5 |
Rabbit Anti-Human Alpha Defensin 5 (NP-5),antiserum #1 |
HDEFA51-S |
Alpha Diagnostics |
100 ul |
EUR 548.4 |
Human Integrin alpha-5(ITGA5) Elisa kit |
CSB-E17350h-24T |
Cusabio |
1 plate of 24 wells |
EUR 198 |
|
Description: Quantitativesandwich ELISA kit for measuring Human Integrin alpha-5 (ITGA5) in samples from serum, plasma, cell culture supernates, urine, cerebrospinalfluid (CSF). A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Human Integrin alpha-5(ITGA5) Elisa kit |
1-CSB-E17350h |
Cusabio |
-
EUR 1080.00
-
EUR 6571.20
-
EUR 3480.00
|
- 1 plate of 96 wells
- 10 plates of 96 wells each
- 5 plates of 96 wells each
|
|
Description: Quantitativesandwich ELISA kit for measuring Human Integrin alpha-5(ITGA5) in samples from serum, plasma, cell culture supernates, urine, cerebrospinalfluid(CSF). Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
Human ITGA5/ Integrin alpha-5 ELISA Kit |
E1342Hu |
Sunlong |
1 Kit |
EUR 726 |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
DLR-ITGa5-Hu-48T |
DL Develop |
48T |
EUR 597.6 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Integrin Alpha 5 (ITGa5) in samples from tissue homogenates, cell lysates or other biological fluids. |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
DLR-ITGa5-Hu-96T |
DL Develop |
96T |
EUR 776.4 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Integrin Alpha 5 (ITGa5) in samples from tissue homogenates, cell lysates or other biological fluids. |
ELISA kit for Human Integrin alpha-5 |
EK4913 |
SAB |
96 tests |
EUR 804 |
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Integrin alpha-5 in samples from serum, plasma, tissue homogenates and other biological fluids. |
Human ITGA5(Integrin alpha-5) ELISA Kit |
EH2445 |
FN Test |
96T |
EUR 681.12 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
20-abx151987 |
Abbexa |
-
EUR 8534.40
-
EUR 4550.40
-
EUR 1054.80
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Human Integrin Alpha 5 (ITGA5) ELISA Kit |
abx571172-96tests |
Abbexa |
96 tests |
EUR 886.8 |
|
Human Integrin Alpha 5 (ITGa5) CLIA Kit |
20-abx492595 |
Abbexa |
-
EUR 9567.60
-
EUR 5095.20
-
EUR 1177.20
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Integrin Alpha 5 (ITGa5) Polyclonal Antibody (Human) |
4-PAB287Hu01 |
Cloud-Clone |
-
EUR 286.80
-
EUR 2869.20
-
EUR 717.60
-
EUR 358.80
-
EUR 253.20
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
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Description: A Rabbit polyclonal antibody against Human Integrin Alpha 5 (ITGa5) |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
RD-ITGa5-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 600 |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
RD-ITGa5-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 830.4 |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
RDR-ITGa5-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 626.4 |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
RDR-ITGa5-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 868.8 |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
SEB287Hu-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 5402.92 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids. |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
SEB287Hu-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 550.13 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids. |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
SEB287Hu-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 734.46 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids. |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
4-SEB287Hu |
Cloud-Clone |
-
EUR 5463.60
-
EUR 2886.00
-
EUR 735.60
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Integrin Alpha 5 (ITGa5) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. This is a cost efficient bulk pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits to avoid unsealing the plates and reagents that won't be immediately used. |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
SEB287Hu-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 2945.08 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids. |
Mouse Anti Human Integrin Alpha V Beta 5 Monoclonal Antibody |
CABT-49626MH |
Creative Diagnostics |
0.1 mg |
EUR 1650 |
Anti-alpha 5 beta 1 Integrin (Volociximab), Human IgG4 Antibody |
A1092-200 |
Biovision |
|
EUR 874.8 |
Anti-Integrin alpha antibody |
STJ96613 |
St John's Laboratory |
200 µl |
EUR 236.4 |
Description: Rabbit polyclonal to Integrin alpha4. |
Anti-Integrin alpha antibody |
STJ96825 |
St John's Laboratory |
200 µl |
EUR 236.4 |
Description: Rabbit polyclonal to Integrin alphaD. |
Anti-Integrin alpha antibody |
STJ97273 |
St John's Laboratory |
200 µl |
EUR 236.4 |
Description: Rabbit polyclonal to Integrin alphaL. |
Anti-Integrin alpha antibody |
STJ97283 |
St John's Laboratory |
200 µl |
EUR 236.4 |
Description: Rabbit polyclonal to Integrin alpha2. |
Anti-Integrin alpha antibody |
STJ97284 |
St John's Laboratory |
200 µl |
EUR 236.4 |
Description: Integrin alpha5 is a protein encoded by the ITGA5 gene which is approximately 114,5 kDa. Integrin alpha5 is localised to the cell membrane and is involved in the MAPK-Erk pathway, apoptotic pathways, integrin pathway, focal adhesion and blood-brain barrier and immune cell transmigration. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signalling. Integrin alpha5 is expressed in the liver, lung, nervous system, bone marrow and blood. Mutations in the ITGA5 gene may result in colon carcinoma and congenital epulis STJ97284 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Integrin alpha5 protein. |
Anti-Integrin alpha antibody |
STJ97285 |
St John's Laboratory |
200 µl |
EUR 236.4 |
Description: Rabbit polyclonal to Integrin alpha6. |
Anti-Integrin alpha antibody |
STJ97657 |
St John's Laboratory |
200 µl |
EUR 236.4 |
Description: Rabbit polyclonal to Integrin alpha3. |
Anti-Integrin alpha antibody |
STJ93727 |
St John's Laboratory |
200 µl |
EUR 236.4 |
Description: Rabbit polyclonal to Integrin alpha3. |
Anti-Integrin alpha antibody |
STJ93728 |
St John's Laboratory |
200 µl |
EUR 236.4 |
Description: Rabbit polyclonal to Integrin alpha4. |
Anti-Integrin alpha antibody |
STJ93729 |
St John's Laboratory |
200 µl |
EUR 236.4 |
Description: Rabbit polyclonal to Integrin alphaE. |
Anti-Integrin alpha antibody |
STJ93730 |
St John's Laboratory |
200 µl |
EUR 236.4 |
Description: Rabbit polyclonal to Integrin alphaV. |
Anti-Integrin alpha antibody |
STJ98181 |
St John's Laboratory |
100 µl |
EUR 280.8 |
Description: Mouse monoclonal to Integrin alpha5. |
Anti-Integrin alpha antibody |
STJ98510 |
St John's Laboratory |
100 µl |
EUR 280.8 |
Description: Mouse monoclonal to Integrin alpha5. |
Anti-Integrin alpha antibody |
STJ98511 |
St John's Laboratory |
100 µl |
EUR 280.8 |
Description: Mouse monoclonal to Integrin alpha6. |
Anti-Integrin alpha antibody |
STJ98709 |
St John's Laboratory |
200 µl |
EUR 236.4 |
Description: Rabbit polyclonal to Integrin alpha1. |
Anti-Integrin alpha antibody |
STJ98737 |
St John's Laboratory |
200 µl |
EUR 236.4 |
Description: Rabbit polyclonal to Integrin alphaX. |
Anti-Integrin alpha antibody |
STJ98984 |
St John's Laboratory |
200 µl |
EUR 236.4 |
Description: Rabbit polyclonal to Integrin alphaE. |
anti-Integrin alpha 6 |
YF-PA24004 |
Abfrontier |
50 ul |
EUR 400.8 |
Description: Mouse polyclonal to Integrin alpha 6 |
anti-Integrin alpha 1 |
YF-PA24011 |
Abfrontier |
50 ul |
EUR 400.8 |
Description: Mouse polyclonal to Integrin alpha 1 |
anti-Integrin alpha 4 |
YF-PA24013 |
Abfrontier |
50 ul |
EUR 400.8 |
Description: Mouse polyclonal to Integrin alpha 4 |
anti-Integrin alpha 9 |
YF-PA24015 |
Abfrontier |
50 ul |
EUR 400.8 |
Description: Mouse polyclonal to Integrin alpha 9 |
anti-Integrin alpha E |
YF-PA24017 |
Abfrontier |
50 ul |
EUR 400.8 |
Description: Mouse polyclonal to Integrin alpha E |
anti-Integrin alpha 6 |
YF-PA12761 |
Abfrontier |
50 ul |
EUR 435.6 |
Description: Mouse polyclonal to Integrin alpha 6 |
anti-Integrin alpha 6 |
YF-PA12762 |
Abfrontier |
100 ug |
EUR 483.6 |
Description: Rabbit polyclonal to Integrin alpha 6 |
anti-Integrin alpha 1 |
YF-PA12784 |
Abfrontier |
50 ug |
EUR 435.6 |
Description: Mouse polyclonal to Integrin alpha 1 |
anti-Integrin alpha 4 |
YF-PA12785 |
Abfrontier |
100 ug |
EUR 483.6 |
Description: Rabbit polyclonal to Integrin alpha 4 |
anti-Integrin alpha V |
YF-PA12786 |
Abfrontier |
50 ul |
EUR 435.6 |
Description: Mouse polyclonal to Integrin alpha V |
However, additional progresses of stem cell scientific investigation and its attainable software are restricted owing to the expense of economic LIF. Right here we launched a easy, sensible and excessive stage expression of MBP-mouse LIF by Escherichia coli system which was bioactive.
Rat Leukemia Inhibitory Issue (LIF) ELISA Package
The mLIF cDNA was inserted into vector of pMAL-C2X with a purpose to generate N-terminal MBP-mLIF recombinant proteins within the cytoplasm of Escherichia coli. MBP-mLIF as a soluble kind was expressed. One-step purification by gravitational affinity chromatography was achieved to amass excessive purity (>92%) MBP-mLIF. The MBP-mLIF merchandise particularly suppressed the expansion of M1cells in a dose-dependent sample. MBP-mLIF additionally was proved the power to keep up the pluripotency of iPSCs.
These outcomes revealed that the N-end MBP tags of the MBP-mLIF didn’t hinder mLIF bioactivity. This methodology to generate recombinant MBP-mLIF is an easy, sensible, economical and user-friendly protocol.