Effect on Mouse Leukemia Inhibitory Factor

Immunization in opposition to leukemia inhibitory issue and its receptor suppresses tumor formation of breast most cancers initiating cells in BALB/c mouse

• Immunotherapy is a promising method for particular focusing on of most cancers cells. Leukemia inhibitory issue (LIF) regulates a number of options of cancers and most cancers stem cells (CSCs) by binding to LIF receptor (LIFR). On this research, we investigated the consensus of LIF and LIFR immunization on the expansion of mouse mammary tumors. For this objective, mouse LIF and LIFR had been designed as truncated proteins, expressed in E. coli after which injected to mice as particular person and blended antigens.

 

• The outcomes confirmed the manufacturing of neutralizing antibodies and secretion of interferon-γ and interleukin-2 in response to immunization. In proceed, the immunized mice had been subjected for tumor formation problem by inoculation of the breast CSCs derived from MC4-L2 cells.

 

• Improvement of the breast tumors was noticed in all of the management mice, whereas the tumors appeared in 75% of animals within the LIF group. LIFR injection, individually or together with LIF, strongly inhibited the tumor development to solely 25% of the mice. Furthermore, a delay in tumor look was noticed within the immunized mice in comparison with the controls.

 

• Immunostaining of the tumor sections confirmed the expression of LIF and LIFR. In conclusion, LIF and LIFR is likely to be efficient targets for immunotherapy of the tumors that specific these proteins.

 

• Earlier research have proven that some inflammatory cytokines promote the expression of corticotropin-releasing hormone (CRH) in trophoblasts throughout being pregnant and that placental CRH might induce the manufacturing of adrenocorticotropic hormone (ACTH) in people.

 

• Nevertheless, whether or not the identical is true in rodent placenta stays unclear. On this research, we examined the impact of pro-inflammatory cytokine LIF on the induction of CRH in mouse trophoblast stem cells (mTSCs). Throughout differentiation, the CRH ranges in mTSCs progressively elevated.

Mouse Leukemia Inhibitory Issue (LIF) ELISA Package

• On days three and 5 after LIF supplementation, Crh expression within the differentiated mTSCs was considerably elevated with LIF remedy than these with out LIF remedy. Furthermore, the CRH focus within the tradition media elevated. Thereafter, we examined the contribution of the downstream pathways of LIF to CRH induction in differentiated mTSCs.

 

• The LIF-induced upregulation of CRH was attenuated by inhibition of PI3K/AKT and MAPK phosphorylation however not by inhibition of JAK/STAT3. Due to this fact, in mTSCs, LIF elevated Crh expression by activation of the PI3K/AKT and MAPK pathways however not by the JAK/STAT3 pathway. The current research means that mTSC is a perfect in vitro mannequin for finding out regulation and performance of placental CRH.

Bovine Leukemia Inhibitory Issue (LIF) ELISA Package

anti- LIF antibody
FNab09804	FN Test	100µg	EUR 658.5
Description: Antibody raised against LIF

anti- LIF antibody
LSMab09804	Lifescience Market	100 ug	EUR 463.2

Mouse LIF ELISA Kit
EML0014	Abclonal	96Tests	EUR 625.2

Mouse LIF shRNA Plasmid
20-abx971321	Abbexa	EUR 961.20 EUR 1345.20	150 µg 300 µg

Recombinant Mouse LIF Protein
R01400-1	BosterBio	100ug/vial	EUR 352.8

ELISA kit for Mouse LIF
EK5250	SAB	96 tests	EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse LIF in samples from serum, plasma, tissue homogenates and other biological fluids.

Mouse LIF PicoKine ELISA Kit
EK0580	BosterBio	96 wells	EUR 510
Description: For quantitative detection of mouse LIF in cell culture supernates, serum and plasma (heparin, EDTA).

Lif ORF Vector (Mouse) (pORF)
ORF049145	ABM	1.0 ug DNA	EUR 607.2

Lif ORF Vector (Mouse) (pORF)
ORF049146	ABM	1.0 ug DNA	EUR 607.2

LIF ELISA Kit (Mouse) (OKBB00760)
OKBB00760	Aviva Systems Biology	96 Wells	EUR 606
Description: Description of target: Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The LIF was mapped gene to 22q11-q12.2 by Southern analysis of a series of mouse/human somatic cell hybrids and by in situ hybridization to the chromosomes of 2 normal males and some individuals with chromosomal rearrangements. The gene maps between the Philadelphia translocation BCR1 and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: <10pg/ml

LIF ELISA Kit (Mouse) (OKEH04045)
OKEH04045	Aviva Systems Biology	96 Wells	EUR 652.8
Description: Description of target: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.039 ng/mL

LIF, Mouse Recombinant , 1MIU/ml
L4500-001	GenDepot	1ml	EUR 406.8

LIF, Mouse Recombinant , 1MIU/ml
L4500-005	GenDepot	5x1ml	EUR 1431.6

LIF, Mouse Recombinant , 10MIU/ml
L4501-001	GenDepot	1ml	EUR 1744.8

Nori® Mouse LIF ELISA Kit
GR117058	Genorise Scientific	96-well	EUR 461

Human CellExp? LIF, mouse recombinant
7268-10	Biovision	each	EUR 444

Leukemia Inhibitory Factor (LIF), mouse
PR16102	Neuromics	5 ug	EUR 522

Lif sgRNA CRISPR Lentivector set (Mouse)
K3872501	ABM	3 x 1.0 ug	EUR 406.8

LIF Antibody
25283-100ul	SAB	100ul	EUR 468

LIF Antibody
6245-002mg	ProSci	0.02 mg	EUR 206.18
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.

LIF Antibody
6245-01mg	ProSci	0.1 mg	EUR 523.7
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.

LIF antibody
70R-12299	Fitzgerald	100 ug	EUR 483.6
Description: Rabbit polyclonal LIF antibody

LIF antibody
70R-14100	Fitzgerald	100 ug	EUR 386.4
Description: Affinity purified Rabbit polyclonal LIF antibody

LIF antibody
70R-6230	Fitzgerald	50 ug	EUR 560.4
Description: Rabbit polyclonal LIF antibody

LIF antibody
70R-51295	Fitzgerald	100 ul	EUR 292.8
Description: Purified Polyclonal LIF antibody

LIF Antibody
36950-100ul	SAB	100ul	EUR 302.4

LIF Antibody
5216-100	Biovision	each	EUR 379.2

LIF Antibody
5216-30T	Biovision	each	EUR 175.2

LIF Antibody
1-CSB-PA004761	Cusabio	EUR 266.40 EUR 234.00	100ug 50ug
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000

LIF Antibody
1-CSB-PA198481	Cusabio	EUR 380.40 EUR 292.80	100ul 50ul
Description: A polyclonal antibody against LIF. Recognizes LIF from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:10000, IHC:1:25-1:100

LIF Antibody
DF13730	Affbiotech	100ul	EUR 420

LIF Antibody
1-CSB-PA012928ESR2HU	Cusabio	EUR 266.40 EUR 402.00	100ul 50ul
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: ELISA

LIF Antibody
F49998-0.08ML	NSJ Bioreagents	0.08 ml	EUR 140.25
Description: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.

LIF Antibody
F49998-0.4ML	NSJ Bioreagents	0.4 ml	EUR 322.15
Description: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.

LIF antibody
PAab09804	Lifescience Market	100 ug	EUR 463.2

LIF Antibody
R36251-100UG	NSJ Bioreagents	100 ug	EUR 339.15
Description: Additional name(s) for this target protein: Leukemia inhibitory factor

LIF Antibody
R30639	NSJ Bioreagents	100 ug	EUR 356.15
Description: LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice. It is produced in high amounts by the human endometrium and the trophoblast itself, and its receptors are present on cytotrophoblast cells. LIF could thus play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface. The gene maps to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2.

LIF Antibody
R31467	NSJ Bioreagents	100 ug	EUR 356.15
Description: LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice. This gene is mapped to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF is produced in high amounts by the human endometrium and the trophoblast itself, and LIF receptors are present on cytotrophoblast cells. It could, thus, play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface.

Leukemia Inhibitory Factor (LIF) Polyclonal Antibody (Mouse)
4-PAA085Mu01	Cloud-Clone	EUR 283.20 EUR 2805.60 EUR 703.20 EUR 352.80 EUR 250.80	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Leukemia Inhibitory Factor (LIF)

LIF, Leukemia inhibitory factor, murine (mouse)
RC234-18	Bio Basic	5ug	EUR 125.26

Mouse Leukemia Inhibitory Factor (LIF) Protein
abx262959-1mg	Abbexa	1 mg	EUR 3801.6

Mouse Leukemia Inhibitory Factor (LIF) Protein
abx262959-3x10g	Abbexa	3x10 µg	EUR 393.6

Mouse Leukemia Inhibitory Factor (LIF) Protein
abx262959-3x2g	Abbexa	3x2 µg	EUR 276

Mouse Leukemia Inhibitory Factor (LIF) Protein
20-abx067761	Abbexa	EUR 794.40 EUR 326.40 EUR 2414.40 EUR 944.40 EUR 577.20	100 ug 10 ug 1 mg 200 ug 50 ug

anti-LIF
YF-PA12952	Abfrontier	50 ug	EUR 435.6
Description: Mouse polyclonal to LIF

LIF Protein Vector (Mouse) (pPM-C-HA)
PV196580	ABM	500 ng	EUR 723.6

LIF Protein Vector (Mouse) (pPM-C-HA)
PV196584	ABM	500 ng	EUR 723.6

Recombinant Mouse Leukemia Inhibitory Factor/lIF
C690-10ug	Novoprotein	10ug	EUR 175.2
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

Recombinant Mouse Leukemia Inhibitory Factor/lIF
C690-1mg	Novoprotein	1mg	EUR 2739.6
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

Recombinant Mouse Leukemia Inhibitory Factor/lIF
C690-500ug	Novoprotein	500ug	EUR 1935.6
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

Recombinant Mouse Leukemia Inhibitory Factor/lIF
C690-50ug	Novoprotein	50ug	EUR 363.6
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

LIF Protein Vector (Mouse) (pPB-C-His)
PV196578	ABM	500 ng	EUR 723.6

LIF Protein Vector (Mouse) (pPB-N-His)
PV196579	ABM	500 ng	EUR 723.6

LIF Protein Vector (Mouse) (pPM-C-His)
PV196581	ABM	500 ng	EUR 723.6

LIF Protein Vector (Mouse) (pPB-C-His)
PV196582	ABM	500 ng	EUR 723.6

LIF Protein Vector (Mouse) (pPB-N-His)
PV196583	ABM	500 ng	EUR 723.6

LIF Protein Vector (Mouse) (pPM-C-His)
PV196585	ABM	500 ng	EUR 723.6

Mouse Lif/ Leukemia inhibitory factor ELISA Kit
E1656Mo	Sunlong	1 Kit	EUR 655.2

Mouse Lif(Leukemia inhibitory factor) ELISA Kit
EM0237	FN Test	96T	EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 46.9pg/ml

Mouse LIF Protein (Ser 24-Phe 203) [His]
VAng-1946Lsx-20g	Creative Biolabs	20 µg	EUR 588
Description: Mouse LIF protein, His tag, expressed in human 293 cells. (Uniprot ID: P09056)

Mouse LIF Protein (Ser 24-Phe 203) [His]
VAng-1946Lsx-50g	Creative Biolabs	50 µg	EUR 1050
Description: Mouse LIF protein, His tag, expressed in human 293 cells. (Uniprot ID: P09056)

Leukemia Inhibitory Factor (LIF) Polyclonal Antibody (Mouse), PE
4-PAA085Mu01-PE	Cloud-Clone	EUR 339.60 EUR 2942.40 EUR 843.60 EUR 423.60 EUR 226.80	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Leukemia Inhibitory Factor (LIF). This antibody is labeled with PE.

Recombinant Mouse LIF Protein, Untagged, E.coli-1mg
QP4235-1mg	EnQuireBio	1mg	EUR 3189.6

Recombinant Mouse LIF Protein, Untagged, E.coli-5ug
QP4235-5ug	EnQuireBio	5ug	EUR 163.2

Leukemia Inhibitory Factor (LIF) Polyclonal Antibody (Mouse), APC
4-PAA085Mu01-APC	Cloud-Clone	EUR 394.80 EUR 3649.20 EUR 1024.80 EUR 499.20 EUR 254.40	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Leukemia Inhibitory Factor (LIF). This antibody is labeled with APC.

Leukemia Inhibitory Factor (LIF) Polyclonal Antibody (Mouse), Cy3
4-PAA085Mu01-Cy3	Cloud-Clone	EUR 476.40 EUR 4815.60 EUR 1316.40 EUR 615.60 EUR 289.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Leukemia Inhibitory Factor (LIF). This antibody is labeled with Cy3.

Leukemia Inhibitory Factor (LIF) Polyclonal Antibody (Mouse), HRP
4-PAA085Mu01-HRP	Cloud-Clone	EUR 362.40 EUR 3182.40 EUR 907.20 EUR 452.40 EUR 240.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Leukemia Inhibitory Factor (LIF). This antibody is labeled with HRP.

Recombinant Mouse LIF Protein, Untagged, E.coli-20ug
QP4235-20ug	EnQuireBio	20ug	EUR 284.4

Leukemia Inhibitory Factor (LIF) Polyclonal Antibody (Mouse), FITC
4-PAA085Mu01-FITC	Cloud-Clone	EUR 339.60 EUR 2942.40 EUR 843.60 EUR 423.60 EUR 226.80	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Leukemia Inhibitory Factor (LIF). This antibody is labeled with FITC.

Recombinant Mouse LIF Protein, Untagged, E.coli-100ug
QP4235-100ug	EnQuireBio	100ug	EUR 729.6

Recombinant Mouse LIF Protein, Untagged, E.coli-500ug
QP4235-500ug	EnQuireBio	500ug	EUR 1992

Lif sgRNA CRISPR Lentivector (Mouse) (Target 1)
K3872502	ABM	1.0 ug DNA	EUR 184.8

Lif sgRNA CRISPR Lentivector (Mouse) (Target 2)
K3872503	ABM	1.0 ug DNA	EUR 184.8

Lif sgRNA CRISPR Lentivector (Mouse) (Target 3)
K3872504	ABM	1.0 ug DNA	EUR 184.8

Mouse Leukemia Inhibitory Factor (LIF) CLIA Kit
abx195395-96tests	Abbexa	96 tests	EUR 990

Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit
abx254611-96tests	Abbexa	96 tests	EUR 904.8

Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit
abx574566-96tests	Abbexa	96 tests	EUR 904.8

Mouse Leukemia inhibitory factor, LIF ELISA kit
CSB-E04652m-24T	Cusabio	1 plate of 24 wells	EUR 198
Description: Quantitativesandwich ELISA kit for measuring Mouse Leukemia inhibitory factor, LIF in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse Leukemia inhibitory factor, LIF ELISA kit
1-CSB-E04652m	Cusabio	EUR 1135.20 EUR 6938.40 EUR 3672.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Mouse Leukemia inhibitory factor, LIF in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse Leukemia inhibitory factor,LIF ELISA kit
CN-02441M1	ChemNorm	96T	EUR 560.4

Mouse Leukemia inhibitory factor,LIF ELISA kit
CN-02441M2	ChemNorm	48T	EUR 379.2

Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-Mu-48T	DL Develop	48T	EUR 586.8
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-Mu-96T	DL Develop	96T	EUR 762
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Leukemia inhibitory factor(LIF)ELISA Kit
GA-E0658MS-48T	GenAsia Biotech	48T	EUR 403.2

Mouse Leukemia inhibitory factor(LIF)ELISA Kit
GA-E0658MS-96T	GenAsia Biotech	96T	EUR 640.8

Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Mu-48Tests	Reddot Biotech	48 Tests	EUR 613.2

Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Mu-96Tests	Reddot Biotech	96 Tests	EUR 850.8

Mouse leukemia inhibitory factor(LIF)ELISA Kit
QY-E20907	Qayee Biotechnology	96T	EUR 433.2

Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Mu-48Tests	Reddot Biotech	48 Tests	EUR 586.8

Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Mu-96Tests	Reddot Biotech	96 Tests	EUR 812.4

 

Canine Leukemia Inhibitory Issue (LIF) ELISA Package

 

Manufacturing of soluble bioactive mouse leukemia inhibitory issue from Escherichia coli utilizing MBP tag.

Embryonic stem cells and induced pluripotent stem cells rely on one in every of cytokines referred to as leukemia inhibitory issue (LIF) to retain their undifferentiated state and pluripotency.

Integrin alpha 5 Antibody
49269-100ul	SAB	100ul	EUR 399.6

Integrin alpha 5 Antibody
49269-50ul	SAB	50ul	EUR 286.8

Integrin alpha 5 Antibody
F41992-0.4ML	NSJ Bioreagents	0.4 ml	EUR 322.15
Description: The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq].

Integrin alpha 5 Antibody
F49220-0.4ML	NSJ Bioreagents	0.4 ml	EUR 322.15
Description: The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq].

Integrin alpha 5 Antibody
R31667	NSJ Bioreagents	100 ug	EUR 356.15
Description: Integrin alpha-5, also known as FNRA or VLA5A, is a protein that in humans is encoded by the ITGA5 gene. The product of this gene belongs to the integrin alpha chain family. Integrins are integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

Human Integrin Alpha 5 (ITGa5) Protein
20-abx067272	Abbexa	EUR 794.40 EUR 326.40 EUR 2448.00 EUR 944.40 EUR 577.20	100 ug 10 ug 1 mg 200 ug 50 ug

ELISA kit for Human Integrin alpha-5
EK4913	SAB	96 tests	EUR 804
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Integrin alpha-5 in samples from serum, plasma, tissue homogenates and other biological fluids.

Human Integrin alpha-5(ITGA5) Elisa kit
CSB-E17350h-24T	Cusabio	1 plate of 24 wells	EUR 198
Description: Quantitativesandwich ELISA kit for measuring Human Integrin alpha-5 (ITGA5) in samples from serum, plasma, cell culture supernates, urine, cerebrospinalfluid (CSF). A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Integrin alpha-5(ITGA5) Elisa kit
1-CSB-E17350h	Cusabio	EUR 1080.00 EUR 6571.20 EUR 3480.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Integrin alpha-5(ITGA5) in samples from serum, plasma, cell culture supernates, urine, cerebrospinalfluid(CSF). Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Integrin alpha-5(ITGA5) Elisa kit
YLA4349HU-48T	Shanghai YL Biotech	48T	EUR 435

Human Integrin alpha-5(ITGA5) Elisa kit
YLA4349HU-96T	Shanghai YL Biotech	96T	EUR 562.5

Human Integrin Alpha 5 (ITGa5) CLIA Kit
20-abx492595	Abbexa	EUR 9567.60 EUR 5095.20 EUR 1177.20	10 × 96 tests 5 × 96 tests 96 tests

Human ITGA5(Integrin alpha-5) ELISA Kit
EH2445	FN Test	96T	EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Human ITGA5/ Integrin alpha-5 ELISA Kit
E1342Hu	Sunlong	1 Kit	EUR 726

Human Integrin Alpha 5 (ITGa5) ELISA Kit
20-abx151987	Abbexa	EUR 8534.40 EUR 4550.40 EUR 1054.80	10 × 96 tests 5 × 96 tests 96 tests

Human Integrin Alpha 5 (ITGA5) ELISA Kit
abx571172-96tests	Abbexa	96 tests	EUR 886.8

Human Integrin Alpha 5 (ITGa5) ELISA Kit
DLR-ITGa5-Hu-48T	DL Develop	48T	EUR 597.6
Description: A sandwich quantitative ELISA assay kit for detection of Human Integrin Alpha 5 (ITGa5) in samples from tissue homogenates, cell lysates or other biological fluids.

Human Integrin Alpha 5 (ITGa5) ELISA Kit
DLR-ITGa5-Hu-96T	DL Develop	96T	EUR 776.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Integrin Alpha 5 (ITGa5) in samples from tissue homogenates, cell lysates or other biological fluids.

Human Integrin alpha- 5, ITGA5 ELISA KIT
ELI-07691h	Lifescience Market	96 Tests	EUR 988.8

Human Integrin Alpha 5 (ITGa5) ELISA Kit
RDR-ITGa5-Hu-48Tests	Reddot Biotech	48 Tests	EUR 626.4

Human Integrin Alpha 5 (ITGa5) ELISA Kit
RDR-ITGa5-Hu-96Tests	Reddot Biotech	96 Tests	EUR 868.8

Human Integrin Alpha 5(ITGa5) ELISA Kit
QY-E05294	Qayee Biotechnology	96T	EUR 472.8

Human Integrin Alpha 5 (ITGa5) ELISA Kit
RD-ITGa5-Hu-48Tests	Reddot Biotech	48 Tests	EUR 600

Human Integrin Alpha 5 (ITGa5) ELISA Kit
RD-ITGa5-Hu-96Tests	Reddot Biotech	96 Tests	EUR 830.4

Human Integrin Alpha 5 (ITGa5) ELISA Kit
SEB287Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 5402.92
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.

Human Integrin Alpha 5 (ITGa5) ELISA Kit
SEB287Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 550.13
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.

Human Integrin Alpha 5 (ITGa5) ELISA Kit
SEB287Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 734.46
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.

Human Integrin Alpha 5 (ITGa5) ELISA Kit
SEB287Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2945.08
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.

Human Integrin Alpha 5 (ITGa5) ELISA Kit
4-SEB287Hu	Cloud-Clone	EUR 5463.60 EUR 2886.00 EUR 735.60	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Integrin Alpha 5 (ITGa5) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Integrin Alpha 5 (ITGa5) ELISA Kit
RD-ITGa5-Hu-48T	Reddot Biotech	48T	EUR 432.3
Description: tissue homogenates, cell lysates and other biological fluids.

Human Integrin Alpha 5 (ITGa5) ELISA Kit
RD-ITGa5-Hu-96T	Reddot Biotech	96T	EUR 617.5
Description: tissue homogenates, cell lysates and other biological fluids.

Human Integrin Alpha 5 (ITGa5) ELISA Kit
RDR-ITGa5-Hu-48T	Reddot Biotech	48T	EUR 453.92
Description: tissue homogenates, cell lysates and other biological fluids.

Human Integrin Alpha 5 (ITGa5) ELISA Kit
RDR-ITGa5-Hu-96T	Reddot Biotech	96T	EUR 648.38
Description: tissue homogenates, cell lysates and other biological fluids.

Integrin Alpha 5 (ITGa5) Polyclonal Antibody (Human)
4-PAB287Hu01	Cloud-Clone	EUR 286.80 EUR 2869.20 EUR 717.60 EUR 358.80 EUR 253.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Integrin Alpha 5 (ITGa5)

Integrin Alpha 5 (ITGa5) Antibody
20-abx101634	Abbexa	EUR 493.20 EUR 159.60 EUR 1378.80 EUR 678.00 EUR 376.80	100 ug 10 ug 1 mg 200 ug 50 ug

Integrin Alpha 5 (ITGa5) Antibody
20-abx101635	Abbexa	EUR 560.40 EUR 159.60 EUR 1612.80 EUR 760.80 EUR 427.20	100 ug 10 ug 1 mg 200 ug 50 ug

Integrin Alpha 5 (ITGa5) Antibody
20-abx172956	Abbexa	EUR 978.00 EUR 510.00	1 mg 200 ug

Integrin Alpha 5 (ITGa5) Antibody
20-abx179024	Abbexa	EUR 2013.60 EUR 927.60	1 mg 200 ug

Integrin alpha 5 (CD49e) Antibody
abx216264-100ug	Abbexa	100 ug	EUR 526.8

Integrin Alpha 5 (ITGA5) Antibody
20-abx325454	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Integrin Alpha 5 (ITGA5) Antibody
20-abx327245	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Integrin Alpha 5 (ITGA5) Antibody
20-abx113185	Abbexa	EUR 878.40 EUR 477.60	150 ul 50 ul

Integrin Alpha 5 (ITGA5) Antibody
abx026655-400ul	Abbexa	400 ul	EUR 627.6

Integrin Alpha 5 (ITGA5) Antibody
abx026655-80l	Abbexa	80 µl	EUR 343.2

Integrin Alpha 5 (ITGa5) Antibody
20-abx129339	Abbexa	EUR 510.00 EUR 159.60 EUR 1412.40 EUR 693.60 EUR 393.60	100 ug 10 ug 1 mg 200 ug 50 ug

Integrin Alpha 5 (ITGA5) Antibody
20-abx323564	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Integrin Alpha 5 (ITGA5) Antibody
20-abx338742	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Integrin Alpha 5 (ITGA5) Antibody
20-abx006811	Abbexa	EUR 493.20 EUR 710.40 EUR 218.40 EUR 376.80	100 ul 200 ul 20 ul 50 ul

Integrin Alpha 5 (ITGA5) Antibody
20-abx000873	Abbexa	EUR 493.20 EUR 710.40	100 ul 200 ul

Integrin Alpha 5 (ITGA5) Antibody
abx011678-100ul	Abbexa	100 ul	EUR 493.2

Integrin alpha 5 Antibody (ITGA5)
F41992-0.08ML	NSJ Bioreagents	0.08 ml	EUR 140.25
Description: The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq].

Integrin alpha 5 Antibody (ITGA5)
F49220-0.08ML	NSJ Bioreagents	0.08 ml	EUR 140.25
Description: The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq].

Integrin alpha 5 Antibody / ITGA5
RQ4096	NSJ Bioreagents	100 ug	EUR 356.15
Description: Integrin alpha-5, also known as FNRA or VLA5A, is a protein that in humans is encoded by the ITGA5 gene. The product of this gene belongs to the integrin alpha chain family. Integrins are integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

Integrin alpha 5 Antibody / ITGA5
RQ5245	NSJ Bioreagents	100ul	EUR 356.15
Description: The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes. [RefSeq]

Mouse Anti Human Integrin Alpha V Beta 5 Monoclonal Antibody
CABT-49626MH	Creative Diagnostics	0.1 mg	EUR 1650

Integrin alpha 5 Conjugated Antibody
C49269	SAB	100ul	EUR 476.4

Integrin alpha 5 Rabbit mAb
A19069-100ul	Abclonal	100 ul	EUR 492

Integrin alpha 5 Rabbit mAb
A19069-200ul	Abclonal	200 ul	EUR 685.2

Integrin alpha 5 Rabbit mAb
A19069-20ul	Abclonal	20 ul	EUR 265.2

Integrin alpha 5 Rabbit mAb
A19069-50ul	Abclonal	50 ul	EUR 344.4

Integrin Alpha 5 (ITGa5) Polyclonal Antibody (Human), PE
4-PAB287Hu01-PE	Cloud-Clone	EUR 344.40 EUR 3012.00 EUR 860.40 EUR 430.80 EUR 230.40	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Integrin Alpha 5 (ITGa5). This antibody is labeled with PE.

Integrin Alpha 5 (ITGa5) Polyclonal Antibody (Human), APC
4-PAB287Hu01-APC	Cloud-Clone	EUR 399.60 EUR 3735.60 EUR 1046.40 EUR 507.60 EUR 258.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Integrin Alpha 5 (ITGa5). This antibody is labeled with APC.

Integrin Alpha 5 (ITGa5) Polyclonal Antibody (Human), Cy3
4-PAB287Hu01-Cy3	Cloud-Clone	EUR 483.60 EUR 4930.80 EUR 1345.20 EUR 627.60 EUR 294.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Integrin Alpha 5 (ITGa5). This antibody is labeled with Cy3.

Integrin Alpha 5 (ITGa5) Polyclonal Antibody (Human), HRP
4-PAB287Hu01-HRP	Cloud-Clone	EUR 366.00 EUR 3256.80 EUR 926.40 EUR 459.60 EUR 243.60	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Integrin Alpha 5 (ITGa5). This antibody is labeled with HRP.

Recombinant Antibody to Integrin Alpha 5 (ITGa5) (Human)
4-RAB287Hu21	Cloud-Clone	EUR 406.80 EUR 4754.40 EUR 1146.00 EUR 530.40 EUR 303.60	100ul 10ml 1ml 200ul 20ul
Description: A Recombinant antibody against Human Integrin Alpha 5 (ITGa5)

ELISA kit for Human ITGa5 (Integrin Alpha 5)
ELK3746	ELK Biotech	1 plate of 96 wells	EUR 518.4
Description: A sandwich ELISA kit for detection of Integrin Alpha 5 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Integrin Alpha 5 (ITGa5) Polyclonal Antibody (Human), FITC
4-PAB287Hu01-FITC	Cloud-Clone	EUR 344.40 EUR 3012.00 EUR 860.40 EUR 430.80 EUR 230.40	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Integrin Alpha 5 (ITGa5). This antibody is labeled with FITC.

Integrin Alpha 5 (ITGA5) Antibody (HRP)
20-abx336126	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Integrin Alpha 5 (ITGA5) Antibody (FITC)
20-abx336127	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Integrin Alpha 5 (ITGA5) Antibody (FITC)
abx413989-01mg	Abbexa	0.1 mg	EUR 610.8

anti- Integrin alpha-1 antibody
FNab04343	FN Test	100µg	EUR 702
Description: Antibody raised against Integrin alpha-1

anti- Integrin alpha-3 antibody
FNab04344	FN Test	100µg	EUR 702
Description: Antibody raised against Integrin alpha-3

anti- Integrin alpha-3 antibody
FNab04345	FN Test	100µg	EUR 702
Description: Antibody raised against Integrin alpha-3

anti- Integrin alpha-4 antibody
FNab04346	FN Test	100µg	EUR 606.3
Description: Antibody raised against Integrin alpha-4

anti- Integrin alpha-9 antibody
FNab04348	FN Test	100µg	EUR 606.3
Description: Antibody raised against Integrin alpha-9

Integrin Alpha 5 (ITGA5) Antibody (Biotin)
20-abx336128	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Integrin alpha-5 Recombinant Protein
91-433	ProSci	0.05 mg	EUR 651.3
Description: Integrin alpha-5 belongs to the Integrin alpha chain family and contains 7 FG-GAP repeats. Integrin alpha-5 joins with Integrin- beta 1 to form a fibronectin and laminin receptor which recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. It is expressed on fibroblasts, endothelial cells, peripheral T cells and platelets. Integrin alpha-5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains. In addition to adhesion, ITGA5 participates in cell-surface mediated signalling.

Recombinant Integrin Alpha 5 (ITGa5)
4-RPB287Bo01	Cloud-Clone	EUR 668.35 EUR 302.40 EUR 2176.32 EUR 805.44 EUR 1490.88 EUR 523.20 EUR 5260.80	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Bovine Integrin Alpha 5 expressed in: E.coli

Recombinant Integrin Alpha 5 (ITGa5)
4-RPB287Hu01	Cloud-Clone	EUR 571.58 EUR 276.00 EUR 1813.44 EUR 684.48 EUR 1248.96 EUR 458.40 EUR 4353.60	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Integrin Alpha 5 expressed in: E.coli

Recombinant Integrin Alpha 5 (ITGa5)
4-RPB287Mu01	Cloud-Clone	EUR 571.58 EUR 276.00 EUR 1813.44 EUR 684.48 EUR 1248.96 EUR 458.40 EUR 4353.60	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Mouse Integrin Alpha 5 expressed in: E.coli

Human Integrin alpha-2 (ITGA2)
1-CSB-YP011864HU	Cusabio	EUR 516.00 EUR 280.80 EUR 1809.60 EUR 770.40 EUR 1210.80 EUR 349.20	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Integrin alpha-2(ITGA2),partial expressed in Yeast

Human Integrin alpha-2 (ITGA2)
1-CSB-EP011864HU	Cusabio	EUR 456.00 EUR 256.80 EUR 1570.80 EUR 672.00 EUR 1047.60 EUR 314.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Integrin alpha-2(ITGA2) ,partial expressed in E.coli

Recombinant Antibody to Integrin Alpha 5 (ITGa5) (Human), PE
4-RAB287Hu21-PE	Cloud-Clone	EUR 488.40 EUR 5068.80 EUR 1375.20 EUR 636.00 EUR 291.60	100ul 10ml 1ml 200ul 20ul
Description: A Recombinant antibody against Human Integrin Alpha 5 (ITGa5). This antibody is labeled with PE.

Recombinant Antibody to Integrin Alpha 5 (ITGa5) (Human), APC
4-RAB287Hu21-APC	Cloud-Clone	EUR 580.80 EUR 6306.00 EUR 1688.40 EUR 765.60 EUR 333.60	100ul 10ml 1ml 200ul 20ul
Description: A Recombinant antibody against Human Integrin Alpha 5 (ITGa5). This antibody is labeled with APC.

Recombinant Antibody to Integrin Alpha 5 (ITGa5) (Human), Cy3
4-RAB287Hu21-Cy3	Cloud-Clone	EUR 723.60 EUR 8358.00 EUR 2202.00 EUR 970.80 EUR 396.00	100ul 10ml 1ml 200ul 20ul
Description: A Recombinant antibody against Human Integrin Alpha 5 (ITGa5). This antibody is labeled with Cy3.

Recombinant Antibody to Integrin Alpha 5 (ITGa5) (Human), HRP
4-RAB287Hu21-HRP	Cloud-Clone	EUR 523.20 EUR 5485.20 EUR 1483.20 EUR 682.80 EUR 309.60	100ul 10ml 1ml 200ul 20ul
Description: A Recombinant antibody against Human Integrin Alpha 5 (ITGa5). This antibody is labeled with HRP.

Recombinant Antibody to Integrin Alpha 5 (ITGa5) (Human), FITC
4-RAB287Hu21-FITC	Cloud-Clone	EUR 488.40 EUR 5068.80 EUR 1375.20 EUR 636.00 EUR 291.60	100ul 10ml 1ml 200ul 20ul
Description: A Recombinant antibody against Human Integrin Alpha 5 (ITGa5). This antibody is labeled with FITC.

Integrin Alpha 5 (ITGa5) Polyclonal Antibody (Human), Biotinylated
4-PAB287Hu01-Biotin	Cloud-Clone	EUR 363.60 EUR 2809.20 EUR 836.40 EUR 442.80 EUR 259.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Integrin Alpha 5 (ITGa5). This antibody is labeled with Biotin.

Human Integrin alpha-V (ITGAV)
1-CSB-YP011877HU	Cusabio	EUR 516.00 EUR 280.80 EUR 1809.60 EUR 770.40 EUR 1210.80 EUR 349.20	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Integrin alpha-V(ITGAV),partial expressed in Yeast

Integrin Alpha 5 (ITGa5) Polyclonal Antibody (Human), APC-Cy7
4-PAB287Hu01-APC-Cy7	Cloud-Clone	EUR 656.40 EUR 7327.20 EUR 1948.80 EUR 872.40 EUR 372.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Integrin Alpha 5 (ITGa5). This antibody is labeled with APC-Cy7.

Cow Integrin Alpha 5 (ITGa5) Protein
20-abx067274	Abbexa	EUR 927.60 EUR 360.00 EUR 2932.80 EUR 1111.20 EUR 661.20	100 ug 10 ug 1 mg 200 ug 50 ug

Recombinant human Integrin alpha-10
P1321	FN Test	100ug	Ask for price
Description: Recombinant protein for human Integrin alpha-10

Human Integrin alpha-IIb (ITGA2B)
1-CSB-YP011865HU	Cusabio	EUR 516.00 EUR 280.80 EUR 1809.60 EUR 770.40 EUR 1210.80 EUR 349.20	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Integrin alpha-IIb(ITGA2B),partial expressed in Yeast

However, additional progresses of stem cell scientific investigation and its attainable software are restricted owing to the expense of economic LIF. Right here we launched a easy, sensible and excessive stage expression of MBP-mouse LIF by Escherichia coli system which was bioactive.

Rat Leukemia Inhibitory Issue (LIF) ELISA Package

The mLIF cDNA was inserted into vector of pMAL-C2X with a purpose to generate N-terminal MBP-mLIF recombinant proteins within the cytoplasm of Escherichia coli. MBP-mLIF as a soluble kind was expressed. One-step purification by gravitational affinity chromatography was achieved to amass excessive purity (>92%) MBP-mLIF. The MBP-mLIF merchandise particularly suppressed the expansion of M1cells in a dose-dependent sample. MBP-mLIF additionally was proved the power to keep up the pluripotency of iPSCs.

 

These outcomes revealed that the N-end MBP tags of the MBP-mLIF didn’t hinder mLIF bioactivity. This methodology to generate recombinant MBP-mLIF is an easy, sensible, economical and user-friendly protocol.