Immunization in opposition to leukemia inhibitory issue and its receptor suppresses tumor formation of breast most cancers initiating cells in BALB/c mouse
- Immunotherapy is a promising method for particular focusing on of most cancers cells. Leukemia inhibitory issue (LIF) regulates a number of options of cancers and most cancers stem cells (CSCs) by binding to LIF receptor (LIFR). On this research, we investigated the consensus of LIF and LIFR immunization on the expansion of mouse mammary tumors. For this objective, mouse LIF and LIFR had been designed as truncated proteins, expressed in E. coli after which injected to mice as particular person and blended antigens.
- The outcomes confirmed the manufacturing of neutralizing antibodies and secretion of interferon-γ and interleukin-2 in response to immunization. In proceed, the immunized mice had been subjected for tumor formation problem by inoculation of the breast CSCs derived from MC4-L2 cells.
- Improvement of the breast tumors was noticed in all of the management mice, whereas the tumors appeared in 75% of animals within the LIF group. LIFR injection, individually or together with LIF, strongly inhibited the tumor development to solely 25% of the mice. Furthermore, a delay in tumor look was noticed within the immunized mice in comparison with the controls.
- Immunostaining of the tumor sections confirmed the expression of LIF and LIFR. In conclusion, LIF and LIFR is likely to be efficient targets for immunotherapy of the tumors that specific these proteins.
- Earlier research have proven that some inflammatory cytokines promote the expression of corticotropin-releasing hormone (CRH) in trophoblasts throughout being pregnant and that placental CRH might induce the manufacturing of adrenocorticotropic hormone (ACTH) in people.
- Nevertheless, whether or not the identical is true in rodent placenta stays unclear. On this research, we examined the impact of pro-inflammatory cytokine LIF on the induction of CRH in mouse trophoblast stem cells (mTSCs). Throughout differentiation, the CRH ranges in mTSCs progressively elevated.
Mouse Leukemia Inhibitory Issue (LIF) ELISA Package
- On days three and 5 after LIF supplementation, Crh expression within the differentiated mTSCs was considerably elevated with LIF remedy than these with out LIF remedy. Furthermore, the CRH focus within the tradition media elevated. Thereafter, we examined the contribution of the downstream pathways of LIF to CRH induction in differentiated mTSCs.
- The LIF-induced upregulation of CRH was attenuated by inhibition of PI3K/AKT and MAPK phosphorylation however not by inhibition of JAK/STAT3. Due to this fact, in mTSCs, LIF elevated Crh expression by activation of the PI3K/AKT and MAPK pathways however not by the JAK/STAT3 pathway. The current research means that mTSC is a perfect in vitro mannequin for finding out regulation and performance of placental CRH.
Bovine Leukemia Inhibitory Issue (LIF) ELISA Package
Mouse LIF |
MBS692107-5x001mg |
MyBiosource |
5x0.01mg |
EUR 1080 |
Mouse LIF |
MBS691665-5x005mg |
MyBiosource |
5x0.05mg |
EUR 2045 |
LIF,Mouse |
E34M066M |
EnoGene |
5 μg |
EUR 155 |
LIF, mouse |
MO16102 |
Neuromics |
100 ug |
EUR 494.1 |
LIF, Mouse |
MBS8575391-0005mg |
MyBiosource |
0.005mg |
EUR 245 |
LIF, Mouse |
MBS8575391-5x0005mg |
MyBiosource |
5x0.005mg |
EUR 940 |
Mouse Anti-Human LIF Monoclonal Antibody |
MBS592457-01mg |
MyBiosource |
0.1mg |
EUR 365 |
Mouse Anti-Human LIF Monoclonal Antibody |
MBS592457-05mg |
MyBiosource |
0.5mg |
EUR 530 |
Mouse Anti-Human LIF Monoclonal Antibody |
MBS592457-5x05mg |
MyBiosource |
5x0.5mg |
EUR 2140 |
Mouse Anti-Human LIF Monoclonal Antibody |
MBS592458-01mg |
MyBiosource |
0.1mg |
EUR 365 |
Mouse Anti-Human LIF Monoclonal Antibody |
MBS592458-05mg |
MyBiosource |
0.5mg |
EUR 530 |
Mouse Anti-Human LIF Monoclonal Antibody |
MBS592458-5x05mg |
MyBiosource |
5x0.5mg |
EUR 2140 |
Mouse Anti-Human LIF Monoclonal Antibody |
MBS592459-01mg |
MyBiosource |
0.1mg |
EUR 365 |
Mouse Anti-Human LIF Monoclonal Antibody |
MBS592459-05mg |
MyBiosource |
0.5mg |
EUR 530 |
Mouse Anti-Human LIF Monoclonal Antibody |
MBS592459-5x05mg |
MyBiosource |
5x0.5mg |
EUR 2140 |
Mouse LIF siRNA |
20-abx922546 |
Abbexa |
-
Ask for price
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|
|
|
Mouse LIF Protein |
E2PD201245 |
EnoGene |
20ug |
EUR 595 |
Mouse LIF Protein |
E40MOP1672 |
EnoGene |
20ug |
EUR 495 |
Mouse LIF Protein |
E40MOP2026 |
EnoGene |
20ug |
EUR 495 |
Mouse LIF Protein |
E40MOP2028 |
EnoGene |
20ug |
EUR 495 |
LIF, Mouse, Monoclonal Antibody, Mouse |
MBS555093-01mg |
MyBiosource |
0.1mg |
EUR 745 |
LIF, Mouse, Monoclonal Antibody, Mouse |
MBS555093-5x01mg |
MyBiosource |
5x0.1mg |
EUR 3210 |
LIF siRNA (Mouse) |
MBS8224619-15nmol |
MyBiosource |
15nmol |
EUR 405 |
LIF siRNA (Mouse) |
MBS8224619-30nmol |
MyBiosource |
30nmol |
EUR 565 |
LIF siRNA (Mouse) |
MBS8224619-5x30nmol |
MyBiosource |
5x30nmol |
EUR 2450 |
Recombinant Mouse LIF |
MBS4159936-001mg |
MyBiosource |
0.01mg |
EUR 230 |
Recombinant Mouse LIF |
MBS4159936-01mg |
MyBiosource |
0.1mg |
EUR 560 |
Recombinant Mouse LIF |
MBS4159936-1mg |
MyBiosource |
1mg |
EUR 1500 |
Recombinant Mouse LIF |
MBS4159936-5x1mg |
MyBiosource |
5x1mg |
EUR 6500 |
Recombinant Mouse LIF |
MBS7609292-005mg |
MyBiosource |
0.05mg |
EUR 405 |
Recombinant Mouse LIF |
MBS7609292-02mg |
MyBiosource |
0.2mg |
EUR 760 |
Recombinant Mouse LIF |
MBS7609292-1mg |
MyBiosource |
1mg |
EUR 2175 |
Recombinant Mouse LIF |
MBS7609292-5x1mg |
MyBiosource |
5x1mg |
EUR 8410 |
Recombinant Mouse LIF |
P1174-.005 |
ApexBio |
5ug |
EUR 108.8 |
Description: Peptides & Proteins|Recombinant Proteins |
Recombinant Mouse LIF |
P1174-.1 |
ApexBio |
100ug |
EUR 840.8 |
Description: Peptides & Proteins|Recombinant Proteins |
Recombinant Mouse LIF |
P1174-.5 |
ApexBio |
500ug |
EUR 1885.6 |
Description: Peptides & Proteins|Recombinant Proteins |
Recombinant Mouse LIF |
Z200195 |
ABM |
10 µg |
EUR 85 |
Description: LIF is a multifunctional secreted glycoprotein that exists in both soluble and matrix-bound forms. It displays biologic activities ranging from the differentiation of myeloid leukemic cells into macrophage lineage to effects on bone metabolism, inflammation, neural development, embryogenesis, and the maintenance of implantation. It is now clear that LIF is related in both structure and mechanism of action to the interleukin IL-6 family of cytokines, which also includes IL-11, ciliary neurotrophic factor, oncostatin M, and cardiotrophin 1. The actions of these cytokines are mediated through specific cell-surface receptors that consist of a unique chain and the shared signal transducing subunit gp130. |
Recombinant Mouse LIF |
Z200197 |
ABM |
100 µg |
EUR 385 |
Description: LIF is a multifunctional secreted glycoprotein that exists in both soluble and matrix-bound forms. It displays biologic activities ranging from the differentiation of myeloid leukemic cells into macrophage lineage to effects on bone metabolism, inflammation, neural development, embryogenesis, and the maintenance of implantation. It is now clear that LIF is related in both structure and mechanism of action to the interleukin IL-6 family of cytokines, which also includes IL-11, ciliary neurotrophic factor, oncostatin M, and cardiotrophin 1. The actions of these cytokines are mediated through specific cell-surface receptors that consist of a unique chain and the shared signal transducing subunit gp130. |
Recombinant Mouse LIF |
Z200199 |
ABM |
1.0 mg |
EUR 1200 |
Description: LIF is a multifunctional secreted glycoprotein that exists in both soluble and matrix-bound forms. It displays biologic activities ranging from the differentiation of myeloid leukemic cells into macrophage lineage to effects on bone metabolism, inflammation, neural development, embryogenesis, and the maintenance of implantation (2). It is now clear that LIF is related in both structure and mechanism of action to the interleukin IL-6 family of cytokines, which also includes IL-11, ciliary neurotrophic factor, oncostatin M, and cardiotrophin 1 (2). The actions of these cytokines are mediated through specific cell-surface receptors that consist of a unique chain and the shared signal transducing subunit gp130. |
Recombinant Mouse LIF |
Z200485 |
ABM |
100 µg |
EUR 1500 |
Description: LIF is a multifunctional secreted glycoprotein that exists in both soluble and matrix-bound forms. It displays biologic activities ranging from the differentiation of myeloid leukemic cells into macrophage lineage to effects on bone metabolism, inflammation, neural development, embryogenesis, and the maintenance of implantation (2). It is now clear that LIF is related in both structure and mechanism of action to the interleukin IL-6 family of cytokines, which also includes IL-11, ciliary neurotrophic factor, oncostatin M, and cardiotrophin 1 (2). The actions of these cytokines are mediated through specific cell-surface receptors that consist of a unique chain and the shared signal transducing subunit gp130. |
Anti-Mouse LIF Antibody |
103-M263 |
ReliaTech |
100 µg |
EUR 399 |
Description: Based on its helical structure, LIF (Leukemia Inhibitory Factor) is considered a member of the Interleukin-6 family of cytokines. Functionally, it has been implicated in a many physiological processes including development, hematopoiesis, bone metabolism, and inflammation. Some cell types known to express LIF include activated T cells, monocytes, astrocytes, osteoblasts, keratinocytes, regenerating skeletal muscle, mast cells, and fibroblasts. |
Anti-Mouse LIF Antibody |
103-PA05S |
ReliaTech |
100 µg |
EUR 126 |
Description: Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Human LIF is as active on human cells as is it is on mouse cells, though mouse LIF is about 1000 fold less active on human cells, than human LIF. |
LIF (mouse), (recombinant) |
MBS566073-1mL |
MyBiosource |
1mL |
EUR 1470 |
LIF (mouse), (recombinant) |
MBS566073-5x1mL |
MyBiosource |
5x1mL |
EUR 6555 |
Mouse LIF ELISA Kit |
E28L0650 |
EnoGene |
96T |
EUR 666.67 |
Mouse LIF ELISA Kit |
EK5250 |
SAB |
96 tests |
EUR 599 |
|
Mouse LIF ELISA Kit |
EML0014 |
Abclonal |
96Tests |
EUR 625.2 |
Mouse LIF shRNA Plasmid |
20-abx971321 |
Abbexa |
-
Ask for price
-
Ask for price
|
|
|
Lif (NM_008501) Mouse Recombinant Protein |
E45M42302E0 |
EnoGene |
20 ug |
EUR 1088.75 |
Lif (NM_008501) Mouse Recombinant Protein |
E45M43185M5 |
EnoGene |
20 ug |
EUR 1924.54 |
LIF, Human, Monoclonal Antibody, Mouse |
MBS555094-01mg |
MyBiosource |
0.1mg |
EUR 745 |
LIF, Human, Monoclonal Antibody, Mouse |
MBS555094-5x01mg |
MyBiosource |
5x0.1mg |
EUR 3210 |
Recombinant Mouse LIF protein |
CD00323-5ug |
Jiaxing Korain Biotech Ltd (BT Labs) |
5ug |
EUR 176 |
|
Description: Leukemia inhibitory factor (LIF) is a member of Interleukin 6 family. This protein is mainly expressed in the trophectoderm of the developing embryo, with its receptor LIFR expressed throughout the inner cell mass. LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. LIF is used in mouse embryonic stem cell culture, because that removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. It is also used in phase II clinical trial, which can assist embryo implantation in women who have failed to become pregnant despite assisted reproductive technologies (ART). Mature mouse LIF shares 78 % a.a. sequence identity with Human LIF. |
Mouse LIF Recombinant Protein |
M30-007 |
ReliaTech |
10 µg |
EUR 99.75 |
Description: Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Human LIF is as active on human cells as is it is on mouse cells, though mouse LIF is about 1000 fold less active on human cells, than human LIF. Recombinant mouse LIF produced in E. coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 19.86 kDa. |
Mouse LIF Recombinant Protein |
M30-008 |
ReliaTech |
50 µg |
EUR 246.75 |
Description: Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Human LIF is as active on human cells as is it is on mouse cells, though mouse LIF is about 1000 fold less active on human cells, than human LIF. Recombinant mouse LIF produced in E. coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 19.86 kDa. |
Recombinant Mouse LIF protein |
IMP-1308 |
Creative BioMart |
10µg |
EUR 360 |
|
Description: Recombinant Proteins |
Recombinant Mouse LIF Protein |
MBS1751002-01mg |
MyBiosource |
0.1mg |
EUR 315 |
Recombinant Mouse LIF Protein |
MBS1751002-5x01mg |
MyBiosource |
5x0.1mg |
EUR 1270 |
Recombinant Mouse LIF Protein |
MBS8249390-001mg |
MyBiosource |
0.01mg |
EUR 230 |
Recombinant Mouse LIF Protein |
MBS8249390-005mg |
MyBiosource |
0.05mg |
EUR 480 |
Recombinant Mouse LIF Protein |
MBS8249390-05mg |
MyBiosource |
0.5mg |
EUR 2825 |
Recombinant Mouse LIF Protein |
MBS8249390-5x05mg |
MyBiosource |
5x0.5mg |
EUR 12640 |
Recombinant Mouse LIF Protein |
PKSM041100-100ug |
Elabscience Biotech |
100ug |
EUR 739 |
|
Description: Mouse |
Recombinant Mouse LIF Protein |
PKSM041100-20ug |
Elabscience Biotech |
20ug |
EUR 249 |
|
Description: Mouse |
Recombinant Mouse LIF Protein |
R01400-1 |
BosterBio |
100ug/vial |
EUR 240 |
|
Description: SPLPI TPVNA TCAIR HPCHG NLMNQ IKNQL AQLNG SANAL FISYY TAQGE PFPNN VEKLC APNMT DFPSF HGNGT EKTKL VELYR MVAYL SASLT NITRD QKVLN PTAVS LQVKL NATID VMRGL LSNVL CRLCN KYRVG HVDVP PVPDH SDKEA FQRKK LGCQL LGTYK QVISV VVQAF |
LIF Protein, Mouse, Recombinant |
E45M52739M0-20-ug |
EnoGene |
20 ug |
EUR 291.15 |
Anti-LIF Antibody, Mouse Monoclonal |
MBS8107490-01mL |
MyBiosource |
0.1mL |
EUR 300 |
Anti-LIF Antibody, Mouse Monoclonal |
MBS8107490-5x01mL |
MyBiosource |
5x0.1mL |
EUR 1200 |
anti- LIF antibody |
FNab09804 |
FN Test |
100µg |
EUR 658.5 |
|
Description: Antibody raised against LIF |
Mouse LIF PicoKine ELISA Kit |
MBS178717-5x96StripWells |
MyBiosource |
5x96-Strip-Wells |
EUR 2755 |
Mouse LIF PicoKine ELISA Kit |
MBS178717-96StripWells |
MyBiosource |
96-Strip-Wells |
EUR 600 |
Recombinant Mouse LIF, Tag Free |
PM2019-.01 |
ApexBio |
10ug |
EUR 208 |
Description: Peptides & Proteins|Recombinant Proteins |
Recombinant Mouse LIF, Tag Free |
PM2019-.1 |
ApexBio |
10X10ug |
EUR 784 |
Description: Peptides & Proteins|Recombinant Proteins |
Lif ORF Vector (Mouse) (pORF) |
ORF049145 |
ABM |
1.0 ug DNA |
EUR 607.2 |
Lif ORF Vector (Mouse) (pORF) |
ORF049146 |
ABM |
1.0 ug DNA |
EUR 607.2 |
LIF, Mouse Recombinant , 1MIU/ml |
L4500-001 |
GenDepot |
1ml |
EUR 235 |
LIF, Mouse Recombinant , 1MIU/ml |
L4500-005 |
GenDepot |
5x1ml |
EUR 890 |
LIF ELISA Kit (Mouse) (OKBB00760) |
OKBB00760 |
Aviva Systems Biology |
96 Wells |
EUR 606 |
Description: Description of target: Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The LIF was mapped gene to 22q11-q12.2 by Southern analysis of a series of mouse/human somatic cell hybrids and by in situ hybridization to the chromosomes of 2 normal males and some individuals with chromosomal rearrangements. The gene maps between the Philadelphia translocation BCR1 and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: <10pg/ml |
LIF ELISA Kit (Mouse) (OKEH04045) |
OKEH04045 |
Aviva Systems Biology |
96 Wells |
EUR 652.8 |
Description: Description of target: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.039 ng/mL |
LIF, Mouse Recombinant , 10MIU/ml |
L4501-001 |
GenDepot |
1ml |
EUR 1100 |
LIFR, Recombinant, Mouse (Leukemia Inhibitory Factor Receptor, LIF Receptor, LIF-R, D-factor/LIF Receptor, CD118) |
MBS636029-005mg |
MyBiosource |
0.05mg |
EUR 800 |
Canine Leukemia Inhibitory Issue (LIF) ELISA Package
Manufacturing of soluble bioactive mouse leukemia inhibitory issue from Escherichia coli utilizing MBP tag.
Embryonic stem cells and induced pluripotent stem cells rely on one in every of cytokines referred to as leukemia inhibitory issue (LIF) to retain their undifferentiated state and pluripotency.
anti- Integrin alpha-5 antibody |
FNab04347 |
FN Test |
100µg |
EUR 658.5 |
|
Description: Antibody raised against Integrin alpha-5 |
Human Integrin alpha-5 ELISA Kit |
EK4913 |
SAB |
96 tests |
EUR 739 |
|
Human Integrin alpha-5 ELISA Kit |
MBS2882218-10x96StripWells |
MyBiosource |
10x96-Strip-Wells |
EUR 4630 |
Human Integrin alpha-5 ELISA Kit |
MBS2882218-48StripWells |
MyBiosource |
48-Strip-Wells |
EUR 440 |
Human Integrin alpha-5 ELISA Kit |
MBS2882218-5x96StripWells |
MyBiosource |
5x96-Strip-Wells |
EUR 2480 |
Human Integrin alpha-5 ELISA Kit |
MBS2882218-96StripWells |
MyBiosource |
96-Strip-Wells |
EUR 580 |
Human Integrin alpha-5 ELISA Kit |
MBS761812-10x96StripWells |
MyBiosource |
10x96-Strip-Wells |
EUR 3900 |
Human Integrin alpha-5 ELISA Kit |
MBS761812-48StripWells |
MyBiosource |
48-Strip-Wells |
EUR 340 |
Human Integrin alpha-5 ELISA Kit |
MBS761812-5x96StripWells |
MyBiosource |
5x96-Strip-Wells |
EUR 2045 |
Human Integrin alpha-5 ELISA Kit |
MBS761812-96StripWells |
MyBiosource |
96-Strip-Wells |
EUR 455 |
Human Integrin alpha-5 ELISA Kit |
MBS9428739-5x96Tests |
MyBiosource |
5x96Tests |
EUR 3385 |
Human Integrin alpha-5 ELISA Kit |
MBS9428739-96Tests |
MyBiosource |
96Tests |
EUR 735 |
Human Integrin Alpha 5 ELISA Kit |
E01A5456 |
BlueGene |
96T |
EUR 700 |
Description: ELISA |
Human Integrin Alpha 5 ELISA Kit |
MBS094108-10x96StripWells |
MyBiosource |
10x96-Strip-Wells |
EUR 6725 |
Human Integrin Alpha 5 ELISA Kit |
MBS094108-48StripWells |
MyBiosource |
48-Strip-Wells |
EUR 550 |
Human Integrin Alpha 5 ELISA Kit |
MBS094108-5x96StripWells |
MyBiosource |
5x96-Strip-Wells |
EUR 3420 |
Human Integrin Alpha 5 ELISA Kit |
MBS094108-96StripWells |
MyBiosource |
96-Strip-Wells |
EUR 765 |
Human Integrin Alpha 5 ELISA Kit |
IHUITGA5KT |
Innovative research |
each |
EUR 700 |
|
Description: Human Integrin Alpha 5 ELISA Kit |
Human Integrin Alpha 5 ELISA Kit |
MBS3802124-10x96StripWells |
MyBiosource |
10x96-Strip-Wells |
EUR 6725 |
Human Integrin Alpha 5 ELISA Kit |
MBS3802124-48StripWells |
MyBiosource |
48-Strip-Wells |
EUR 550 |
Human Integrin Alpha 5 ELISA Kit |
MBS3802124-5x96StripWells |
MyBiosource |
5x96-Strip-Wells |
EUR 3420 |
Human Integrin Alpha 5 ELISA Kit |
MBS3802124-96StripWells |
MyBiosource |
96-Strip-Wells |
EUR 765 |
Human Integrin Alpha 5 ELISA Kit |
MBS7273513-10x96StripWells |
MyBiosource |
10x96-Strip-Wells |
EUR 5685 |
Human Integrin Alpha 5 ELISA Kit |
MBS7273513-48StripWells |
MyBiosource |
48-Strip-Wells |
EUR 485 |
Human Integrin Alpha 5 ELISA Kit |
MBS7273513-5x96StripWells |
MyBiosource |
5x96-Strip-Wells |
EUR 3020 |
Human Integrin Alpha 5 ELISA Kit |
MBS7273513-96StripWells |
MyBiosource |
96-Strip-Wells |
EUR 690 |
Human Integrin Alpha 5 ELISA Kit |
E01I0788-48wellsplate |
BlueGene |
48 wells plate |
EUR 280 |
Human Integrin Alpha 5 ELISA Kit |
E01I0788-96wellsplate |
BlueGene |
96 wells plate |
EUR 405 |
Human Integrin Alpha 5 (ITGa5) Protein |
abx067272-100g |
Abbexa |
100 µg |
EUR 550 |
Human Integrin Alpha 5 (ITGa5) Protein |
20-abx067272 |
Abbexa |
-
Ask for price
-
Ask for price
-
Ask for price
-
Ask for price
-
Ask for price
|
- 10 ug
- 50 ug
- 100 ug
- 200 ug
- 1 mg
|
|
Human Integrin Alpha 5 (ITGa5) Protein |
abx067272-10g |
Abbexa |
10 µg |
EUR 212.5 |
Human Integrin Alpha 5 (ITGa5) Protein |
abx067272-50g |
Abbexa |
50 µg |
EUR 387.5 |
Mouse Anti Human Integrin Alpha V Beta 5 Monoclonal Antibody |
CABT-49626MH |
Creative Diagnostics |
0.1 mg |
EUR 1058.4 |
|
Description: Mouse |
ELISA Kit for Human Integrin alpha-5 |
E9148h |
EIAab |
96T |
EUR 441.6 |
Human Integrin alpha-5(ITGA5) Elisa kit |
CSB-E17350h-24T |
Cusabio |
1 plate of 24 wells |
EUR 198 |
|
Description: Quantitativesandwich ELISA kit for measuring Human Integrin alpha-5 (ITGA5) in samples from serum, plasma, cell culture supernates, urine, cerebrospinalfluid (CSF). A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Human Integrin alpha-5(ITGA5) Elisa kit |
1-CSB-E17350h |
Cusabio |
-
Ask for price
-
Ask for price
-
Ask for price
|
- 5 plates of 96 wells each
- 1 plate of 96 wells
- 10 plates of 96 wells each
|
|
Description: Quantitativesandwich ELISA kit for measuring Human Integrin alpha-5(ITGA5) in samples from serum, plasma, cell culture supernates, urine, cerebrospinalfluid(CSF). Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
Human Integrin alpha-5,ITGA5 ELISA KIT |
JOT-EK3734Hu |
Jotbody |
96 wells |
Ask for price |
Description: Human |
Human Integrin alpha-5,ITGA5 ELISA KIT |
JOT-EK5482Hu |
Jotbody |
96 wells |
Ask for price |
Description: Human |
Human Integrin alpha-5, ITGA5 ELISA Kit |
MBS1606388-10x96StripWells |
MyBiosource |
10x96-Strip-Wells |
EUR 3460 |
Human Integrin alpha-5, ITGA5 ELISA Kit |
MBS1606388-48StripWells |
MyBiosource |
48-Strip-Wells |
EUR 285 |
Human Integrin alpha-5, ITGA5 ELISA Kit |
MBS1606388-5x96StripWells |
MyBiosource |
5x96-Strip-Wells |
EUR 1750 |
Human Integrin alpha-5, ITGA5 ELISA Kit |
MBS1606388-96StripWells |
MyBiosource |
96-Strip-Wells |
EUR 425 |
Human Integrin alpha-5 (ITGA5) ELISA Kit |
RK11332 |
Abclonal |
96T |
EUR 322.37 |
Mouse anti Integrin alpha 5 (CD49e) |
MBS570237-01mg |
MyBiosource |
0.1mg |
EUR 515 |
Mouse anti Integrin alpha 5 (CD49e) |
MBS570237-5x01mg |
MyBiosource |
5x0.1mg |
EUR 2165 |
Human Integrin Alpha 5 (ITGa5) CLIA Kit |
20-abx492595 |
Abbexa |
-
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|
- 96 tests
- 5 × 96 tests
- 10 × 96 tests
|
|
Integrin alpha-5 |
AP84250 |
SAB |
1mg |
EUR 2640 |
|
Integrin alpha-5 |
AP84313 |
SAB |
1mg |
EUR 2640 |
|
Integrin alpha-5 |
AP84459 |
SAB |
1mg |
EUR 2640 |
|
Human Integrin Alpha 5 (ITGA5) ELISA Kit |
abx571172-96tests |
Abbexa |
96 tests |
EUR 886.8 |
|
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
20-abx151987 |
Abbexa |
-
Ask for price
-
Ask for price
-
Ask for price
|
- 96 tests
- 5 × 96 tests
- 10 × 96 tests
|
|
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
DL-ITGa5-Hu |
DL Develop |
96T |
EUR 421 |
|
Description: tissue homogenates, cell lysates or other biological fluids. |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
EK15036 |
SAB |
96Т |
EUR 768 |
|
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
DLR-ITGa5-Hu |
DL Develop |
96T |
EUR 443 |
|
Description: tissue homogenates, cell lysates or other biological fluids. |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
DLR-ITGa5-Hu-48T |
DL Develop |
48T |
EUR 597.6 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Integrin Alpha 5 (ITGa5) in samples from tissue homogenates, cell lysates or other biological fluids. |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
DLR-ITGa5-Hu-96T |
DL Develop |
96T |
EUR 776.4 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Integrin Alpha 5 (ITGa5) in samples from tissue homogenates, cell lysates or other biological fluids. |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
MBS2023182-10x96StripWells |
MyBiosource |
10x96-Strip-Wells |
EUR 5100 |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
MBS2023182-24StripWells |
MyBiosource |
24-Strip-Wells |
EUR 350 |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
MBS2023182-48StripWells |
MyBiosource |
48-Strip-Wells |
EUR 520 |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
MBS2023182-5x96StripWells |
MyBiosource |
5x96-Strip-Wells |
EUR 2795 |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
MBS2023182-96StripWells |
MyBiosource |
96-Strip-Wells |
EUR 695 |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
MBS2703128-10x96StripWells |
MyBiosource |
10x96-Strip-Wells |
EUR 2980 |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
MBS2703128-24StripWells |
MyBiosource |
24-Strip-Wells |
EUR 245 |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
MBS2703128-48StripWells |
MyBiosource |
48-Strip-Wells |
EUR 325 |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
MBS2703128-5x96StripWells |
MyBiosource |
5x96-Strip-Wells |
EUR 1555 |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
MBS2703128-96StripWells |
MyBiosource |
96-Strip-Wells |
EUR 395 |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
MBS455685-10x96StripWells |
MyBiosource |
10x96-Strip-Wells |
EUR 4890 |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
MBS455685-48StripWells |
MyBiosource |
48-Strip-Wells |
EUR 425 |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
MBS455685-5x96StripWells |
MyBiosource |
5x96-Strip-Wells |
EUR 2495 |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
MBS455685-96StripWells |
MyBiosource |
96-Strip-Wells |
EUR 560 |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
MBS4500517-10x96Tests |
MyBiosource |
10x96Tests |
EUR 5060 |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
MBS4500517-48Tests |
MyBiosource |
48Tests |
EUR 445 |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
MBS4500517-5x96Tests |
MyBiosource |
5x96Tests |
EUR 2600 |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
MBS4500517-96Test |
MyBiosource |
96Test |
EUR 585 |
Human Integrin Alpha 5 (ITGa5) ELISA Kit |
RDR-ITGa5-Hu-48T |
Reddot Biotech |
48T |
EUR 476 |
|
Description: sandwich |
However, additional progresses of stem cell scientific investigation and its attainable software are restricted owing to the expense of economic LIF. Right here we launched a easy, sensible and excessive stage expression of MBP-mouse LIF by Escherichia coli system which was bioactive.
Rat Leukemia Inhibitory Issue (LIF) ELISA Package
The mLIF cDNA was inserted into vector of pMAL-C2X with a purpose to generate N-terminal MBP-mLIF recombinant proteins within the cytoplasm of Escherichia coli. MBP-mLIF as a soluble kind was expressed. One-step purification by gravitational affinity chromatography was achieved to amass excessive purity (>92%) MBP-mLIF. The MBP-mLIF merchandise particularly suppressed the expansion of M1cells in a dose-dependent sample. MBP-mLIF additionally was proved the power to keep up the pluripotency of iPSCs.
These outcomes revealed that the N-end MBP tags of the MBP-mLIF didn’t hinder mLIF bioactivity. This methodology to generate recombinant MBP-mLIF is an easy, sensible, economical and user-friendly protocol.