Effect on Mouse Leukemia Inhibitory Factor

Immunization in opposition to leukemia inhibitory issue and its receptor suppresses tumor formation of breast most cancers initiating cells in BALB/c mouse

• Immunotherapy is a promising method for particular focusing on of most cancers cells. Leukemia inhibitory issue (LIF) regulates a number of options of cancers and most cancers stem cells (CSCs) by binding to LIF receptor (LIFR). On this research, we investigated the consensus of LIF and LIFR immunization on the expansion of mouse mammary tumors. For this objective, mouse LIF and LIFR had been designed as truncated proteins, expressed in E. coli after which injected to mice as particular person and blended antigens.

 

• The outcomes confirmed the manufacturing of neutralizing antibodies and secretion of interferon-γ and interleukin-2 in response to immunization. In proceed, the immunized mice had been subjected for tumor formation problem by inoculation of the breast CSCs derived from MC4-L2 cells.

 

• Improvement of the breast tumors was noticed in all of the management mice, whereas the tumors appeared in 75% of animals within the LIF group. LIFR injection, individually or together with LIF, strongly inhibited the tumor development to solely 25% of the mice. Furthermore, a delay in tumor look was noticed within the immunized mice in comparison with the controls.

 

• Immunostaining of the tumor sections confirmed the expression of LIF and LIFR. In conclusion, LIF and LIFR is likely to be efficient targets for immunotherapy of the tumors that specific these proteins.

 

• Earlier research have proven that some inflammatory cytokines promote the expression of corticotropin-releasing hormone (CRH) in trophoblasts throughout being pregnant and that placental CRH might induce the manufacturing of adrenocorticotropic hormone (ACTH) in people.

 

• Nevertheless, whether or not the identical is true in rodent placenta stays unclear. On this research, we examined the impact of pro-inflammatory cytokine LIF on the induction of CRH in mouse trophoblast stem cells (mTSCs). Throughout differentiation, the CRH ranges in mTSCs progressively elevated.

Mouse Leukemia Inhibitory Issue (LIF) ELISA Package

• On days three and 5 after LIF supplementation, Crh expression within the differentiated mTSCs was considerably elevated with LIF remedy than these with out LIF remedy. Furthermore, the CRH focus within the tradition media elevated. Thereafter, we examined the contribution of the downstream pathways of LIF to CRH induction in differentiated mTSCs.

 

• The LIF-induced upregulation of CRH was attenuated by inhibition of PI3K/AKT and MAPK phosphorylation however not by inhibition of JAK/STAT3. Due to this fact, in mTSCs, LIF elevated Crh expression by activation of the PI3K/AKT and MAPK pathways however not by the JAK/STAT3 pathway. The current research means that mTSC is a perfect in vitro mannequin for finding out regulation and performance of placental CRH.

Bovine Leukemia Inhibitory Issue (LIF) ELISA Package

Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Mu-48Tests	Reddot Biotech	48 Tests	EUR 511

Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Mu-96Tests	Reddot Biotech	96 Tests	EUR 709

Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Mu-48Tests	Reddot Biotech	48 Tests	EUR 489

Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Mu-96Tests	Reddot Biotech	96 Tests	EUR 677

anti-LIF
YF-PA12952	Abfrontier	50 ug	EUR 363
Description: Mouse polyclonal to LIF

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-b-48T	DL Develop	48T	EUR 547
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-b-96T	DL Develop	96T	EUR 715
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-c-48T	DL Develop	48T	EUR 527
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-c-96T	DL Develop	96T	EUR 688
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-Hu-48T	DL Develop	48T	EUR 380
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-Hu-96T	DL Develop	96T	EUR 485
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-p-48T	DL Develop	48T	EUR 547
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-p-96T	DL Develop	96T	EUR 715
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-Ra-48T	DL Develop	48T	EUR 508
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-Ra-96T	DL Develop	96T	EUR 661
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-b-48Tests	Reddot Biotech	48 Tests	EUR 580

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-b-96Tests	Reddot Biotech	96 Tests	EUR 807

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-c-48Tests	Reddot Biotech	48 Tests	EUR 557

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-c-96Tests	Reddot Biotech	96 Tests	EUR 774

Human Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Hu-48Tests	Reddot Biotech	48 Tests	EUR 383

Human Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Hu-96Tests	Reddot Biotech	96 Tests	EUR 525

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-p-48Tests	Reddot Biotech	48 Tests	EUR 580

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-p-96Tests	Reddot Biotech	96 Tests	EUR 807

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Ra-48Tests	Reddot Biotech	48 Tests	EUR 534

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Ra-96Tests	Reddot Biotech	96 Tests	EUR 742

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-b-48Tests	Reddot Biotech	48 Tests	EUR 555

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-b-96Tests	Reddot Biotech	96 Tests	EUR 771

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-c-48Tests	Reddot Biotech	48 Tests	EUR 533

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-c-96Tests	Reddot Biotech	96 Tests	EUR 740

Human Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Hu-48Tests	Reddot Biotech	48 Tests	EUR 367

Human Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Hu-96Tests	Reddot Biotech	96 Tests	EUR 502

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-p-48Tests	Reddot Biotech	48 Tests	EUR 555

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-p-96Tests	Reddot Biotech	96 Tests	EUR 771

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Ra-48Tests	Reddot Biotech	48 Tests	EUR 511

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Ra-96Tests	Reddot Biotech	96 Tests	EUR 709

Anti-LIF Antibody
PA1562	BosterBio	100ug/vial	EUR 294

Anti-LIF Antibody
PB9036	BosterBio	100ug/vial	EUR 334

Anti-LIF Antibody
A01400	BosterBio	100ug/vial	EUR 294

Anti-LIF antibody
STJ96472	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to LIF.

Anti-LIF antibody
STJ73631	St John's Laboratory	100 µg	EUR 359

Anti-LIF antibody
STJ29846	St John's Laboratory	100 µl	EUR 277
Description: The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

anti- LIF antibody
FNab09804	FN Test	100µg	EUR 548.75
Description: Antibody raised against LIF

anti- LIF antibody
LSMab09804	Lifescience Market	100 ug	EUR 386

Rabbit Polyclonal antibody Anti-CRBN
Anti-CRBN	ImmunoStep	50 µg	EUR 349

LIF, Mouse
HY-P7084	MedChemExpress	50ug	EUR 533

LIF, mouse
MO16102	Neuromics	100 ug	EUR 675

Anti-LIF (aa28-39) antibody
STJ72919	St John's Laboratory	100 µg	EUR 359

Polyclonal Goat anti-GST α-form
GST-ANTI-1	Detroit R&D	50 uL	EUR 280

Polyclonal Goat anti-GST μ-form
GST-ANTI-2	Detroit R&D	50 uL	EUR 280

Polyclonal Goat anti-GST p-form
GST-ANTI-3	Detroit R&D	50 uL	EUR 280

Mouse LIF ELISA Kit
EML0014	Abclonal	96Tests	EUR 521

Mouse LIF shRNA Plasmid
20-abx971321	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg

Recombinant Mouse LIF Protein
R01400-1	BosterBio	100ug/vial	EUR 294

LIF, Human
HY-P7049	MedChemExpress	10ug	EUR 1600

LIF siRNA
20-abx922546	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

LIF siRNA
20-abx922547	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

LIF Antibody
25283-100ul	SAB	100ul	EUR 390

LIF Antibody
36950-100ul	SAB	100ul	EUR 252

LIF, human
MO16101	Neuromics	100 ug	EUR 688

LIF Antibody
1-CSB-PA198481	Cusabio	EUR 317.00 EUR 244.00	100ul 50ul
Description: A polyclonal antibody against LIF. Recognizes LIF from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:10000, IHC:1:25-1:100

LIF Antibody
1-CSB-PA012928ESR2HU	Cusabio	EUR 222.00 EUR 335.00	100ul 50ul
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: ELISA

LIF Antibody
1-CSB-PA004761	Cusabio	EUR 222.00 EUR 195.00	100ug 50ug
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000

rHu LIF
AK9977-0005	Akron Biotech	5µg	Ask for price

rHu LIF
AK9977-0025	Akron Biotech	25µg	Ask for price

rHu LIF
AK9977-0100	Akron Biotech	100µg	Ask for price

rHu LIF
AK9977-1000	Akron Biotech	1mg	Ask for price

LIF Antibody
5216-100	Biovision		EUR 316

LIF Antibody
5216-30T	Biovision		EUR 146

LIF antibody
PAab09804	Lifescience Market	100 ug	EUR 386

LIF antibody
70R-6230	Fitzgerald	50 ug	EUR 467
Description: Rabbit polyclonal LIF antibody

LIF protein
30R-2771	Fitzgerald	10 ug	EUR 369
Description: Purified recombinant Human LIF protein

LIF protein
30R-2772	Fitzgerald	10 ug	EUR 489
Description: Purified recombinant Mouse LIF protein

LIF antibody
70R-12299	Fitzgerald	100 ug	EUR 403
Description: Rabbit polyclonal LIF antibody

LIF antibody
70R-14100	Fitzgerald	100 ug	EUR 322
Description: Affinity purified Rabbit polyclonal LIF antibody

LIF antibody
70R-51295	Fitzgerald	100 ul	EUR 244
Description: Purified Polyclonal LIF antibody

LIF Peptide
6245P	ProSci	0.05 mg	EUR 164.75
Description: (IN) LIF peptide

LIF Peptide
43-200P	ProSci	0.1 mg	EUR 338

LIF Antibody
6245-002mg	ProSci	0.02 mg	EUR 171.82
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.

LIF Antibody
6245-01mg	ProSci	0.1 mg	EUR 436.42
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.

Mouse LIF PicoKine ELISA Kit
EK0580	BosterBio	96 wells	EUR 425
Description: For quantitative detection of mouse LIF in cell culture supernates, serum and plasma (heparin, EDTA).

LIF ELISA Kit (Mouse) (OKBB00760)
OKBB00760	Aviva Systems Biology	96 Wells	EUR 505
Description: Description of target: Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The LIF was mapped gene to 22q11-q12.2 by Southern analysis of a series of mouse/human somatic cell hybrids and by in situ hybridization to the chromosomes of 2 normal males and some individuals with chromosomal rearrangements. The gene maps between the Philadelphia translocation BCR1 and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: <10pg/ml

LIF ELISA Kit (Mouse) (OKEH04045)
OKEH04045	Aviva Systems Biology	96 Wells	EUR 544
Description: Description of target: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.039 ng/mL

ELISA kit for Mouse LIF
EK5250	SAB	96 tests	EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse LIF in samples from serum, plasma, tissue homogenates and other biological fluids.

LIF, Mouse Recombinant , 1MIU/ml
L4500-001	GenDepot	1ml	EUR 339

LIF, Mouse Recombinant , 1MIU/ml
L4500-005	GenDepot	5x1ml	EUR 1193

LIF, Mouse Recombinant , 10MIU/ml
L4501-001	GenDepot	1ml	EUR 1454

Leukemia Inhibitory Factor (LIF), mouse
PR16102	Neuromics	5 ug	EUR 435

Human CellExp? LIF, mouse recombinant
7268-10	Biovision		EUR 370

Lif ORF Vector (Mouse) (pORF)
ORF049145	ABM	1.0 ug DNA	EUR 506

Lif ORF Vector (Mouse) (pORF)
ORF049146	ABM	1.0 ug DNA	EUR 506

 

Canine Leukemia Inhibitory Issue (LIF) ELISA Package

 

Manufacturing of soluble bioactive mouse leukemia inhibitory issue from Escherichia coli utilizing MBP tag.

Embryonic stem cells and induced pluripotent stem cells rely on one in every of cytokines referred to as leukemia inhibitory issue (LIF) to retain their undifferentiated state and pluripotency.

Integrin alpha 5 Antibody
49269-50ul	SAB	50ul	EUR 239

Alpha V Beta 5 Integrin, Peptide Aptamer, Biotinylated
AP-301-B	Alpha Diagnostics	1 mg	Ask for price

Alpha V Beta 5 Integrin, Peptide Aptamer, unlabeled
AP-301-U	Alpha Diagnostics	5 mg	Ask for price

Anti-Integrin alpha 5 Rabbit Monoclonal Antibody
M01911	BosterBio	100ug/vial	EUR 397
Description: Rabbit Monoclonal Integrin alpha 5 Antibody. Validated in IP, IF, WB and tested in Human, Mouse, Rat.

Alpha V Beta 5 Integrin, Peptide Aptamer, FITC labelled
AP-301-F	Alpha Diagnostics	1 mg	Ask for price

Human Integrin Alpha 5 (ITGa5) Protein
20-abx067272	Abbexa	EUR 662.00 EUR 272.00 EUR 2040.00 EUR 787.00 EUR 481.00	100 ug 10 ug 1 mg 200 ug 50 ug

anti-Integrin beta 5
YF-PA24023	Abfrontier	50 ul	EUR 334
Description: Mouse polyclonal to Integrin beta 5

anti-Integrin beta 5
YF-PA12792	Abfrontier	100 ug	EUR 403
Description: Rabbit polyclonal to Integrin beta 5

Integrin Alpha 5 (ITGa5) Antibody
20-abx172956	Abbexa	EUR 815.00 EUR 425.00	1 mg 200 ug

Integrin Alpha 5 (ITGa5) Antibody
20-abx129339	Abbexa	EUR 425.00 EUR 133.00 EUR 1177.00 EUR 578.00 EUR 328.00	100 ug 10 ug 1 mg 200 ug 50 ug

Integrin Alpha 5 (ITGA5) Antibody
20-abx113185	Abbexa	EUR 732.00 EUR 398.00	150 ul 50 ul

Integrin Alpha 5 (ITGa5) Antibody
20-abx101634	Abbexa	EUR 411.00 EUR 133.00 EUR 1149.00 EUR 565.00 EUR 314.00	100 ug 10 ug 1 mg 200 ug 50 ug

Integrin Alpha 5 (ITGa5) Antibody
20-abx101635	Abbexa	EUR 467.00 EUR 133.00 EUR 1344.00 EUR 634.00 EUR 356.00	100 ug 10 ug 1 mg 200 ug 50 ug

Integrin Alpha 5 (ITGA5) Antibody
abx011678-100ul	Abbexa	100 ul	EUR 411

Integrin Alpha 5 (ITGA5) Antibody
20-abx006811	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

Integrin Alpha 5 (ITGA5) Antibody
20-abx000873	Abbexa	EUR 411.00 EUR 592.00	100 ul 200 ul

Integrin Alpha 5 (ITGA5) Antibody
abx026655-400ul	Abbexa	400 ul	EUR 523

Integrin Alpha 5 (ITGA5) Antibody
abx026655-80l	Abbexa	80 µl	EUR 286

Integrin alpha 5 Rabbit mAb
A19069-100ul	Abclonal	100 ul	EUR 410

Integrin alpha 5 Rabbit mAb
A19069-200ul	Abclonal	200 ul	EUR 571

Integrin alpha 5 Rabbit mAb
A19069-20ul	Abclonal	20 ul	EUR 221

Integrin alpha 5 Rabbit mAb
A19069-50ul	Abclonal	50 ul	EUR 287

Integrin Alpha 5 (ITGa5) Antibody
20-abx179024	Abbexa	EUR 1678.00 EUR 773.00	1 mg 200 ug

Integrin Alpha 5 (ITGA5) Antibody
20-abx323564	Abbexa	EUR 314.00 EUR 244.00	100 ug 50 ug

Integrin alpha 5 (CD49e) Antibody
abx216264-100ug	Abbexa	100 ug	EUR 439

Integrin Alpha 5 (ITGA5) Antibody
20-abx338742	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

Integrin Alpha 5 (ITGA5) Antibody
20-abx327245	Abbexa	EUR 314.00 EUR 244.00	100 ug 50 ug

Integrin Alpha 5 (ITGA5) Antibody
20-abx325454	Abbexa	EUR 314.00 EUR 244.00	100 ug 50 ug

Integrin alpha 5 Conjugated Antibody
C49269	SAB	100ul	EUR 397

Recombinant Integrin Alpha 5 (ITGa5)
4-RPB287Bo01	Cloud-Clone	EUR 556.96 EUR 252.00 EUR 1813.60 EUR 671.20 EUR 1242.40 EUR 436.00 EUR 4384.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Bovine Integrin Alpha 5 expressed in: E.coli

Recombinant Integrin Alpha 5 (ITGa5)
4-RPB287Hu01	Cloud-Clone	EUR 476.32 EUR 230.00 EUR 1511.20 EUR 570.40 EUR 1040.80 EUR 382.00 EUR 3628.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Integrin Alpha 5 expressed in: E.coli

Recombinant Integrin Alpha 5 (ITGa5)
4-RPB287Mu01	Cloud-Clone	EUR 476.32 EUR 230.00 EUR 1511.20 EUR 570.40 EUR 1040.80 EUR 382.00 EUR 3628.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Mouse Integrin Alpha 5 expressed in: E.coli

Integrin alpha 5 (CD49e) Antibody
AF5200	Affbiotech	200ul	EUR 304
Description: Integrin α5 (CD49e) Antibody detects endogenous levels of total Integrin α5 (CD49e).

Integrin alpha-5 Recombinant Protein
91-433	ProSci	0.05 mg	EUR 542.75
Description: Integrin alpha-5 belongs to the Integrin alpha chain family and contains 7 FG-GAP repeats. Integrin alpha-5 joins with Integrin- beta 1 to form a fibronectin and laminin receptor which recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. It is expressed on fibroblasts, endothelial cells, peripheral T cells and platelets. Integrin alpha-5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains. In addition to adhesion, ITGA5 participates in cell-surface mediated signalling.

Rabbit Anti-Human Alpha Defensin 5 (NP-5),antiserum #1
HDEFA51-S	Alpha Diagnostics	100 ul	EUR 457

Anti-Integrin alpha antibody
STJ93727	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to Integrin alpha3.

Anti-Integrin alpha antibody
STJ93728	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to Integrin alpha4.

Anti-Integrin alpha antibody
STJ93729	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to Integrin alphaE.

Anti-Integrin alpha antibody
STJ93730	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to Integrin alphaV.

Anti-Integrin alpha antibody
STJ98181	St John's Laboratory	100 µl	EUR 234
Description: Mouse monoclonal to Integrin alpha5.

Anti-Integrin alpha antibody
STJ98510	St John's Laboratory	100 µl	EUR 234
Description: Mouse monoclonal to Integrin alpha5.

Anti-Integrin alpha antibody
STJ98511	St John's Laboratory	100 µl	EUR 234
Description: Mouse monoclonal to Integrin alpha6.

Anti-Integrin alpha antibody
STJ98709	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to Integrin alpha1.

Anti-Integrin alpha antibody
STJ98737	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to Integrin alphaX.

Anti-Integrin alpha antibody
STJ98984	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to Integrin alphaE.

Anti-Integrin alpha antibody
STJ96613	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to Integrin alpha4.

Anti-Integrin alpha antibody
STJ96825	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to Integrin alphaD.

Anti-Integrin alpha antibody
STJ97273	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to Integrin alphaL.

Anti-Integrin alpha antibody
STJ97283	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to Integrin alpha2.

Anti-Integrin alpha antibody
STJ97284	St John's Laboratory	200 µl	EUR 197
Description: Integrin alpha5 is a protein encoded by the ITGA5 gene which is approximately 114,5 kDa. Integrin alpha5 is localised to the cell membrane and is involved in the MAPK-Erk pathway, apoptotic pathways, integrin pathway, focal adhesion and blood-brain barrier and immune cell transmigration. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signalling. Integrin alpha5 is expressed in the liver, lung, nervous system, bone marrow and blood. Mutations in the ITGA5 gene may result in colon carcinoma and congenital epulis STJ97284 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Integrin alpha5 protein.

Anti-Integrin alpha antibody
STJ97285	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to Integrin alpha6.

Anti-Integrin alpha antibody
STJ97657	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to Integrin alpha3.

anti-Integrin alpha 6
YF-PA24004	Abfrontier	50 ul	EUR 334
Description: Mouse polyclonal to Integrin alpha 6

anti-Integrin alpha 1
YF-PA24011	Abfrontier	50 ul	EUR 334
Description: Mouse polyclonal to Integrin alpha 1

anti-Integrin alpha 4
YF-PA24013	Abfrontier	50 ul	EUR 334
Description: Mouse polyclonal to Integrin alpha 4

anti-Integrin alpha 9
YF-PA24015	Abfrontier	50 ul	EUR 334
Description: Mouse polyclonal to Integrin alpha 9

anti-Integrin alpha E
YF-PA24017	Abfrontier	50 ul	EUR 334
Description: Mouse polyclonal to Integrin alpha E

anti-Integrin alpha 6
YF-PA12761	Abfrontier	50 ul	EUR 363
Description: Mouse polyclonal to Integrin alpha 6

anti-Integrin alpha 6
YF-PA12762	Abfrontier	100 ug	EUR 403
Description: Rabbit polyclonal to Integrin alpha 6

anti-Integrin alpha 1
YF-PA12784	Abfrontier	50 ug	EUR 363
Description: Mouse polyclonal to Integrin alpha 1

anti-Integrin alpha 4
YF-PA12785	Abfrontier	100 ug	EUR 403
Description: Rabbit polyclonal to Integrin alpha 4

anti-Integrin alpha V
YF-PA12786	Abfrontier	50 ul	EUR 363
Description: Mouse polyclonal to Integrin alpha V

anti-Integrin alpha V
YF-PA12787	Abfrontier	50 ug	EUR 363
Description: Mouse polyclonal to Integrin alpha V

anti-Integrin alpha V
YF-PA12788	Abfrontier	100 ug	EUR 403
Description: Rabbit polyclonal to Integrin alpha V

Mouse Anti Human Integrin Alpha V Beta 5 Monoclonal Antibody
CABT-49626MH	Creative Diagnostics	0.1 mg	EUR 1375

Anti-alpha 5 beta 1 Integrin (Volociximab), Human IgG4 Antibody
A1092-200	Biovision		EUR 729

Human Integrin Alpha 5 (ITGA5) ELISA Kit
abx571172-96tests	Abbexa	96 tests	EUR 739

Human ITGA5/ Integrin alpha-5 ELISA Kit
E1342Hu	Sunlong	1 Kit	EUR 605

Human Integrin Alpha 5 (ITGa5) CLIA Kit
20-abx492595	Abbexa	EUR 7973.00 EUR 4246.00 EUR 981.00	10 × 96 tests 5 × 96 tests 96 tests

Human Integrin Alpha 5 (ITGa5) ELISA Kit
20-abx151987	Abbexa	EUR 7112.00 EUR 3792.00 EUR 879.00	10 × 96 tests 5 × 96 tests 96 tests

Human Integrin Alpha 5 (ITGa5) ELISA Kit
DLR-ITGa5-Hu-48T	DL Develop	48T	EUR 498
Description: A sandwich quantitative ELISA assay kit for detection of Human Integrin Alpha 5 (ITGa5) in samples from tissue homogenates, cell lysates or other biological fluids.

Human Integrin Alpha 5 (ITGa5) ELISA Kit
DLR-ITGa5-Hu-96T	DL Develop	96T	EUR 647
Description: A sandwich quantitative ELISA assay kit for detection of Human Integrin Alpha 5 (ITGa5) in samples from tissue homogenates, cell lysates or other biological fluids.

Human Integrin Alpha 5 (ITGa5) ELISA Kit
RDR-ITGa5-Hu-48Tests	Reddot Biotech	48 Tests	EUR 522

Human Integrin Alpha 5 (ITGa5) ELISA Kit
RDR-ITGa5-Hu-96Tests	Reddot Biotech	96 Tests	EUR 724

Human Integrin Alpha 5 (ITGa5) ELISA Kit
RD-ITGa5-Hu-48Tests	Reddot Biotech	48 Tests	EUR 500

Human Integrin Alpha 5 (ITGa5) ELISA Kit
RD-ITGa5-Hu-96Tests	Reddot Biotech	96 Tests	EUR 692

ELISA kit for Human Integrin alpha-5
EK4913	SAB	96 tests	EUR 670
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Integrin alpha-5 in samples from serum, plasma, tissue homogenates and other biological fluids.

Human Integrin alpha-5(ITGA5) Elisa kit
CSB-E17350h-24T	Cusabio	1 plate of 24 wells	EUR 165
Description: Quantitativesandwich ELISA kit for measuring Human Integrin alpha-5 (ITGA5) in samples from serum, plasma, cell culture supernates, urine, cerebrospinalfluid (CSF). A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Integrin alpha-5(ITGA5) Elisa kit
1-CSB-E17350h	Cusabio	EUR 900.00 EUR 5476.00 EUR 2900.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Integrin alpha-5(ITGA5) in samples from serum, plasma, cell culture supernates, urine, cerebrospinalfluid(CSF). Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Integrin Alpha 5 (ITGa5) ELISA Kit
SEB287Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 4502.43
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.

Human Integrin Alpha 5 (ITGa5) ELISA Kit
SEB287Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 458.44
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.

Human Integrin Alpha 5 (ITGa5) ELISA Kit
SEB287Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 612.05
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.

Human Integrin Alpha 5 (ITGa5) ELISA Kit
SEB287Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2454.23
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.

Human Integrin Alpha 5 (ITGa5) ELISA Kit
4-SEB287Hu	Cloud-Clone	EUR 4553.00 EUR 2405.00 EUR 613.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Integrin Alpha 5 (ITGa5) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Integrin Alpha 5 (ITGa5) Polyclonal Antibody (Human)
4-PAB287Hu01	Cloud-Clone	EUR 239.00 EUR 2391.00 EUR 598.00 EUR 299.00 EUR 211.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Integrin Alpha 5 (ITGa5)

Human Integrin Alpha 5(ITGa5) ELISA Kit
QY-E05294	Qayee Biotechnology	96T	EUR 394

Human ITGA5(Integrin alpha-5) ELISA Kit
EH2445	FN Test	96T	EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Human Integrin alpha- 5, ITGA5 ELISA KIT
ELI-07691h	Lifescience Market	96 Tests	EUR 824

Cow Integrin Alpha 5 (ITGa5) Protein
20-abx067274	Abbexa	EUR 773.00 EUR 300.00 EUR 2444.00 EUR 926.00 EUR 551.00	100 ug 10 ug 1 mg 200 ug 50 ug

Integrin Alpha 5 (ITGA5) Antibody (FITC)
abx413989-01mg	Abbexa	0.1 mg	EUR 509

Mouse Integrin Alpha 5 (ITGa5) Protein
20-abx168338	Abbexa	EUR 662.00 EUR 272.00 EUR 2040.00 EUR 787.00 EUR 481.00	100 ug 10 ug 1 mg 200 ug 50 ug

Integrin Alpha 5 (ITGA5) Antibody (HRP)
20-abx336126	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

Integrin Alpha 5 (ITGA5) Antibody (FITC)
20-abx336127	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

However, additional progresses of stem cell scientific investigation and its attainable software are restricted owing to the expense of economic LIF. Right here we launched a easy, sensible and excessive stage expression of MBP-mouse LIF by Escherichia coli system which was bioactive.

Rat Leukemia Inhibitory Issue (LIF) ELISA Package

The mLIF cDNA was inserted into vector of pMAL-C2X with a purpose to generate N-terminal MBP-mLIF recombinant proteins within the cytoplasm of Escherichia coli. MBP-mLIF as a soluble kind was expressed. One-step purification by gravitational affinity chromatography was achieved to amass excessive purity (>92%) MBP-mLIF. The MBP-mLIF merchandise particularly suppressed the expansion of M1cells in a dose-dependent sample. MBP-mLIF additionally was proved the power to keep up the pluripotency of iPSCs.

 

These outcomes revealed that the N-end MBP tags of the MBP-mLIF didn’t hinder mLIF bioactivity. This methodology to generate recombinant MBP-mLIF is an easy, sensible, economical and user-friendly protocol.